Expression of human granulocyte colony stimulating factor (hG-CSF) in colon adenocarcinoma cell line (Caco-2)
Growth and progression of many cancer cells are mediated by alterations in the microenvironment often caused by an aberrant expression of growth factors and receptors. There is no report on expression of growth factor granulocyte colony-stimulating factor (G-CSF) in the experimental model, colon ade...
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| Veröffentlicht in: | Biotechnology letters 2012-10, Vol.34 (10), p.1791-1796 |
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| creator | Jana, Snehasis Patel, Hitesh |
| description | Growth and progression of many cancer cells are mediated by alterations in the microenvironment often caused by an aberrant expression of growth factors and receptors. There is no report on expression of growth factor granulocyte colony-stimulating factor (G-CSF) in the experimental model, colon adenocarcinoma cell line (Caco2), that is commonly used in drug permeability assays. We hypothesize that in vitro, the Caco2 model is associated with a constitutive neo-expression of the hematopoietic G-CSF thereby causing an autocrine stimulation of Caco2 growth and proliferation in vitro. To test our hypothesis, we analyzed mRNA and protein expression of G-CSF in Caco2 cells using reverse transcriptase-PCR and SDS-PAGE. G-CSF mRNA and protein were detected in Caco2 cells. Expression of G-CSF protein was similar at different passages of this cell line. The expression of G-CSF has a significant role in the autocrine regulation of Caco2 cell growth and proliferation. |
| doi_str_mv | 10.1007/s10529-012-0977-5 |
| format | Article |
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There is no report on expression of growth factor granulocyte colony-stimulating factor (G-CSF) in the experimental model, colon adenocarcinoma cell line (Caco2), that is commonly used in drug permeability assays. We hypothesize that in vitro, the Caco2 model is associated with a constitutive neo-expression of the hematopoietic G-CSF thereby causing an autocrine stimulation of Caco2 growth and proliferation in vitro. To test our hypothesis, we analyzed mRNA and protein expression of G-CSF in Caco2 cells using reverse transcriptase-PCR and SDS-PAGE. G-CSF mRNA and protein were detected in Caco2 cells. Expression of G-CSF protein was similar at different passages of this cell line. The expression of G-CSF has a significant role in the autocrine regulation of Caco2 cell growth and proliferation.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-012-0977-5</identifier><identifier>PMID: 22714276</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>adenocarcinoma ; Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Applied Microbiology ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Caco-2 Cells ; Cell growth ; Cell Growth Processes - physiology ; colon ; Colonic Neoplasms - genetics ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - pathology ; Colorectal cancer ; drugs ; Fundamental and applied biological sciences. Psychology ; granulocyte colony-stimulating factor ; Granulocyte Colony-Stimulating Factor - biosynthesis ; Granulocyte Colony-Stimulating Factor - genetics ; Growth factors ; Humans ; Life Sciences ; messenger RNA ; Microbiology ; Original Research Paper ; permeability ; polyacrylamide gel electrophoresis ; Polymerase chain reaction ; protein synthesis ; receptors ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Sensitivity and Specificity</subject><ispartof>Biotechnology letters, 2012-10, Vol.34 (10), p.1791-1796</ispartof><rights>The Author(s) 2012</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c614t-31d3b1fa4aca304c04c366a290a6e28cd0baedd76a442fb7583e8251de8e78143</citedby><cites>FETCH-LOGICAL-c614t-31d3b1fa4aca304c04c366a290a6e28cd0baedd76a442fb7583e8251de8e78143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-012-0977-5$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-012-0977-5$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26362916$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22714276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jana, Snehasis</creatorcontrib><creatorcontrib>Patel, Hitesh</creatorcontrib><title>Expression of human granulocyte colony stimulating factor (hG-CSF) in colon adenocarcinoma cell line (Caco-2)</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Growth and progression of many cancer cells are mediated by alterations in the microenvironment often caused by an aberrant expression of growth factors and receptors. There is no report on expression of growth factor granulocyte colony-stimulating factor (G-CSF) in the experimental model, colon adenocarcinoma cell line (Caco2), that is commonly used in drug permeability assays. We hypothesize that in vitro, the Caco2 model is associated with a constitutive neo-expression of the hematopoietic G-CSF thereby causing an autocrine stimulation of Caco2 growth and proliferation in vitro. To test our hypothesis, we analyzed mRNA and protein expression of G-CSF in Caco2 cells using reverse transcriptase-PCR and SDS-PAGE. G-CSF mRNA and protein were detected in Caco2 cells. Expression of G-CSF protein was similar at different passages of this cell line. The expression of G-CSF has a significant role in the autocrine regulation of Caco2 cell growth and proliferation.</description><subject>adenocarcinoma</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Caco-2 Cells</subject><subject>Cell growth</subject><subject>Cell Growth Processes - physiology</subject><subject>colon</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colorectal cancer</subject><subject>drugs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>granulocyte colony-stimulating factor</subject><subject>Granulocyte Colony-Stimulating Factor - biosynthesis</subject><subject>Granulocyte Colony-Stimulating Factor - genetics</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>messenger RNA</subject><subject>Microbiology</subject><subject>Original Research Paper</subject><subject>permeability</subject><subject>polyacrylamide gel electrophoresis</subject><subject>Polymerase chain reaction</subject><subject>protein synthesis</subject><subject>receptors</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Sensitivity and Specificity</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kl9rFDEUxYModq1-AF80IEL7MJr_mXkRZGmrUPCh9jnczWRmU2aSNZkp7rc3y6y1vhQCebi_c-6BcxF6S8knSoj-nCmRrKkIZRVptK7kM7SiUvNKaa2eoxWhglZSNOwEvcr5jpBCEf0SnTCmqWBardB48XuXXM4-Bhw7vJ1HCLhPEOYh2v3ksI1DDHucJz_OA0w-9LgDO8WEz7ZX1frm8hz7sFAYWheihWR9iCNg64YBDz44fLYGGyt2_hq96GDI7s3xP0W3lxc_19-q6x9X39dfryurqJgqTlu-oR0IsMCJsOVxpYA1BJRjtW3JBlzbagVCsG6jZc1dzSRtXe10TQU_RV8W3928GV1rXZgSDGaX_AhpbyJ48_8k-K3p473houZCk2Lw4WiQ4q_Z5cncxTmFktlQSqWijAhVKLpQNsWck-seNlBiDg2ZpSFTGjKHhowsmnePoz0o_lZSgI9HALKFoStdWJ__cYor1tADxxYul1HoXXoU8Ynt7xdRB9FAn4rx7Q0rd1JuQ0ip6ycJpjmv-R8KA7ui</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Jana, Snehasis</creator><creator>Patel, Hitesh</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20121001</creationdate><title>Expression of human granulocyte colony stimulating factor (hG-CSF) in colon adenocarcinoma cell line (Caco-2)</title><author>Jana, Snehasis ; Patel, Hitesh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c614t-31d3b1fa4aca304c04c366a290a6e28cd0baedd76a442fb7583e8251de8e78143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>adenocarcinoma</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Caco-2 Cells</topic><topic>Cell growth</topic><topic>Cell Growth Processes - physiology</topic><topic>colon</topic><topic>Colonic Neoplasms - genetics</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - pathology</topic><topic>Colorectal cancer</topic><topic>drugs</topic><topic>Fundamental and applied biological sciences. 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Lett</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>34</volume><issue>10</issue><spage>1791</spage><epage>1796</epage><pages>1791-1796</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Growth and progression of many cancer cells are mediated by alterations in the microenvironment often caused by an aberrant expression of growth factors and receptors. There is no report on expression of growth factor granulocyte colony-stimulating factor (G-CSF) in the experimental model, colon adenocarcinoma cell line (Caco2), that is commonly used in drug permeability assays. We hypothesize that in vitro, the Caco2 model is associated with a constitutive neo-expression of the hematopoietic G-CSF thereby causing an autocrine stimulation of Caco2 growth and proliferation in vitro. To test our hypothesis, we analyzed mRNA and protein expression of G-CSF in Caco2 cells using reverse transcriptase-PCR and SDS-PAGE. G-CSF mRNA and protein were detected in Caco2 cells. Expression of G-CSF protein was similar at different passages of this cell line. The expression of G-CSF has a significant role in the autocrine regulation of Caco2 cell growth and proliferation.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>22714276</pmid><doi>10.1007/s10529-012-0977-5</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biotechnology letters, 2012-10, Vol.34 (10), p.1791-1796 |
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| subjects | adenocarcinoma Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology Applied Microbiology Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Caco-2 Cells Cell growth Cell Growth Processes - physiology colon Colonic Neoplasms - genetics Colonic Neoplasms - metabolism Colonic Neoplasms - pathology Colorectal cancer drugs Fundamental and applied biological sciences. Psychology granulocyte colony-stimulating factor Granulocyte Colony-Stimulating Factor - biosynthesis Granulocyte Colony-Stimulating Factor - genetics Growth factors Humans Life Sciences messenger RNA Microbiology Original Research Paper permeability polyacrylamide gel electrophoresis Polymerase chain reaction protein synthesis receptors Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Sensitivity and Specificity |
| title | Expression of human granulocyte colony stimulating factor (hG-CSF) in colon adenocarcinoma cell line (Caco-2) |
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