Attenuation of indomethacin-induced gastric mucosal injury by prophylactic administration of sake yeast-derived thioredoxin
Background Indomethacin is one of the group of nonsteroidal anti-inflammatory drugs, which often cause gastric mucosal injury as a side effect. Infiltration and activation of inflammatory cells, production of proinflammatory cytokines and chemokines, generation of reactive oxygen species, and activa...
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| Veröffentlicht in: | Journal of gastroenterology 2012-09, Vol.47 (9), p.978-987 |
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| creator | Nakajima, Atsushi Fukui, Toshiro Takahashi, Yu Kishimoto, Masanobu Yamashina, Masao Nakayama, Shinji Sakaguchi, Yutaku Yoshida, Katsunori Uchida, Kazushige Nishio, Akiyoshi Yodoi, Junji Okazaki, Kazuichi |
| description | Background
Indomethacin is one of the group of nonsteroidal anti-inflammatory drugs, which often cause gastric mucosal injury as a side effect. Infiltration and activation of inflammatory cells, production of proinflammatory cytokines and chemokines, generation of reactive oxygen species, and activation of apoptotic signaling are involved in the pathogenesis of indomethacin-induced gastric injury. We examined whether sake yeast-derived thioredoxin (a small redox-active protein with anti-oxidative activity and various redox-regulating functions) reduced indomethacin-induced gastric injury.
Methods
Gastric injury was produced by the intraperitoneal administration of indomethacin (40 mg/kg body weight) to C57BL/6 mice. Prior to the administration of indomethacin, the mice were offered food pellets containing non-genetically modified sake yeast-derived thioredoxin (thioredoxin 200 μg/g) for 3 days. Histological examinations, assessment of myeloperoxidase activity, and analysis of the gene expressions of proinflammatory cytokines and a chemokine (interleukin [IL]-1β, IL-6, and CXCL1) were statistically evaluated. Indomethacin cytotoxicity was determined by lactate dehydrogenase release from murine gastric epithelial GSM06 cells induced by 24-h treatment with 200 and 400 μM indomethacin after 1-h preincubation with 100 μg/ml sake yeast-derived thioredoxin.
Results
Macroscopic (edema, hemorrhage, and ulcers) and histological (necrosis, submucosal edema, neutrophil infiltration) findings induced by indomethacin were significantly reduced by pretreatment with food pellets containing thioredoxin. Gastric myeloperoxidase activity and the gene expressions of proinflammatory cytokines (IL-1β and IL-6) were also significantly reduced by this pretreatment compared with findings in the mice not pretreated with thioredoxin-containing food pellets. The administration of sake yeast-derived thioredoxin significantly reduced indomethacin-induced cytotoxicity in GSM06 cells.
Conclusions
We conclude that oral administration of sake yeast-derived thioredoxin reduces indomethacin-induced gastric injury. Sake yeast-derived thioredoxin may have therapeutic potential against indomethacin-induced gastric injury. |
| doi_str_mv | 10.1007/s00535-012-0564-5 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3443347</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A714585675</galeid><sourcerecordid>A714585675</sourcerecordid><originalsourceid>FETCH-LOGICAL-c689t-50d3bd232608ed870b21db4862d974146f3fbe0215b9b05e8fada34b6bfe16383</originalsourceid><addsrcrecordid>eNp1kkuLFDEUhYMoTjv6A9xIgRs3NeZZqdoIzeCoMOBG1yGPW91pq5I2qRps_POTosemR5QsQu79zkluOAi9JviKYCzfZ4wFEzUmtMai4bV4glaEl4roKH2KVrjjvCZE8gv0IucdxoRh0T5HF5RyTKXkK_R7PU0QZj35GKrYVz64OMK01daHuhxmC67a6Dwlb6txtjHroUC7OR0qc6j2Ke63h0HbqbS1G33wBT25Zf0DqgMUee0g-bviNW19TODiLx9eome9HjK8etgv0febj9-uP9e3Xz99uV7f1rZpu6kW2DHjKKMNbsG1EhtKnOFtQ10nOeFNz3oDmBJhOoMFtL12mnHTmB5Iw1p2iT4cffezGcFZCOWJg9onP-p0UFF79bgT_FZt4p1inDPGZTF492CQ4s8Z8qRGny0Mgw4Q56wIoYLShlNR0Ld_obs4p1DGUwSzTnSdbM-ojR5A-dDHcq9dTNVaEi5a0ciFuvoHVZaD0dsYoPel_khAjgKbYs4J-tOMBKslMeqYGFUSo5bEqEXz5vxzToo_ESkAPQK5tMIG0vlE_3O9B54dzcM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1039599785</pqid></control><display><type>article</type><title>Attenuation of indomethacin-induced gastric mucosal injury by prophylactic administration of sake yeast-derived thioredoxin</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Nakajima, Atsushi ; Fukui, Toshiro ; Takahashi, Yu ; Kishimoto, Masanobu ; Yamashina, Masao ; Nakayama, Shinji ; Sakaguchi, Yutaku ; Yoshida, Katsunori ; Uchida, Kazushige ; Nishio, Akiyoshi ; Yodoi, Junji ; Okazaki, Kazuichi</creator><creatorcontrib>Nakajima, Atsushi ; Fukui, Toshiro ; Takahashi, Yu ; Kishimoto, Masanobu ; Yamashina, Masao ; Nakayama, Shinji ; Sakaguchi, Yutaku ; Yoshida, Katsunori ; Uchida, Kazushige ; Nishio, Akiyoshi ; Yodoi, Junji ; Okazaki, Kazuichi</creatorcontrib><description>Background
Indomethacin is one of the group of nonsteroidal anti-inflammatory drugs, which often cause gastric mucosal injury as a side effect. Infiltration and activation of inflammatory cells, production of proinflammatory cytokines and chemokines, generation of reactive oxygen species, and activation of apoptotic signaling are involved in the pathogenesis of indomethacin-induced gastric injury. We examined whether sake yeast-derived thioredoxin (a small redox-active protein with anti-oxidative activity and various redox-regulating functions) reduced indomethacin-induced gastric injury.
Methods
Gastric injury was produced by the intraperitoneal administration of indomethacin (40 mg/kg body weight) to C57BL/6 mice. Prior to the administration of indomethacin, the mice were offered food pellets containing non-genetically modified sake yeast-derived thioredoxin (thioredoxin 200 μg/g) for 3 days. Histological examinations, assessment of myeloperoxidase activity, and analysis of the gene expressions of proinflammatory cytokines and a chemokine (interleukin [IL]-1β, IL-6, and CXCL1) were statistically evaluated. Indomethacin cytotoxicity was determined by lactate dehydrogenase release from murine gastric epithelial GSM06 cells induced by 24-h treatment with 200 and 400 μM indomethacin after 1-h preincubation with 100 μg/ml sake yeast-derived thioredoxin.
Results
Macroscopic (edema, hemorrhage, and ulcers) and histological (necrosis, submucosal edema, neutrophil infiltration) findings induced by indomethacin were significantly reduced by pretreatment with food pellets containing thioredoxin. Gastric myeloperoxidase activity and the gene expressions of proinflammatory cytokines (IL-1β and IL-6) were also significantly reduced by this pretreatment compared with findings in the mice not pretreated with thioredoxin-containing food pellets. The administration of sake yeast-derived thioredoxin significantly reduced indomethacin-induced cytotoxicity in GSM06 cells.
Conclusions
We conclude that oral administration of sake yeast-derived thioredoxin reduces indomethacin-induced gastric injury. Sake yeast-derived thioredoxin may have therapeutic potential against indomethacin-induced gastric injury.</description><identifier>ISSN: 0944-1174</identifier><identifier>EISSN: 1435-5922</identifier><identifier>DOI: 10.1007/s00535-012-0564-5</identifier><identifier>PMID: 22402774</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject><![CDATA[Abdominal Surgery ; Administration, Oral ; Analysis ; Animals ; Anti-Inflammatory Agents, Non-Steroidal - toxicity ; Apoptosis ; Chemokine CXCL1 - drug effects ; Chemokine CXCL1 - genetics ; Chemokine CXCL1 - metabolism ; Colorectal Surgery ; Cytokines - drug effects ; Cytokines - genetics ; Cytokines - metabolism ; Disease Models, Animal ; Female ; Fungal Proteins - administration & dosage ; Fungal Proteins - isolation & purification ; Gastric Mucosa - injuries ; Gastric Mucosa - pathology ; Gastroenterology ; Gene Expression ; Genetically modified organisms ; Hepatology ; Indexing in process ; Indomethacin ; Indomethacin - toxicity ; Interleukins ; Medicine ; Medicine & Public Health ; Mice ; Mice, Inbred C57BL ; Original Article—Alimentary Tract ; Original —Alimentary Tract ; Peroxidase - drug effects ; Peroxidase - metabolism ; Real-Time Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Saccharomyces cerevisiae - chemistry ; Stomach Diseases - chemically induced ; Stomach Diseases - pathology ; Stomach Diseases - prevention & control ; Surgical Oncology ; Thioredoxin ; Thioredoxins - administration & dosage ; Thioredoxins - isolation & purification]]></subject><ispartof>Journal of gastroenterology, 2012-09, Vol.47 (9), p.978-987</ispartof><rights>The Author(s) 2012</rights><rights>COPYRIGHT 2012 Springer</rights><rights>Springer 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c689t-50d3bd232608ed870b21db4862d974146f3fbe0215b9b05e8fada34b6bfe16383</citedby><cites>FETCH-LOGICAL-c689t-50d3bd232608ed870b21db4862d974146f3fbe0215b9b05e8fada34b6bfe16383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00535-012-0564-5$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00535-012-0564-5$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22402774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakajima, Atsushi</creatorcontrib><creatorcontrib>Fukui, Toshiro</creatorcontrib><creatorcontrib>Takahashi, Yu</creatorcontrib><creatorcontrib>Kishimoto, Masanobu</creatorcontrib><creatorcontrib>Yamashina, Masao</creatorcontrib><creatorcontrib>Nakayama, Shinji</creatorcontrib><creatorcontrib>Sakaguchi, Yutaku</creatorcontrib><creatorcontrib>Yoshida, Katsunori</creatorcontrib><creatorcontrib>Uchida, Kazushige</creatorcontrib><creatorcontrib>Nishio, Akiyoshi</creatorcontrib><creatorcontrib>Yodoi, Junji</creatorcontrib><creatorcontrib>Okazaki, Kazuichi</creatorcontrib><title>Attenuation of indomethacin-induced gastric mucosal injury by prophylactic administration of sake yeast-derived thioredoxin</title><title>Journal of gastroenterology</title><addtitle>J Gastroenterol</addtitle><addtitle>J Gastroenterol</addtitle><description>Background
Indomethacin is one of the group of nonsteroidal anti-inflammatory drugs, which often cause gastric mucosal injury as a side effect. Infiltration and activation of inflammatory cells, production of proinflammatory cytokines and chemokines, generation of reactive oxygen species, and activation of apoptotic signaling are involved in the pathogenesis of indomethacin-induced gastric injury. We examined whether sake yeast-derived thioredoxin (a small redox-active protein with anti-oxidative activity and various redox-regulating functions) reduced indomethacin-induced gastric injury.
Methods
Gastric injury was produced by the intraperitoneal administration of indomethacin (40 mg/kg body weight) to C57BL/6 mice. Prior to the administration of indomethacin, the mice were offered food pellets containing non-genetically modified sake yeast-derived thioredoxin (thioredoxin 200 μg/g) for 3 days. Histological examinations, assessment of myeloperoxidase activity, and analysis of the gene expressions of proinflammatory cytokines and a chemokine (interleukin [IL]-1β, IL-6, and CXCL1) were statistically evaluated. Indomethacin cytotoxicity was determined by lactate dehydrogenase release from murine gastric epithelial GSM06 cells induced by 24-h treatment with 200 and 400 μM indomethacin after 1-h preincubation with 100 μg/ml sake yeast-derived thioredoxin.
Results
Macroscopic (edema, hemorrhage, and ulcers) and histological (necrosis, submucosal edema, neutrophil infiltration) findings induced by indomethacin were significantly reduced by pretreatment with food pellets containing thioredoxin. Gastric myeloperoxidase activity and the gene expressions of proinflammatory cytokines (IL-1β and IL-6) were also significantly reduced by this pretreatment compared with findings in the mice not pretreated with thioredoxin-containing food pellets. The administration of sake yeast-derived thioredoxin significantly reduced indomethacin-induced cytotoxicity in GSM06 cells.
Conclusions
We conclude that oral administration of sake yeast-derived thioredoxin reduces indomethacin-induced gastric injury. Sake yeast-derived thioredoxin may have therapeutic potential against indomethacin-induced gastric injury.</description><subject>Abdominal Surgery</subject><subject>Administration, Oral</subject><subject>Analysis</subject><subject>Animals</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - toxicity</subject><subject>Apoptosis</subject><subject>Chemokine CXCL1 - drug effects</subject><subject>Chemokine CXCL1 - genetics</subject><subject>Chemokine CXCL1 - metabolism</subject><subject>Colorectal Surgery</subject><subject>Cytokines - drug effects</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Fungal Proteins - administration & dosage</subject><subject>Fungal Proteins - isolation & purification</subject><subject>Gastric Mucosa - injuries</subject><subject>Gastric Mucosa - pathology</subject><subject>Gastroenterology</subject><subject>Gene Expression</subject><subject>Genetically modified organisms</subject><subject>Hepatology</subject><subject>Indexing in process</subject><subject>Indomethacin</subject><subject>Indomethacin - toxicity</subject><subject>Interleukins</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Original Article—Alimentary Tract</subject><subject>Original —Alimentary Tract</subject><subject>Peroxidase - drug effects</subject><subject>Peroxidase - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Saccharomyces cerevisiae - chemistry</subject><subject>Stomach Diseases - chemically induced</subject><subject>Stomach Diseases - pathology</subject><subject>Stomach Diseases - prevention & control</subject><subject>Surgical Oncology</subject><subject>Thioredoxin</subject><subject>Thioredoxins - administration & dosage</subject><subject>Thioredoxins - isolation & purification</subject><issn>0944-1174</issn><issn>1435-5922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kkuLFDEUhYMoTjv6A9xIgRs3NeZZqdoIzeCoMOBG1yGPW91pq5I2qRps_POTosemR5QsQu79zkluOAi9JviKYCzfZ4wFEzUmtMai4bV4glaEl4roKH2KVrjjvCZE8gv0IucdxoRh0T5HF5RyTKXkK_R7PU0QZj35GKrYVz64OMK01daHuhxmC67a6Dwlb6txtjHroUC7OR0qc6j2Ke63h0HbqbS1G33wBT25Zf0DqgMUee0g-bviNW19TODiLx9eome9HjK8etgv0febj9-uP9e3Xz99uV7f1rZpu6kW2DHjKKMNbsG1EhtKnOFtQ10nOeFNz3oDmBJhOoMFtL12mnHTmB5Iw1p2iT4cffezGcFZCOWJg9onP-p0UFF79bgT_FZt4p1inDPGZTF492CQ4s8Z8qRGny0Mgw4Q56wIoYLShlNR0Ld_obs4p1DGUwSzTnSdbM-ojR5A-dDHcq9dTNVaEi5a0ciFuvoHVZaD0dsYoPel_khAjgKbYs4J-tOMBKslMeqYGFUSo5bEqEXz5vxzToo_ESkAPQK5tMIG0vlE_3O9B54dzcM</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Nakajima, Atsushi</creator><creator>Fukui, Toshiro</creator><creator>Takahashi, Yu</creator><creator>Kishimoto, Masanobu</creator><creator>Yamashina, Masao</creator><creator>Nakayama, Shinji</creator><creator>Sakaguchi, Yutaku</creator><creator>Yoshida, Katsunori</creator><creator>Uchida, Kazushige</creator><creator>Nishio, Akiyoshi</creator><creator>Yodoi, Junji</creator><creator>Okazaki, Kazuichi</creator><general>Springer Japan</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20120901</creationdate><title>Attenuation of indomethacin-induced gastric mucosal injury by prophylactic administration of sake yeast-derived thioredoxin</title><author>Nakajima, Atsushi ; Fukui, Toshiro ; Takahashi, Yu ; Kishimoto, Masanobu ; Yamashina, Masao ; Nakayama, Shinji ; Sakaguchi, Yutaku ; Yoshida, Katsunori ; Uchida, Kazushige ; Nishio, Akiyoshi ; Yodoi, Junji ; Okazaki, Kazuichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c689t-50d3bd232608ed870b21db4862d974146f3fbe0215b9b05e8fada34b6bfe16383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Abdominal Surgery</topic><topic>Administration, Oral</topic><topic>Analysis</topic><topic>Animals</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - toxicity</topic><topic>Apoptosis</topic><topic>Chemokine CXCL1 - drug effects</topic><topic>Chemokine CXCL1 - genetics</topic><topic>Chemokine CXCL1 - metabolism</topic><topic>Colorectal Surgery</topic><topic>Cytokines - drug effects</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Fungal Proteins - administration & dosage</topic><topic>Fungal Proteins - isolation & purification</topic><topic>Gastric Mucosa - injuries</topic><topic>Gastric Mucosa - pathology</topic><topic>Gastroenterology</topic><topic>Gene Expression</topic><topic>Genetically modified organisms</topic><topic>Hepatology</topic><topic>Indexing in process</topic><topic>Indomethacin</topic><topic>Indomethacin - toxicity</topic><topic>Interleukins</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Original Article—Alimentary Tract</topic><topic>Original —Alimentary Tract</topic><topic>Peroxidase - drug effects</topic><topic>Peroxidase - metabolism</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Saccharomyces cerevisiae - chemistry</topic><topic>Stomach Diseases - chemically induced</topic><topic>Stomach Diseases - pathology</topic><topic>Stomach Diseases - prevention & control</topic><topic>Surgical Oncology</topic><topic>Thioredoxin</topic><topic>Thioredoxins - administration & dosage</topic><topic>Thioredoxins - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakajima, Atsushi</creatorcontrib><creatorcontrib>Fukui, Toshiro</creatorcontrib><creatorcontrib>Takahashi, Yu</creatorcontrib><creatorcontrib>Kishimoto, Masanobu</creatorcontrib><creatorcontrib>Yamashina, Masao</creatorcontrib><creatorcontrib>Nakayama, Shinji</creatorcontrib><creatorcontrib>Sakaguchi, Yutaku</creatorcontrib><creatorcontrib>Yoshida, Katsunori</creatorcontrib><creatorcontrib>Uchida, Kazushige</creatorcontrib><creatorcontrib>Nishio, Akiyoshi</creatorcontrib><creatorcontrib>Yodoi, Junji</creatorcontrib><creatorcontrib>Okazaki, Kazuichi</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of gastroenterology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakajima, Atsushi</au><au>Fukui, Toshiro</au><au>Takahashi, Yu</au><au>Kishimoto, Masanobu</au><au>Yamashina, Masao</au><au>Nakayama, Shinji</au><au>Sakaguchi, Yutaku</au><au>Yoshida, Katsunori</au><au>Uchida, Kazushige</au><au>Nishio, Akiyoshi</au><au>Yodoi, Junji</au><au>Okazaki, Kazuichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Attenuation of indomethacin-induced gastric mucosal injury by prophylactic administration of sake yeast-derived thioredoxin</atitle><jtitle>Journal of gastroenterology</jtitle><stitle>J Gastroenterol</stitle><addtitle>J Gastroenterol</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>47</volume><issue>9</issue><spage>978</spage><epage>987</epage><pages>978-987</pages><issn>0944-1174</issn><eissn>1435-5922</eissn><abstract>Background
Indomethacin is one of the group of nonsteroidal anti-inflammatory drugs, which often cause gastric mucosal injury as a side effect. Infiltration and activation of inflammatory cells, production of proinflammatory cytokines and chemokines, generation of reactive oxygen species, and activation of apoptotic signaling are involved in the pathogenesis of indomethacin-induced gastric injury. We examined whether sake yeast-derived thioredoxin (a small redox-active protein with anti-oxidative activity and various redox-regulating functions) reduced indomethacin-induced gastric injury.
Methods
Gastric injury was produced by the intraperitoneal administration of indomethacin (40 mg/kg body weight) to C57BL/6 mice. Prior to the administration of indomethacin, the mice were offered food pellets containing non-genetically modified sake yeast-derived thioredoxin (thioredoxin 200 μg/g) for 3 days. Histological examinations, assessment of myeloperoxidase activity, and analysis of the gene expressions of proinflammatory cytokines and a chemokine (interleukin [IL]-1β, IL-6, and CXCL1) were statistically evaluated. Indomethacin cytotoxicity was determined by lactate dehydrogenase release from murine gastric epithelial GSM06 cells induced by 24-h treatment with 200 and 400 μM indomethacin after 1-h preincubation with 100 μg/ml sake yeast-derived thioredoxin.
Results
Macroscopic (edema, hemorrhage, and ulcers) and histological (necrosis, submucosal edema, neutrophil infiltration) findings induced by indomethacin were significantly reduced by pretreatment with food pellets containing thioredoxin. Gastric myeloperoxidase activity and the gene expressions of proinflammatory cytokines (IL-1β and IL-6) were also significantly reduced by this pretreatment compared with findings in the mice not pretreated with thioredoxin-containing food pellets. The administration of sake yeast-derived thioredoxin significantly reduced indomethacin-induced cytotoxicity in GSM06 cells.
Conclusions
We conclude that oral administration of sake yeast-derived thioredoxin reduces indomethacin-induced gastric injury. Sake yeast-derived thioredoxin may have therapeutic potential against indomethacin-induced gastric injury.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>22402774</pmid><doi>10.1007/s00535-012-0564-5</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of gastroenterology, 2012-09, Vol.47 (9), p.978-987 |
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| subjects | Abdominal Surgery Administration, Oral Analysis Animals Anti-Inflammatory Agents, Non-Steroidal - toxicity Apoptosis Chemokine CXCL1 - drug effects Chemokine CXCL1 - genetics Chemokine CXCL1 - metabolism Colorectal Surgery Cytokines - drug effects Cytokines - genetics Cytokines - metabolism Disease Models, Animal Female Fungal Proteins - administration & dosage Fungal Proteins - isolation & purification Gastric Mucosa - injuries Gastric Mucosa - pathology Gastroenterology Gene Expression Genetically modified organisms Hepatology Indexing in process Indomethacin Indomethacin - toxicity Interleukins Medicine Medicine & Public Health Mice Mice, Inbred C57BL Original Article—Alimentary Tract Original —Alimentary Tract Peroxidase - drug effects Peroxidase - metabolism Real-Time Polymerase Chain Reaction RNA, Messenger - metabolism Saccharomyces cerevisiae - chemistry Stomach Diseases - chemically induced Stomach Diseases - pathology Stomach Diseases - prevention & control Surgical Oncology Thioredoxin Thioredoxins - administration & dosage Thioredoxins - isolation & purification |
| title | Attenuation of indomethacin-induced gastric mucosal injury by prophylactic administration of sake yeast-derived thioredoxin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T12%3A22%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Attenuation%20of%20indomethacin-induced%20gastric%20mucosal%20injury%20by%20prophylactic%20administration%20of%20sake%20yeast-derived%20thioredoxin&rft.jtitle=Journal%20of%20gastroenterology&rft.au=Nakajima,%20Atsushi&rft.date=2012-09-01&rft.volume=47&rft.issue=9&rft.spage=978&rft.epage=987&rft.pages=978-987&rft.issn=0944-1174&rft.eissn=1435-5922&rft_id=info:doi/10.1007/s00535-012-0564-5&rft_dat=%3Cgale_pubme%3EA714585675%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1039599785&rft_id=info:pmid/22402774&rft_galeid=A714585675&rfr_iscdi=true |