FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124
Mammalian brain-specific miR-9 and miR-124 have been implicated in several aspects of neuronal development and function. However, it is not known how their expression levels are regulated in vivo. We found that the levels of miR-9 and miR-124 are regulated by FXR1P but not by the loss of FXR2P or FM...
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| Veröffentlicht in: | The Journal of neuroscience 2011-09, Vol.31 (39), p.13705-13709 |
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| container_title | The Journal of neuroscience |
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| creator | Xu, Xia-Lian Zong, Ruiting Li, Zhaodong Biswas, Md Helal Uddin Fang, Zhe Nelson, David L Gao, Fen-Biao |
| description | Mammalian brain-specific miR-9 and miR-124 have been implicated in several aspects of neuronal development and function. However, it is not known how their expression levels are regulated in vivo. We found that the levels of miR-9 and miR-124 are regulated by FXR1P but not by the loss of FXR2P or FMRP in vivo, a mouse model of fragile X syndrome. Surprisingly, the levels of miR-9 and miR-124 are elevated in fmr1/fxr2 double-knock-out mice, in part reflecting posttranscriptional upregulation of FXR1P. Indeed, FXR1P is required for efficient processing of pre-miR-9 and pre-miR-124 in vitro and forms a complex with Dicer and pre-miRNAs. These findings reveal differential roles of FMRP family proteins in controlling the expression levels of brain-specific miRNAs. |
| doi_str_mv | 10.1523/JNEUROSCI.2827-11.2011 |
| format | Article |
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However, it is not known how their expression levels are regulated in vivo. We found that the levels of miR-9 and miR-124 are regulated by FXR1P but not by the loss of FXR2P or FMRP in vivo, a mouse model of fragile X syndrome. Surprisingly, the levels of miR-9 and miR-124 are elevated in fmr1/fxr2 double-knock-out mice, in part reflecting posttranscriptional upregulation of FXR1P. Indeed, FXR1P is required for efficient processing of pre-miR-9 and pre-miR-124 in vitro and forms a complex with Dicer and pre-miRNAs. 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However, it is not known how their expression levels are regulated in vivo. We found that the levels of miR-9 and miR-124 are regulated by FXR1P but not by the loss of FXR2P or FMRP in vivo, a mouse model of fragile X syndrome. Surprisingly, the levels of miR-9 and miR-124 are elevated in fmr1/fxr2 double-knock-out mice, in part reflecting posttranscriptional upregulation of FXR1P. Indeed, FXR1P is required for efficient processing of pre-miR-9 and pre-miR-124 in vitro and forms a complex with Dicer and pre-miRNAs. These findings reveal differential roles of FMRP family proteins in controlling the expression levels of brain-specific miRNAs.</description><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Brief Communications</subject><subject>Cells, Cultured</subject><subject>DEAD-box RNA Helicases - metabolism</subject><subject>Female</subject><subject>Fragile X Mental Retardation Protein - genetics</subject><subject>Fragile X Mental Retardation Protein - physiology</subject><subject>Fragile X Syndrome - genetics</subject><subject>Fragile X Syndrome - metabolism</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>MicroRNAs - biosynthesis</subject><subject>MicroRNAs - metabolism</subject><subject>Ribonuclease III - metabolism</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - physiology</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkVtv2zAMhYVhxZq1_QuB3vbkjJRky34ZUARNlyG9IG2BvgmyQqUafMksu8D-_Ry0zdongjiHhyQ-xqYIM0yF_P7r-uJhfXM3X85ELnSCOBOA-IlNRrVIhAL8zCYgNCSZ0uqYfY3xNwBoQP2FHQssUi2knDCzeFzjLS-HnjdtzxdX61ve0XaobE-R90_EK3qmKvLW89rWta2CbXjZ2dAkcUcu-OB4HVzXrq_Pk4LbZvO_RaFO2ZG3VaSz13rCHhYX9_Ofyermcjk_XyVOoewTkUGqSk8ASijpQCsofWG1T3XqlU8tIubZBgQ5rYhgU46v6bxwzssCfS5P2I-X3N1Q1rRx1PSdrcyuC7Xt_prWBvNRacKT2bbPRiqV6VyMAd9eA7r2z0CxN3WIjqrKNtQO0eRFmqdS52p0Zi_O8csYO_KHLQhmD8cc4Jg9HINo9nDGwen7Gw9jbzTkP0AVivc</recordid><startdate>20110928</startdate><enddate>20110928</enddate><creator>Xu, Xia-Lian</creator><creator>Zong, Ruiting</creator><creator>Li, Zhaodong</creator><creator>Biswas, Md Helal Uddin</creator><creator>Fang, Zhe</creator><creator>Nelson, David L</creator><creator>Gao, Fen-Biao</creator><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110928</creationdate><title>FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124</title><author>Xu, Xia-Lian ; Zong, Ruiting ; Li, Zhaodong ; Biswas, Md Helal Uddin ; Fang, Zhe ; Nelson, David L ; Gao, Fen-Biao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-26054bfe004243c0740bf9a7f575f4f5a11186d02ec74ee0db270789ccf391f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Brief Communications</topic><topic>Cells, Cultured</topic><topic>DEAD-box RNA Helicases - metabolism</topic><topic>Female</topic><topic>Fragile X Mental Retardation Protein - genetics</topic><topic>Fragile X Mental Retardation Protein - physiology</topic><topic>Fragile X Syndrome - genetics</topic><topic>Fragile X Syndrome - metabolism</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>MicroRNAs - biosynthesis</topic><topic>MicroRNAs - metabolism</topic><topic>Ribonuclease III - metabolism</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Xia-Lian</creatorcontrib><creatorcontrib>Zong, Ruiting</creatorcontrib><creatorcontrib>Li, Zhaodong</creatorcontrib><creatorcontrib>Biswas, Md Helal Uddin</creatorcontrib><creatorcontrib>Fang, Zhe</creatorcontrib><creatorcontrib>Nelson, David L</creatorcontrib><creatorcontrib>Gao, Fen-Biao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Xia-Lian</au><au>Zong, Ruiting</au><au>Li, Zhaodong</au><au>Biswas, Md Helal Uddin</au><au>Fang, Zhe</au><au>Nelson, David L</au><au>Gao, Fen-Biao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>2011-09-28</date><risdate>2011</risdate><volume>31</volume><issue>39</issue><spage>13705</spage><epage>13709</epage><pages>13705-13709</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>Mammalian brain-specific miR-9 and miR-124 have been implicated in several aspects of neuronal development and function. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Brain - metabolism Brief Communications Cells, Cultured DEAD-box RNA Helicases - metabolism Female Fragile X Mental Retardation Protein - genetics Fragile X Mental Retardation Protein - physiology Fragile X Syndrome - genetics Fragile X Syndrome - metabolism HEK293 Cells Humans Male Mice Mice, Knockout MicroRNAs - biosynthesis MicroRNAs - metabolism Ribonuclease III - metabolism RNA-Binding Proteins - genetics RNA-Binding Proteins - physiology |
| title | FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124 |
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