Evidence for apoptosis, MMP-1, MMP-3 and TIMP-2 expression and their effect on the mechanical and biochemical properties of fresh viable knee medial meniscal allografts and autografts in the rabbit

Evidence for apoptosis, MMP-1, MMP-3 and TIMP-2 expression and their effect on the mechanical and biochemical properties of fresh viable knee medial meniscal allografts and autografts in the rabbit

The study sought evidence for apoptosis, the expression of MMP-1, MMP-3 and TIMP-2 and their effect on the mechanical and biochemical properties of rabbit fresh knee medial meniscal grafts in a 6-month follow-up. Forty white male New Zealand rabbits were chosen for the study. The medial meniscus was...

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Veröffentlicht in:Archives of medical science 2012-09, Vol.8 (4), p.724-732
Hauptverfasser: Zwierzchowski, Tomasz J, Stasikowska-Kanicka, Olga, Janus, Jolanta, Konecki, Włodzimierz, Danilewicz, Marian, Fabiś, Jarosław
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container_issue 4
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container_title Archives of medical science
container_volume 8
creator Zwierzchowski, Tomasz J
Stasikowska-Kanicka, Olga
Janus, Jolanta
Konecki, Włodzimierz
Danilewicz, Marian
Fabiś, Jarosław
description The study sought evidence for apoptosis, the expression of MMP-1, MMP-3 and TIMP-2 and their effect on the mechanical and biochemical properties of rabbit fresh knee medial meniscal grafts in a 6-month follow-up. Forty white male New Zealand rabbits were chosen for the study. The medial meniscus was excised from 28 animals and stored under tissue culture conditions for 2 weeks, following which 14 of them were implanted as autografts and 14 as allografts. When the animals were euthanized, 20 menisci were used for immunohistochemical examinations. Apoptosis (TUNEL method) and MMP-1, MMP-3 and TIMP-2 immunoexpression were estimated semiquantitatively. The other 20 menisci were subjected to biochemical analysis and their degree of elasticity was evaluated. An increased level of apoptosis (p
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Forty white male New Zealand rabbits were chosen for the study. The medial meniscus was excised from 28 animals and stored under tissue culture conditions for 2 weeks, following which 14 of them were implanted as autografts and 14 as allografts. When the animals were euthanized, 20 menisci were used for immunohistochemical examinations. Apoptosis (TUNEL method) and MMP-1, MMP-3 and TIMP-2 immunoexpression were estimated semiquantitatively. The other 20 menisci were subjected to biochemical analysis and their degree of elasticity was evaluated. An increased level of apoptosis (p &lt;0.05) was observed both in allografts (1.57 ±0.98) and autografts (0.86 ±0.69); no statistical differences existed between them. An increased level of metalloproteinases and TIMP-2 expression was observed only in the allografts (p &lt; 0.05). The highest decrease of degree of elasticity and the most significant changes in biochemical composition were observed in allografts (p &lt; 0.05). THE STUDIES CONFIRMED THE EXISTENCE OF EXCESSIVE APOPTOSIS IN BOTH KINDS OF FRESH VIABLE MEDIAL MENISCAL IMPLANTS: auto- and allografts. Our results suggest that apoptosis and increased MMP-1 and MMP-3 expression have an adverse effect on the biological properties of implants. 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Forty white male New Zealand rabbits were chosen for the study. The medial meniscus was excised from 28 animals and stored under tissue culture conditions for 2 weeks, following which 14 of them were implanted as autografts and 14 as allografts. When the animals were euthanized, 20 menisci were used for immunohistochemical examinations. Apoptosis (TUNEL method) and MMP-1, MMP-3 and TIMP-2 immunoexpression were estimated semiquantitatively. The other 20 menisci were subjected to biochemical analysis and their degree of elasticity was evaluated. An increased level of apoptosis (p &lt;0.05) was observed both in allografts (1.57 ±0.98) and autografts (0.86 ±0.69); no statistical differences existed between them. An increased level of metalloproteinases and TIMP-2 expression was observed only in the allografts (p &lt; 0.05). The highest decrease of degree of elasticity and the most significant changes in biochemical composition were observed in allografts (p &lt; 0.05). THE STUDIES CONFIRMED THE EXISTENCE OF EXCESSIVE APOPTOSIS IN BOTH KINDS OF FRESH VIABLE MEDIAL MENISCAL IMPLANTS: auto- and allografts. Our results suggest that apoptosis and increased MMP-1 and MMP-3 expression have an adverse effect on the biological properties of implants. 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title Evidence for apoptosis, MMP-1, MMP-3 and TIMP-2 expression and their effect on the mechanical and biochemical properties of fresh viable knee medial meniscal allografts and autografts in the rabbit
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