Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

The enzyme-linked immunosorbent assay (ELISA) has long been the primary tool for detection of analytes of interest in biological samples for both life science research and clinical diagnostics. However, ELISA has limitations. It is typically performed in a 96-well microplate, and the wells are coate...

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Veröffentlicht in:	Journal of Visualized Experiments 2012-07 (65)
Hauptverfasser:	Baker, Harold N., Murphy, Robin, Lopez, Erica, Garcia, Carlos
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies - analysis Antibodies - chemistry Antibodies - immunology Antibody Specificity Enzyme-Linked Immunosorbent Assay - instrumentation Enzyme-Linked Immunosorbent Assay - methods Humans Microspheres Molecular Biology Reagent Kits, Diagnostic Tumor Necrosis Factor-alpha - analysis Tumor Necrosis Factor-alpha - immunology
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