Release of Cyclic Phosphatidic Acid from Gelatin-based Hydrogels Inhibit Colon Cancer Cell Growth and Migration
Microparticle and nanoparticle formulations are widely used to improve the bioavailability of low-solubility drugs and as vehicles for organ- and tissue-specific targeted drug delivery. We investigated the effect of a novel, controlled-release form of a bioactive lipid, cyclic phosphatidic acid (cPA...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2012-09, Vol.2 (1), p.687, Article 687 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | 687 |
| container_title | Scientific reports |
| container_volume | 2 |
| creator | Tsukahara, Tamotsu Murakami-Murofushi, Kimiko |
| description | Microparticle and nanoparticle formulations are widely used to improve the bioavailability of low-solubility drugs and as vehicles for organ- and tissue-specific targeted drug delivery. We investigated the effect of a novel, controlled-release form of a bioactive lipid, cyclic phosphatidic acid (cPA), on human colon cancer cell line functions. We encapsulated cPA in gelatin-based hydrogels and examined its ability to inhibit the viability and migration of HT-29 and DLD-1 cells
in vitro
and the LPA-induced activity of the transcription factor peroxisome proliferator-activated receptor gamma (PPARγ). The hydrogel delivery system prolonged cPA release into the culture medium. Accordingly, cPA-hydrogel microspheres substantially inhibited LPA-induced PPARγ activity and cell growth and migration compared with that of cells cultured with cPA alone. Thus, hydrogel microspheres are a potential system for stable and efficient delivery of bioactive lipids such as cPA and may offer a new strategy for targeted colon cancer treatment. |
| doi_str_mv | 10.1038/srep00687 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3449289</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1897397230</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-c97911b5e1f6022e7c3e4ecb1d1f94be1c14966475e64349feccc435bef7c4833</originalsourceid><addsrcrecordid>eNplkU1LJDEQhoOsrIN62D8ggT2t0Jqv_shlQZp1FEYU0XPoTld3RzLJbNLjMv_eyOgwsrlUknrqraJehH5QckEJry5jgBUhRVUeoBkjIs8YZ-zb3v0Incb4QtLJmRRUfkdHjBNSlTmbIf8IFpoI2Pe43mhrNH4YfVyNzWS69LjSpsN98Es8B5v-XNYmusM3my74AWzEt240rZlw7a13uG6choBrsBbPg_83jbhxHb4zQ0jV3p2gw76xEU4_4jF6vv7zVN9ki_v5bX21yHROxJRpWUpK2xxoXxDGoNQcBOiWdrSXogWqqZBFIcocCsGF7EFrLXjeQl9qUXF-jH5vdVfrdgmdBjeFxqpVMMsmbJRvjPqacWZUg39VXAjJKpkEfn4IBP93DXFSL34dXJpZ0UqWXJZpiYn6taV08DE50e86UKLe7VE7exJ7tj_Sjvw0IwHnWyCmlBsg7LX8T-0N54iaiw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1897397230</pqid></control><display><type>article</type><title>Release of Cyclic Phosphatidic Acid from Gelatin-based Hydrogels Inhibit Colon Cancer Cell Growth and Migration</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Tsukahara, Tamotsu ; Murakami-Murofushi, Kimiko</creator><creatorcontrib>Tsukahara, Tamotsu ; Murakami-Murofushi, Kimiko</creatorcontrib><description>Microparticle and nanoparticle formulations are widely used to improve the bioavailability of low-solubility drugs and as vehicles for organ- and tissue-specific targeted drug delivery. We investigated the effect of a novel, controlled-release form of a bioactive lipid, cyclic phosphatidic acid (cPA), on human colon cancer cell line functions. We encapsulated cPA in gelatin-based hydrogels and examined its ability to inhibit the viability and migration of HT-29 and DLD-1 cells
in vitro
and the LPA-induced activity of the transcription factor peroxisome proliferator-activated receptor gamma (PPARγ). The hydrogel delivery system prolonged cPA release into the culture medium. Accordingly, cPA-hydrogel microspheres substantially inhibited LPA-induced PPARγ activity and cell growth and migration compared with that of cells cultured with cPA alone. Thus, hydrogel microspheres are a potential system for stable and efficient delivery of bioactive lipids such as cPA and may offer a new strategy for targeted colon cancer treatment.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep00687</identifier><identifier>PMID: 23008752</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/154/555 ; 631/337/641/83 ; 631/45/287 ; 631/67/70 ; Bioavailability ; Cell culture ; Cell growth ; Cell Movement - drug effects ; Cell Proliferation - drug effects ; Colon cancer ; Colonic Neoplasms - drug therapy ; Colorectal cancer ; Controlled release ; Drug delivery ; Drug Delivery Systems ; Gelatin ; Gelatin - chemistry ; Gelatin - pharmacology ; HT29 Cells ; Humanities and Social Sciences ; Humans ; Hydrogels ; Hydrogels - chemistry ; Hydrogels - pharmacology ; Lipids ; Lysophospholipids ; Microspheres ; multidisciplinary ; Nanoparticles ; Nanoparticles - chemistry ; Phosphatidic acid ; Phosphatidic Acids - chemistry ; Phosphatidic Acids - pharmacology ; PPAR gamma - chemistry ; PPAR gamma - genetics ; Science</subject><ispartof>Scientific reports, 2012-09, Vol.2 (1), p.687, Article 687</ispartof><rights>The Author(s) 2012</rights><rights>Copyright Nature Publishing Group Sep 2012</rights><rights>Copyright © 2012, Macmillan Publishers Limited. All rights reserved 2012 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-c97911b5e1f6022e7c3e4ecb1d1f94be1c14966475e64349feccc435bef7c4833</citedby><cites>FETCH-LOGICAL-c504t-c97911b5e1f6022e7c3e4ecb1d1f94be1c14966475e64349feccc435bef7c4833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449289/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449289/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23008752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsukahara, Tamotsu</creatorcontrib><creatorcontrib>Murakami-Murofushi, Kimiko</creatorcontrib><title>Release of Cyclic Phosphatidic Acid from Gelatin-based Hydrogels Inhibit Colon Cancer Cell Growth and Migration</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Microparticle and nanoparticle formulations are widely used to improve the bioavailability of low-solubility drugs and as vehicles for organ- and tissue-specific targeted drug delivery. We investigated the effect of a novel, controlled-release form of a bioactive lipid, cyclic phosphatidic acid (cPA), on human colon cancer cell line functions. We encapsulated cPA in gelatin-based hydrogels and examined its ability to inhibit the viability and migration of HT-29 and DLD-1 cells
in vitro
and the LPA-induced activity of the transcription factor peroxisome proliferator-activated receptor gamma (PPARγ). The hydrogel delivery system prolonged cPA release into the culture medium. Accordingly, cPA-hydrogel microspheres substantially inhibited LPA-induced PPARγ activity and cell growth and migration compared with that of cells cultured with cPA alone. Thus, hydrogel microspheres are a potential system for stable and efficient delivery of bioactive lipids such as cPA and may offer a new strategy for targeted colon cancer treatment.</description><subject>631/154/555</subject><subject>631/337/641/83</subject><subject>631/45/287</subject><subject>631/67/70</subject><subject>Bioavailability</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colorectal cancer</subject><subject>Controlled release</subject><subject>Drug delivery</subject><subject>Drug Delivery Systems</subject><subject>Gelatin</subject><subject>Gelatin - chemistry</subject><subject>Gelatin - pharmacology</subject><subject>HT29 Cells</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogels - pharmacology</subject><subject>Lipids</subject><subject>Lysophospholipids</subject><subject>Microspheres</subject><subject>multidisciplinary</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Phosphatidic acid</subject><subject>Phosphatidic Acids - chemistry</subject><subject>Phosphatidic Acids - pharmacology</subject><subject>PPAR gamma - chemistry</subject><subject>PPAR gamma - genetics</subject><subject>Science</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkU1LJDEQhoOsrIN62D8ggT2t0Jqv_shlQZp1FEYU0XPoTld3RzLJbNLjMv_eyOgwsrlUknrqraJehH5QckEJry5jgBUhRVUeoBkjIs8YZ-zb3v0Incb4QtLJmRRUfkdHjBNSlTmbIf8IFpoI2Pe43mhrNH4YfVyNzWS69LjSpsN98Es8B5v-XNYmusM3my74AWzEt240rZlw7a13uG6choBrsBbPg_83jbhxHb4zQ0jV3p2gw76xEU4_4jF6vv7zVN9ki_v5bX21yHROxJRpWUpK2xxoXxDGoNQcBOiWdrSXogWqqZBFIcocCsGF7EFrLXjeQl9qUXF-jH5vdVfrdgmdBjeFxqpVMMsmbJRvjPqacWZUg39VXAjJKpkEfn4IBP93DXFSL34dXJpZ0UqWXJZpiYn6taV08DE50e86UKLe7VE7exJ7tj_Sjvw0IwHnWyCmlBsg7LX8T-0N54iaiw</recordid><startdate>20120924</startdate><enddate>20120924</enddate><creator>Tsukahara, Tamotsu</creator><creator>Murakami-Murofushi, Kimiko</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20120924</creationdate><title>Release of Cyclic Phosphatidic Acid from Gelatin-based Hydrogels Inhibit Colon Cancer Cell Growth and Migration</title><author>Tsukahara, Tamotsu ; Murakami-Murofushi, Kimiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-c97911b5e1f6022e7c3e4ecb1d1f94be1c14966475e64349feccc435bef7c4833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>631/154/555</topic><topic>631/337/641/83</topic><topic>631/45/287</topic><topic>631/67/70</topic><topic>Bioavailability</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colorectal cancer</topic><topic>Controlled release</topic><topic>Drug delivery</topic><topic>Drug Delivery Systems</topic><topic>Gelatin</topic><topic>Gelatin - chemistry</topic><topic>Gelatin - pharmacology</topic><topic>HT29 Cells</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Hydrogels</topic><topic>Hydrogels - chemistry</topic><topic>Hydrogels - pharmacology</topic><topic>Lipids</topic><topic>Lysophospholipids</topic><topic>Microspheres</topic><topic>multidisciplinary</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Phosphatidic acid</topic><topic>Phosphatidic Acids - chemistry</topic><topic>Phosphatidic Acids - pharmacology</topic><topic>PPAR gamma - chemistry</topic><topic>PPAR gamma - genetics</topic><topic>Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsukahara, Tamotsu</creatorcontrib><creatorcontrib>Murakami-Murofushi, Kimiko</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsukahara, Tamotsu</au><au>Murakami-Murofushi, Kimiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Release of Cyclic Phosphatidic Acid from Gelatin-based Hydrogels Inhibit Colon Cancer Cell Growth and Migration</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2012-09-24</date><risdate>2012</risdate><volume>2</volume><issue>1</issue><spage>687</spage><pages>687-</pages><artnum>687</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Microparticle and nanoparticle formulations are widely used to improve the bioavailability of low-solubility drugs and as vehicles for organ- and tissue-specific targeted drug delivery. We investigated the effect of a novel, controlled-release form of a bioactive lipid, cyclic phosphatidic acid (cPA), on human colon cancer cell line functions. We encapsulated cPA in gelatin-based hydrogels and examined its ability to inhibit the viability and migration of HT-29 and DLD-1 cells
in vitro
and the LPA-induced activity of the transcription factor peroxisome proliferator-activated receptor gamma (PPARγ). The hydrogel delivery system prolonged cPA release into the culture medium. Accordingly, cPA-hydrogel microspheres substantially inhibited LPA-induced PPARγ activity and cell growth and migration compared with that of cells cultured with cPA alone. Thus, hydrogel microspheres are a potential system for stable and efficient delivery of bioactive lipids such as cPA and may offer a new strategy for targeted colon cancer treatment.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23008752</pmid><doi>10.1038/srep00687</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2012-09, Vol.2 (1), p.687, Article 687 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3449289 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature OA Free Journals; Nature Free; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 631/154/555 631/337/641/83 631/45/287 631/67/70 Bioavailability Cell culture Cell growth Cell Movement - drug effects Cell Proliferation - drug effects Colon cancer Colonic Neoplasms - drug therapy Colorectal cancer Controlled release Drug delivery Drug Delivery Systems Gelatin Gelatin - chemistry Gelatin - pharmacology HT29 Cells Humanities and Social Sciences Humans Hydrogels Hydrogels - chemistry Hydrogels - pharmacology Lipids Lysophospholipids Microspheres multidisciplinary Nanoparticles Nanoparticles - chemistry Phosphatidic acid Phosphatidic Acids - chemistry Phosphatidic Acids - pharmacology PPAR gamma - chemistry PPAR gamma - genetics Science |
| title | Release of Cyclic Phosphatidic Acid from Gelatin-based Hydrogels Inhibit Colon Cancer Cell Growth and Migration |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T21%3A55%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Release%20of%20Cyclic%20Phosphatidic%20Acid%20from%20Gelatin-based%20Hydrogels%20Inhibit%20Colon%20Cancer%20Cell%20Growth%20and%20Migration&rft.jtitle=Scientific%20reports&rft.au=Tsukahara,%20Tamotsu&rft.date=2012-09-24&rft.volume=2&rft.issue=1&rft.spage=687&rft.pages=687-&rft.artnum=687&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep00687&rft_dat=%3Cproquest_pubme%3E1897397230%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1897397230&rft_id=info:pmid/23008752&rfr_iscdi=true |