Exercise Alleviates Lipid-Induced Insulin Resistance in Human Skeletal Muscle―Signaling Interaction at the Level of TBC1 Domain Family Member 4
Excess lipid availability causes insulin resistance. We examined the effect of acute exercise on lipid-induced insulin resistance and TBC1 domain family member 1/4 (TBCD1/4)-related signaling in skeletal muscle. In eight healthy young male subjects, 1 h of one-legged knee-extensor exercise was follo...
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| Veröffentlicht in: | Diabetes (New York, N.Y.) N.Y.), 2012-11, Vol.61 (11), p.2743-2752 |
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| creator | PEHMØLLER, Christian BRANDT, Nina BIRK, Jesper B HØEG, Louise D SJØBERG, Kim A GOODYEAR, Laurie J KIENS, Bente RICHTER, Erik A WOJTASZEWSKI, Jørgen F. P |
| description | Excess lipid availability causes insulin resistance. We examined the effect of acute exercise on lipid-induced insulin resistance and TBC1 domain family member 1/4 (TBCD1/4)-related signaling in skeletal muscle. In eight healthy young male subjects, 1 h of one-legged knee-extensor exercise was followed by 7 h of saline or intralipid infusion. During the last 2 h, a hyperinsulinemic-euglycemic clamp was performed. Femoral catheterization and analysis of biopsy specimens enabled measurements of leg substrate balance and muscle signaling. Each subject underwent two experimental trials, differing only by saline or intralipid infusion. Glucose infusion rate and leg glucose uptake was decreased by intralipid. Insulin-stimulated glucose uptake was higher in the prior exercised leg in the saline and the lipid trials. In the lipid trial, prior exercise normalized insulin-stimulated glucose uptake to the level observed in the resting control leg in the saline trial. Insulin increased phosphorylation of TBC1D1/4. Whereas prior exercise enhanced TBC1D4 phosphorylation on all investigated sites compared with the rested leg, intralipid impaired TBC1D4 S341 phosphorylation compared with the control trial. Intralipid enhanced pyruvate dehydrogenase (PDH) phosphorylation and lactate release. Prior exercise led to higher PDH phosphorylation and activation of glycogen synthase compared with resting control. In conclusion, lipid-induced insulin resistance in skeletal muscle was associated with impaired TBC1D4 S341 and elevated PDH phosphorylation. The prophylactic effect of exercise on lipid-induced insulin resistance may involve augmented TBC1D4 signaling and glycogen synthase activation. |
| doi_str_mv | 10.2337/db11-1572 |
| format | Article |
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P</creator><creatorcontrib>PEHMØLLER, Christian ; BRANDT, Nina ; BIRK, Jesper B ; HØEG, Louise D ; SJØBERG, Kim A ; GOODYEAR, Laurie J ; KIENS, Bente ; RICHTER, Erik A ; WOJTASZEWSKI, Jørgen F. P</creatorcontrib><description>Excess lipid availability causes insulin resistance. We examined the effect of acute exercise on lipid-induced insulin resistance and TBC1 domain family member 1/4 (TBCD1/4)-related signaling in skeletal muscle. In eight healthy young male subjects, 1 h of one-legged knee-extensor exercise was followed by 7 h of saline or intralipid infusion. During the last 2 h, a hyperinsulinemic-euglycemic clamp was performed. Femoral catheterization and analysis of biopsy specimens enabled measurements of leg substrate balance and muscle signaling. Each subject underwent two experimental trials, differing only by saline or intralipid infusion. Glucose infusion rate and leg glucose uptake was decreased by intralipid. Insulin-stimulated glucose uptake was higher in the prior exercised leg in the saline and the lipid trials. In the lipid trial, prior exercise normalized insulin-stimulated glucose uptake to the level observed in the resting control leg in the saline trial. Insulin increased phosphorylation of TBC1D1/4. Whereas prior exercise enhanced TBC1D4 phosphorylation on all investigated sites compared with the rested leg, intralipid impaired TBC1D4 S341 phosphorylation compared with the control trial. Intralipid enhanced pyruvate dehydrogenase (PDH) phosphorylation and lactate release. Prior exercise led to higher PDH phosphorylation and activation of glycogen synthase compared with resting control. In conclusion, lipid-induced insulin resistance in skeletal muscle was associated with impaired TBC1D4 S341 and elevated PDH phosphorylation. The prophylactic effect of exercise on lipid-induced insulin resistance may involve augmented TBC1D4 signaling and glycogen synthase activation.</description><identifier>ISSN: 0012-1797</identifier><identifier>EISSN: 1939-327X</identifier><identifier>DOI: 10.2337/db11-1572</identifier><identifier>PMID: 22851577</identifier><identifier>CODEN: DIAEAZ</identifier><language>eng</language><publisher>Alexandria, VA: American Diabetes Association</publisher><subject>Adult ; Analysis ; Biological and medical sciences ; Catheters ; Dehydrogenases ; Diabetes ; Diabetes. Impaired glucose tolerance ; Emulsions - adverse effects ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Exercise ; Fat Emulsions, Intravenous - adverse effects ; Fatty acids ; Fundamental and applied biological sciences. Psychology ; Glucose ; Glucose - administration & dosage ; Glucose - metabolism ; Glycogen Synthase - metabolism ; GTPase-Activating Proteins - metabolism ; Health aspects ; Humans ; Hypoglycemic Agents - pharmacology ; Hypotheses ; Insulin - pharmacology ; Insulin Resistance ; Insulin, Regular, Pork ; Kinases ; Lactic Acid - metabolism ; Leg ; Lipids ; Male ; Medical sciences ; Metabolism ; Muscle, Skeletal - metabolism ; Musculoskeletal system ; Phospholipids - adverse effects ; Phosphorylation ; Phosphorylation - drug effects ; Protein Processing, Post-Translational - drug effects ; Proteins ; Pyruvate Dehydrogenase Complex - metabolism ; Signal transduction ; Signal Transduction - drug effects ; Soybean Oil - adverse effects ; Striated muscle. Tendons ; Vertebrates: osteoarticular system, musculoskeletal system ; Workloads</subject><ispartof>Diabetes (New York, N.Y.), 2012-11, Vol.61 (11), p.2743-2752</ispartof><rights>2014 INIST-CNRS</rights><rights>COPYRIGHT 2012 American Diabetes Association</rights><rights>COPYRIGHT 2012 American Diabetes Association</rights><rights>Copyright American Diabetes Association Nov 2012</rights><rights>2012 by the American Diabetes Association. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c676t-b7d7fe09817194a6a9d2cdf94e32b2a99927d6a2034f0e9cc21b44f9548384f63</citedby><cites>FETCH-LOGICAL-c676t-b7d7fe09817194a6a9d2cdf94e32b2a99927d6a2034f0e9cc21b44f9548384f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478539/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478539/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26691102$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22851577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PEHMØLLER, Christian</creatorcontrib><creatorcontrib>BRANDT, Nina</creatorcontrib><creatorcontrib>BIRK, Jesper B</creatorcontrib><creatorcontrib>HØEG, Louise D</creatorcontrib><creatorcontrib>SJØBERG, Kim A</creatorcontrib><creatorcontrib>GOODYEAR, Laurie J</creatorcontrib><creatorcontrib>KIENS, Bente</creatorcontrib><creatorcontrib>RICHTER, Erik A</creatorcontrib><creatorcontrib>WOJTASZEWSKI, Jørgen F. P</creatorcontrib><title>Exercise Alleviates Lipid-Induced Insulin Resistance in Human Skeletal Muscle―Signaling Interaction at the Level of TBC1 Domain Family Member 4</title><title>Diabetes (New York, N.Y.)</title><addtitle>Diabetes</addtitle><description>Excess lipid availability causes insulin resistance. We examined the effect of acute exercise on lipid-induced insulin resistance and TBC1 domain family member 1/4 (TBCD1/4)-related signaling in skeletal muscle. In eight healthy young male subjects, 1 h of one-legged knee-extensor exercise was followed by 7 h of saline or intralipid infusion. During the last 2 h, a hyperinsulinemic-euglycemic clamp was performed. Femoral catheterization and analysis of biopsy specimens enabled measurements of leg substrate balance and muscle signaling. Each subject underwent two experimental trials, differing only by saline or intralipid infusion. Glucose infusion rate and leg glucose uptake was decreased by intralipid. Insulin-stimulated glucose uptake was higher in the prior exercised leg in the saline and the lipid trials. In the lipid trial, prior exercise normalized insulin-stimulated glucose uptake to the level observed in the resting control leg in the saline trial. Insulin increased phosphorylation of TBC1D1/4. Whereas prior exercise enhanced TBC1D4 phosphorylation on all investigated sites compared with the rested leg, intralipid impaired TBC1D4 S341 phosphorylation compared with the control trial. Intralipid enhanced pyruvate dehydrogenase (PDH) phosphorylation and lactate release. Prior exercise led to higher PDH phosphorylation and activation of glycogen synthase compared with resting control. In conclusion, lipid-induced insulin resistance in skeletal muscle was associated with impaired TBC1D4 S341 and elevated PDH phosphorylation. The prophylactic effect of exercise on lipid-induced insulin resistance may involve augmented TBC1D4 signaling and glycogen synthase activation.</description><subject>Adult</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Catheters</subject><subject>Dehydrogenases</subject><subject>Diabetes</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Emulsions - adverse effects</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Exercise</subject><subject>Fat Emulsions, Intravenous - adverse effects</subject><subject>Fatty acids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose</subject><subject>Glucose - administration & dosage</subject><subject>Glucose - metabolism</subject><subject>Glycogen Synthase - metabolism</subject><subject>GTPase-Activating Proteins - metabolism</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Hypoglycemic Agents - pharmacology</subject><subject>Hypotheses</subject><subject>Insulin - pharmacology</subject><subject>Insulin Resistance</subject><subject>Insulin, Regular, Pork</subject><subject>Kinases</subject><subject>Lactic Acid - metabolism</subject><subject>Leg</subject><subject>Lipids</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolism</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Musculoskeletal system</subject><subject>Phospholipids - adverse effects</subject><subject>Phosphorylation</subject><subject>Phosphorylation - drug effects</subject><subject>Protein Processing, Post-Translational - drug effects</subject><subject>Proteins</subject><subject>Pyruvate Dehydrogenase Complex - metabolism</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>Soybean Oil - adverse effects</subject><subject>Striated muscle. 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P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exercise Alleviates Lipid-Induced Insulin Resistance in Human Skeletal Muscle―Signaling Interaction at the Level of TBC1 Domain Family Member 4</atitle><jtitle>Diabetes (New York, N.Y.)</jtitle><addtitle>Diabetes</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>61</volume><issue>11</issue><spage>2743</spage><epage>2752</epage><pages>2743-2752</pages><issn>0012-1797</issn><eissn>1939-327X</eissn><coden>DIAEAZ</coden><abstract>Excess lipid availability causes insulin resistance. We examined the effect of acute exercise on lipid-induced insulin resistance and TBC1 domain family member 1/4 (TBCD1/4)-related signaling in skeletal muscle. In eight healthy young male subjects, 1 h of one-legged knee-extensor exercise was followed by 7 h of saline or intralipid infusion. During the last 2 h, a hyperinsulinemic-euglycemic clamp was performed. Femoral catheterization and analysis of biopsy specimens enabled measurements of leg substrate balance and muscle signaling. Each subject underwent two experimental trials, differing only by saline or intralipid infusion. Glucose infusion rate and leg glucose uptake was decreased by intralipid. Insulin-stimulated glucose uptake was higher in the prior exercised leg in the saline and the lipid trials. In the lipid trial, prior exercise normalized insulin-stimulated glucose uptake to the level observed in the resting control leg in the saline trial. Insulin increased phosphorylation of TBC1D1/4. Whereas prior exercise enhanced TBC1D4 phosphorylation on all investigated sites compared with the rested leg, intralipid impaired TBC1D4 S341 phosphorylation compared with the control trial. Intralipid enhanced pyruvate dehydrogenase (PDH) phosphorylation and lactate release. Prior exercise led to higher PDH phosphorylation and activation of glycogen synthase compared with resting control. In conclusion, lipid-induced insulin resistance in skeletal muscle was associated with impaired TBC1D4 S341 and elevated PDH phosphorylation. The prophylactic effect of exercise on lipid-induced insulin resistance may involve augmented TBC1D4 signaling and glycogen synthase activation.</abstract><cop>Alexandria, VA</cop><pub>American Diabetes Association</pub><pmid>22851577</pmid><doi>10.2337/db11-1572</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-1797 |
| ispartof | Diabetes (New York, N.Y.), 2012-11, Vol.61 (11), p.2743-2752 |
| issn | 0012-1797 1939-327X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3478539 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Journals@Ovid Ovid Autoload; PubMed Central |
| subjects | Adult Analysis Biological and medical sciences Catheters Dehydrogenases Diabetes Diabetes. Impaired glucose tolerance Emulsions - adverse effects Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Exercise Fat Emulsions, Intravenous - adverse effects Fatty acids Fundamental and applied biological sciences. Psychology Glucose Glucose - administration & dosage Glucose - metabolism Glycogen Synthase - metabolism GTPase-Activating Proteins - metabolism Health aspects Humans Hypoglycemic Agents - pharmacology Hypotheses Insulin - pharmacology Insulin Resistance Insulin, Regular, Pork Kinases Lactic Acid - metabolism Leg Lipids Male Medical sciences Metabolism Muscle, Skeletal - metabolism Musculoskeletal system Phospholipids - adverse effects Phosphorylation Phosphorylation - drug effects Protein Processing, Post-Translational - drug effects Proteins Pyruvate Dehydrogenase Complex - metabolism Signal transduction Signal Transduction - drug effects Soybean Oil - adverse effects Striated muscle. Tendons Vertebrates: osteoarticular system, musculoskeletal system Workloads |
| title | Exercise Alleviates Lipid-Induced Insulin Resistance in Human Skeletal Muscle―Signaling Interaction at the Level of TBC1 Domain Family Member 4 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T15%3A51%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Exercise%20Alleviates%20Lipid-Induced%20Insulin%20Resistance%20in%20Human%20Skeletal%20Muscle%E2%80%95Signaling%20Interaction%20at%20the%20Level%20of%20TBC1%20Domain%20Family%20Member%204&rft.jtitle=Diabetes%20(New%20York,%20N.Y.)&rft.au=PEHM%C3%98LLER,%20Christian&rft.date=2012-11-01&rft.volume=61&rft.issue=11&rft.spage=2743&rft.epage=2752&rft.pages=2743-2752&rft.issn=0012-1797&rft.eissn=1939-327X&rft.coden=DIAEAZ&rft_id=info:doi/10.2337/db11-1572&rft_dat=%3Cgale_pubme%3EA311048410%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1268715474&rft_id=info:pmid/22851577&rft_galeid=A311048410&rfr_iscdi=true |