Objective assessments of temperature maintenance using in vitro culture techniques
To assess the ability of various facets of embryo culture (microscope stage warmers, volumes of culture media, culture vessel lids, and type of culture incubator) to maintain a constant temperature in vitro. Ability to maintain 37.0 degrees C in the microenvironment of gametes was recorded by digita...
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| Veröffentlicht in: | Journal of assisted reproduction and genetics 2002-08, Vol.19 (8), p.368-375 |
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| container_title | Journal of assisted reproduction and genetics |
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| creator | COOKE, Simon TYLER, John P. P DRISCOLL, Geoff |
| description | To assess the ability of various facets of embryo culture (microscope stage warmers, volumes of culture media, culture vessel lids, and type of culture incubator) to maintain a constant temperature in vitro.
Ability to maintain 37.0 degrees C in the microenvironment of gametes was recorded by digital thermocouple in the chosen facets of in vitro culture.
Stage warmers are highly variable in their ability to maintain the set temperature (range 33.8 degrees C-37.0 degrees C after 60 s). Temperature loss in culture media is both volume and vessel dependent, and the direct heat transfer culture incubator (MINC) has superior temperature maintenance compared with a large volume air convection incubator (FORMA), where temperature regain from 35.0 degrees C to 37.0 degrees C took 5.5 min compared to >20 min.
There are large measurable differences in the ability to maintain set temperature that depend on the stage warmer used, volume of media, use of vessel lids, and the type of incubator chosen for IVF culture. |
| doi_str_mv | 10.1023/A:1016394304339 |
| format | Article |
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Ability to maintain 37.0 degrees C in the microenvironment of gametes was recorded by digital thermocouple in the chosen facets of in vitro culture.
Stage warmers are highly variable in their ability to maintain the set temperature (range 33.8 degrees C-37.0 degrees C after 60 s). Temperature loss in culture media is both volume and vessel dependent, and the direct heat transfer culture incubator (MINC) has superior temperature maintenance compared with a large volume air convection incubator (FORMA), where temperature regain from 35.0 degrees C to 37.0 degrees C took 5.5 min compared to >20 min.
There are large measurable differences in the ability to maintain set temperature that depend on the stage warmer used, volume of media, use of vessel lids, and the type of incubator chosen for IVF culture.</description><identifier>ISSN: 1058-0468</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1023/A:1016394304339</identifier><identifier>PMID: 12182443</identifier><identifier>CODEN: JARGE4</identifier><language>eng</language><publisher>New York, NY: Kluwer/Plenum</publisher><subject>Biological and medical sciences ; Cell Culture Techniques - instrumentation ; Cell Culture Techniques - methods ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic and Fetal Development - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects. Development. Fetal membranes ; Humans ; Incubators - standards ; Male ; Reproductive Techniques, Assisted - instrumentation ; Temperature</subject><ispartof>Journal of assisted reproduction and genetics, 2002-08, Vol.19 (8), p.368-375</ispartof><rights>2002 INIST-CNRS</rights><rights>Plenum Publishing Corporation 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-5e8c876e45d126664a3041d77c4f01f2abf3aa1859e366934943885e3653dee43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3455576/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3455576/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13856122$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12182443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>COOKE, Simon</creatorcontrib><creatorcontrib>TYLER, John P. P</creatorcontrib><creatorcontrib>DRISCOLL, Geoff</creatorcontrib><title>Objective assessments of temperature maintenance using in vitro culture techniques</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><description>To assess the ability of various facets of embryo culture (microscope stage warmers, volumes of culture media, culture vessel lids, and type of culture incubator) to maintain a constant temperature in vitro.
Ability to maintain 37.0 degrees C in the microenvironment of gametes was recorded by digital thermocouple in the chosen facets of in vitro culture.
Stage warmers are highly variable in their ability to maintain the set temperature (range 33.8 degrees C-37.0 degrees C after 60 s). Temperature loss in culture media is both volume and vessel dependent, and the direct heat transfer culture incubator (MINC) has superior temperature maintenance compared with a large volume air convection incubator (FORMA), where temperature regain from 35.0 degrees C to 37.0 degrees C took 5.5 min compared to >20 min.
There are large measurable differences in the ability to maintain set temperature that depend on the stage warmer used, volume of media, use of vessel lids, and the type of incubator chosen for IVF culture.</description><subject>Biological and medical sciences</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Culture Techniques - methods</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic and Fetal Development - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects. Development. Fetal membranes</subject><subject>Humans</subject><subject>Incubators - standards</subject><subject>Male</subject><subject>Reproductive Techniques, Assisted - instrumentation</subject><subject>Temperature</subject><issn>1058-0468</issn><issn>1573-7330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUEtLAzEQDqJYrZ69yV70tprsJNmsB6EUX1AoiJ6XNJ1tU3azdZMt-O9Ntb5OM8N8fC9Czhi9YjSD69ENo0xCwYFygGKPHDGRQ5oD0P24U6FSyqUakGPvV5TSQmVwSAYsYyrjHI7I83S2QhPsBhPtPXrfoAs-aaskYLPGToe-w6TR1gV02hlMem_dIrEu2djQtYnp609IQLN09q1Hf0IOKl17PN3NIXm9v3sZP6aT6cPTeDRJDeRFSAUqo3KJXMxZJqXkOmZg8zw3vKKsyvSsAq2ZEgWClAXwGFIpEQ8Bc0QOQ3L7xbvuZw3OTTTe6bpcd7bR3XvZalv-_zi7LBftpgQuhMhlJLjcEXTt1ngoG-sN1rV22Pa-zFlRcC63Sud_lX4kvmuMgIsdQHuj66qLTVn_iwMlJMsy-ADyG4Mi</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>COOKE, Simon</creator><creator>TYLER, John P. P</creator><creator>DRISCOLL, Geoff</creator><general>Kluwer/Plenum</general><general>Kluwer Academic Publishers-Plenum Publishers</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020801</creationdate><title>Objective assessments of temperature maintenance using in vitro culture techniques</title><author>COOKE, Simon ; TYLER, John P. P ; DRISCOLL, Geoff</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-5e8c876e45d126664a3041d77c4f01f2abf3aa1859e366934943885e3653dee43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Cell Culture Techniques - methods</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic and Fetal Development - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects. Development. Fetal membranes</topic><topic>Humans</topic><topic>Incubators - standards</topic><topic>Male</topic><topic>Reproductive Techniques, Assisted - instrumentation</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>COOKE, Simon</creatorcontrib><creatorcontrib>TYLER, John P. P</creatorcontrib><creatorcontrib>DRISCOLL, Geoff</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>COOKE, Simon</au><au>TYLER, John P. P</au><au>DRISCOLL, Geoff</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Objective assessments of temperature maintenance using in vitro culture techniques</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><addtitle>J Assist Reprod Genet</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>19</volume><issue>8</issue><spage>368</spage><epage>375</epage><pages>368-375</pages><issn>1058-0468</issn><eissn>1573-7330</eissn><coden>JARGE4</coden><abstract>To assess the ability of various facets of embryo culture (microscope stage warmers, volumes of culture media, culture vessel lids, and type of culture incubator) to maintain a constant temperature in vitro.
Ability to maintain 37.0 degrees C in the microenvironment of gametes was recorded by digital thermocouple in the chosen facets of in vitro culture.
Stage warmers are highly variable in their ability to maintain the set temperature (range 33.8 degrees C-37.0 degrees C after 60 s). Temperature loss in culture media is both volume and vessel dependent, and the direct heat transfer culture incubator (MINC) has superior temperature maintenance compared with a large volume air convection incubator (FORMA), where temperature regain from 35.0 degrees C to 37.0 degrees C took 5.5 min compared to >20 min.
There are large measurable differences in the ability to maintain set temperature that depend on the stage warmer used, volume of media, use of vessel lids, and the type of incubator chosen for IVF culture.</abstract><cop>New York, NY</cop><pub>Kluwer/Plenum</pub><pmid>12182443</pmid><doi>10.1023/A:1016394304339</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of assisted reproduction and genetics, 2002-08, Vol.19 (8), p.368-375 |
| issn | 1058-0468 1573-7330 |
| language | eng |
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| source | MEDLINE; Springer Nature; PubMed Central |
| subjects | Biological and medical sciences Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Embryology: invertebrates and vertebrates. Teratology Embryonic and Fetal Development - physiology Female Fundamental and applied biological sciences. Psychology General aspects General aspects. Development. Fetal membranes Humans Incubators - standards Male Reproductive Techniques, Assisted - instrumentation Temperature |
| title | Objective assessments of temperature maintenance using in vitro culture techniques |
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