Primary adipocyte culture : adipocyte purification methods may lead to a new understanding of adipose tissue growth and development
In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2004-10, Vol.46 (2-3), p.163-172 |
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| container_title | Cytotechnology (Dordrecht) |
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| creator | FERNYHOUGH, M. E VIERCK, J. L HAUSMAN, G. J MIR, P. S OKINE, E. K DODSON, M. V |
| description | In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to reevaluate differences between presently available cellular models for lipogenesis/lipolysis and might provide a new cellular physiological system for studies utilizing the proliferative progeny from mature adipocyte dedifferentiation. Alternative possibilities to the dedifferentiation phenomenon are proposed, as this new area of research is novel. |
| doi_str_mv | 10.1007/s10616-005-2602-0 |
| format | Article |
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| ispartof | Cytotechnology (Dordrecht), 2004-10, Vol.46 (2-3), p.163-172 |
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| language | eng |
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| source | ProQuest Central UK/Ireland; PubMed Central; SpringerLink Journals - AutoHoldings; ProQuest Central |
| subjects | Adipocytes Adipose tissue Biological and medical sciences Cell culture Fundamental and applied biological sciences. Psychology Lipogenesis Lipolysis |
| title | Primary adipocyte culture : adipocyte purification methods may lead to a new understanding of adipose tissue growth and development |
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