Constrained Bonding Environment in the Michaelis Complex of Trypanosoma cruzi Uridine Phosphorylase

The transition state for the Trypanosoma cruzi uridine phosphorylase (TcUP) reaction has an expanded SN2 character. We used binding isotope effects (BIE's) to probe uridine distortion in the complex with TcUP and sulfate to mimic the Michaelis complex. Inverse 1′-3H and 5′-3H BIE's indicat...

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