Analysis of the human zona pellucida during culture : Correlation with diagnosis and the preovulatory hormonal environment
The objective of this study was to analyze sequentially the human zona pellucida changes in an in vitro fertilization program as it relates to several variables. The zona pellucida thickness was measured daily in zygotes and cleavage-stage embryos on a Nikon inverted microscope equipped with Hoffman...
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| Veröffentlicht in: | Journal of assisted reproduction and genetics 1997-07, Vol.14 (6), p.332-336 |
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| creator | LORET DE MOLA, J. R GARSIDE, W. T BUCCI, J TURECK, R. W HEYNER, S |
| description | The objective of this study was to analyze sequentially the human zona pellucida changes in an in vitro fertilization program as it relates to several variables.
The zona pellucida thickness was measured daily in zygotes and cleavage-stage embryos on a Nikon inverted microscope equipped with Hoffman modulation contrast optics, using an ocular micrometer. A total of 512 embryos from 96 patients was evaluated.
There was a highly significant direct correlation between zona thickness and preovulatory estradiol and basal day 3 FSH levels (P < 0.02 and P < 0.0006, respectively). This relationship showed a rapid reversal following 48 hr of culture; embryos from patients with the highest FSH levels had thinner zonae prior to transfer (P < 0.0007). The zonae from patients with unexplained infertility were thicker (19.4 +/- 2.7 microns) than those from patients with endometriosis (17.7 +/- 2.2 microns), tubal (17.5 +/- 2.4 microns), or male-factor infertility (16.4 +/- 2.7 microns) (P < 0.0001) on the first day of culture.
We hypothesize that the thickness of the human zona pellucida is influenced by the preovulatory hormonal environment and diagnosis. These factors should be considered as part of the embryo quality evaluation prior to transfer or when assessing the possibility of using assisted hatching. More studies are needed to understand the factors regulating the thickness of the human zona pellucida. |
| doi_str_mv | 10.1007/bf02765837 |
| format | Article |
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The zona pellucida thickness was measured daily in zygotes and cleavage-stage embryos on a Nikon inverted microscope equipped with Hoffman modulation contrast optics, using an ocular micrometer. A total of 512 embryos from 96 patients was evaluated.
There was a highly significant direct correlation between zona thickness and preovulatory estradiol and basal day 3 FSH levels (P < 0.02 and P < 0.0006, respectively). This relationship showed a rapid reversal following 48 hr of culture; embryos from patients with the highest FSH levels had thinner zonae prior to transfer (P < 0.0007). The zonae from patients with unexplained infertility were thicker (19.4 +/- 2.7 microns) than those from patients with endometriosis (17.7 +/- 2.2 microns), tubal (17.5 +/- 2.4 microns), or male-factor infertility (16.4 +/- 2.7 microns) (P < 0.0001) on the first day of culture.
We hypothesize that the thickness of the human zona pellucida is influenced by the preovulatory hormonal environment and diagnosis. These factors should be considered as part of the embryo quality evaluation prior to transfer or when assessing the possibility of using assisted hatching. More studies are needed to understand the factors regulating the thickness of the human zona pellucida.</description><identifier>ISSN: 1058-0468</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1007/bf02765837</identifier><identifier>PMID: 9226512</identifier><identifier>CODEN: JARGE4</identifier><language>eng</language><publisher>New York, NY: Kluwer/Plenum</publisher><subject>Adult ; Biological and medical sciences ; Birth control ; Blastocyst - ultrastructure ; Cells, Cultured ; Clinical Assisted Reproduction ; Embryo Transfer ; Estradiol - blood ; Female ; Fertilization in Vitro ; Follicle Stimulating Hormone - blood ; Gynecology. Andrology. Obstetrics ; Humans ; Infertility, Female - blood ; Infertility, Female - therapy ; Infertility, Male ; Male ; Medical sciences ; Observer Variation ; Ovulation - blood ; Pregnancy ; Pregnancy Rate ; Sterility. Assisted procreation ; Zona Pellucida - ultrastructure ; Zygote - ultrastructure</subject><ispartof>Journal of assisted reproduction and genetics, 1997-07, Vol.14 (6), p.332-336</ispartof><rights>1997 INIST-CNRS</rights><rights>Plenum Publishing Corporation 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-96b58ce4a1a6507b1e72c68ee03f1b2fb0be9a2ce73874f3222ed8097a3681413</citedby><cites>FETCH-LOGICAL-c402t-96b58ce4a1a6507b1e72c68ee03f1b2fb0be9a2ce73874f3222ed8097a3681413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454791/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454791/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2771671$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9226512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LORET DE MOLA, J. R</creatorcontrib><creatorcontrib>GARSIDE, W. T</creatorcontrib><creatorcontrib>BUCCI, J</creatorcontrib><creatorcontrib>TURECK, R. W</creatorcontrib><creatorcontrib>HEYNER, S</creatorcontrib><title>Analysis of the human zona pellucida during culture : Correlation with diagnosis and the preovulatory hormonal environment</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><description>The objective of this study was to analyze sequentially the human zona pellucida changes in an in vitro fertilization program as it relates to several variables.
The zona pellucida thickness was measured daily in zygotes and cleavage-stage embryos on a Nikon inverted microscope equipped with Hoffman modulation contrast optics, using an ocular micrometer. A total of 512 embryos from 96 patients was evaluated.
There was a highly significant direct correlation between zona thickness and preovulatory estradiol and basal day 3 FSH levels (P < 0.02 and P < 0.0006, respectively). This relationship showed a rapid reversal following 48 hr of culture; embryos from patients with the highest FSH levels had thinner zonae prior to transfer (P < 0.0007). The zonae from patients with unexplained infertility were thicker (19.4 +/- 2.7 microns) than those from patients with endometriosis (17.7 +/- 2.2 microns), tubal (17.5 +/- 2.4 microns), or male-factor infertility (16.4 +/- 2.7 microns) (P < 0.0001) on the first day of culture.
We hypothesize that the thickness of the human zona pellucida is influenced by the preovulatory hormonal environment and diagnosis. These factors should be considered as part of the embryo quality evaluation prior to transfer or when assessing the possibility of using assisted hatching. More studies are needed to understand the factors regulating the thickness of the human zona pellucida.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Blastocyst - ultrastructure</subject><subject>Cells, Cultured</subject><subject>Clinical Assisted Reproduction</subject><subject>Embryo Transfer</subject><subject>Estradiol - blood</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Follicle Stimulating Hormone - blood</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Infertility, Female - blood</subject><subject>Infertility, Female - therapy</subject><subject>Infertility, Male</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Observer Variation</subject><subject>Ovulation - blood</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Sterility. Assisted procreation</subject><subject>Zona Pellucida - ultrastructure</subject><subject>Zygote - ultrastructure</subject><issn>1058-0468</issn><issn>1573-7330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc-L1DAUx4Mo67p68S7kIB6Ean60TetBWAdXhQUveg6v6es0kiZj0ozM_vVm3HHQ0wt8P3zeI19CnnP2hjOm3g4TE6ptOqkekEveKFkpKdnD8mZNV7G67R6TJyn9YIz1nZAX5KIXom24uCR31x7cIdlEw0TXGemcF_D0LnigO3QuGzsCHXO0fktNdmuOSN_RTYgRHaw2ePrLrjMdLWx9OHrAj39Eu4hhnwsT4oHOIS5F6Sj6vY3BL-jXp-TRBC7hs9O8It9vPn7bfK5uv376srm-rUzNxFr17dB0Bmvg0DZMDRyVMG2HyOTEBzENbMAehEElO1VPUgiBY8d6BbLteM3lFXl_793lYcHRlNURnN5Fu0A86ABW_594O-tt2GtZN7Xqj4JXJ0EMPzOmVS82mfI54DHkpAtTq5rJAr6-B00MKUWczks408em9Iebv00V-MW_Z53RUzUlf3nKIRlwUwRvbDpjQineKi5_A2otnls</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>LORET DE MOLA, J. R</creator><creator>GARSIDE, W. T</creator><creator>BUCCI, J</creator><creator>TURECK, R. W</creator><creator>HEYNER, S</creator><general>Kluwer/Plenum</general><general>Springer US</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970701</creationdate><title>Analysis of the human zona pellucida during culture : Correlation with diagnosis and the preovulatory hormonal environment</title><author>LORET DE MOLA, J. R ; GARSIDE, W. T ; BUCCI, J ; TURECK, R. W ; HEYNER, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-96b58ce4a1a6507b1e72c68ee03f1b2fb0be9a2ce73874f3222ed8097a3681413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Blastocyst - ultrastructure</topic><topic>Cells, Cultured</topic><topic>Clinical Assisted Reproduction</topic><topic>Embryo Transfer</topic><topic>Estradiol - blood</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Follicle Stimulating Hormone - blood</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Infertility, Female - blood</topic><topic>Infertility, Female - therapy</topic><topic>Infertility, Male</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Observer Variation</topic><topic>Ovulation - blood</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Sterility. Assisted procreation</topic><topic>Zona Pellucida - ultrastructure</topic><topic>Zygote - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LORET DE MOLA, J. R</creatorcontrib><creatorcontrib>GARSIDE, W. T</creatorcontrib><creatorcontrib>BUCCI, J</creatorcontrib><creatorcontrib>TURECK, R. W</creatorcontrib><creatorcontrib>HEYNER, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LORET DE MOLA, J. R</au><au>GARSIDE, W. T</au><au>BUCCI, J</au><au>TURECK, R. W</au><au>HEYNER, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the human zona pellucida during culture : Correlation with diagnosis and the preovulatory hormonal environment</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><addtitle>J Assist Reprod Genet</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>14</volume><issue>6</issue><spage>332</spage><epage>336</epage><pages>332-336</pages><issn>1058-0468</issn><eissn>1573-7330</eissn><coden>JARGE4</coden><abstract>The objective of this study was to analyze sequentially the human zona pellucida changes in an in vitro fertilization program as it relates to several variables.
The zona pellucida thickness was measured daily in zygotes and cleavage-stage embryos on a Nikon inverted microscope equipped with Hoffman modulation contrast optics, using an ocular micrometer. A total of 512 embryos from 96 patients was evaluated.
There was a highly significant direct correlation between zona thickness and preovulatory estradiol and basal day 3 FSH levels (P < 0.02 and P < 0.0006, respectively). This relationship showed a rapid reversal following 48 hr of culture; embryos from patients with the highest FSH levels had thinner zonae prior to transfer (P < 0.0007). The zonae from patients with unexplained infertility were thicker (19.4 +/- 2.7 microns) than those from patients with endometriosis (17.7 +/- 2.2 microns), tubal (17.5 +/- 2.4 microns), or male-factor infertility (16.4 +/- 2.7 microns) (P < 0.0001) on the first day of culture.
We hypothesize that the thickness of the human zona pellucida is influenced by the preovulatory hormonal environment and diagnosis. These factors should be considered as part of the embryo quality evaluation prior to transfer or when assessing the possibility of using assisted hatching. More studies are needed to understand the factors regulating the thickness of the human zona pellucida.</abstract><cop>New York, NY</cop><pub>Kluwer/Plenum</pub><pmid>9226512</pmid><doi>10.1007/bf02765837</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of assisted reproduction and genetics, 1997-07, Vol.14 (6), p.332-336 |
| issn | 1058-0468 1573-7330 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals; PubMed Central |
| subjects | Adult Biological and medical sciences Birth control Blastocyst - ultrastructure Cells, Cultured Clinical Assisted Reproduction Embryo Transfer Estradiol - blood Female Fertilization in Vitro Follicle Stimulating Hormone - blood Gynecology. Andrology. Obstetrics Humans Infertility, Female - blood Infertility, Female - therapy Infertility, Male Male Medical sciences Observer Variation Ovulation - blood Pregnancy Pregnancy Rate Sterility. Assisted procreation Zona Pellucida - ultrastructure Zygote - ultrastructure |
| title | Analysis of the human zona pellucida during culture : Correlation with diagnosis and the preovulatory hormonal environment |
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