Cell culture derived AgMNPV bioinsecticide : biological constraints and bioprocess issues

We have studied parameters for optimizing the Spodoptera frugiperda (Sf9) cell culture and viral infection for the production of Anticarsia gemmatalis multiple nucleopolyhedrosis virus (AgMNPV) polyhedra inclusion bodies (PIBs) in shaker-Schott or spinner bottles and bioreactors. We have assayed the...

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Veröffentlicht in:	Cytotechnology (Dordrecht) 2005-06, Vol.48 (1-3), p.27-39
Hauptverfasser:	RODAS, Valeria M, MARQUES, Fabiano H, TONSO, Aldo, PEREIRA, Carlos A, HONDA, Marcelo T, SOARES, Daniela M, JORGE, Soraia A. C, ANTONIAZZI, Marta M, MEDUGNO, Claudia, CASTRO, Maria E. B, RIBEIRO, Bergmann M, SOUZA, Marlinda L
Format:	Artikel
Sprache:	eng
Schlagworte:	Anticarsia gemmatalis Biological and medical sciences Biological control Bioreactors Cell culture Cell density Dissolved oxygen Fundamental and applied biological sciences. Psychology Glutamine Hemolymph Inclusion bodies Infections Multiplicity of infection Noctuidae Polyhedra Spodoptera frugiperda Transmission electron microscopy Viral infections Virions Virulence Viruses
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