Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma
To advance our knowledge on the snake venom composition and transcripts expressed in venom gland at the molecular level, we constructed a cDNA library from the venom gland of Agkistrodon piscivorus leucostoma for the generation of expressed sequence tags (ESTs) database. From the randomly sequenced...
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| Veröffentlicht in: | Toxicon (Oxford) 2008-06, Vol.51 (8), p.1457-1466 |
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| creator | Jia, Ying Cantu, Bruno A. Sánchez, Elda E. Pérez, John C. |
| description | To advance our knowledge on the snake venom composition and transcripts expressed in venom gland at the molecular level, we constructed a cDNA library from the venom gland of
Agkistrodon piscivorus leucostoma for the generation of expressed sequence tags (ESTs) database. From the randomly sequenced 2112 independent clones, we have obtained ESTs for 1309 (62%) cDNAs, which showed significant deduced amino acid sequence similarity (scores >80) to previously characterized proteins in National Center for Biotechnology Information (NCBI) database. Ribosomal proteins make up 47 clones (2%) and the remaining 756 (36%) cDNAs represent either unknown identity or show BLASTX sequence identity scores of |
| doi_str_mv | 10.1016/j.toxicon.2008.03.028 |
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Agkistrodon piscivorus leucostoma for the generation of expressed sequence tags (ESTs) database. From the randomly sequenced 2112 independent clones, we have obtained ESTs for 1309 (62%) cDNAs, which showed significant deduced amino acid sequence similarity (scores >80) to previously characterized proteins in National Center for Biotechnology Information (NCBI) database. Ribosomal proteins make up 47 clones (2%) and the remaining 756 (36%) cDNAs represent either unknown identity or show BLASTX sequence identity scores of <80 with known GenBank accessions. The most highly expressed gene encoding phospholipase A
2 (PLA
2) accounting for 35% of
A. p. leucostoma venom gland cDNAs was identified and further confirmed by crude venom applied to sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis and protein sequencing. A total of 180 representative genes were obtained from the sequence assemblies and deposited to EST database. Clones showing sequence identity to
disintegrins,
thrombin-like enzymes,
hemorrhagic toxins, fibrinogen clotting inhibitors and
plasminogen activators were also identified in our EST database. These data can be used to develop a research program that will help us identify genes encoding proteins that are of medical importance or proteins involved in the mechanisms of the toxin venom.</description><identifier>ISSN: 0041-0101</identifier><identifier>EISSN: 1879-3150</identifier><identifier>DOI: 10.1016/j.toxicon.2008.03.028</identifier><identifier>PMID: 18502463</identifier><identifier>CODEN: TOXIA6</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Agkistrodon - genetics ; Agkistrodon - metabolism ; Agkistrodon piscivorus leucostoma ; Amino Acid Sequence ; Animal poisons toxicology. Antivenoms ; Animals ; Base Sequence ; Biological and medical sciences ; cDNA library ; Cloning, Molecular ; Crotalid Venoms - chemistry ; DNA, Complementary - chemistry ; Electrophoresis, Polyacrylamide Gel ; ESTs ; Expressed Sequence Tags ; Gene Library ; Medical sciences ; Molecular Sequence Data ; Phospholipase A 2 ; Phospholipases A - analysis ; Phospholipases A - genetics ; RNA, Messenger - chemistry ; Sequence Alignment ; Sequence Analysis, DNA ; Toxicology ; Venoms</subject><ispartof>Toxicon (Oxford), 2008-06, Vol.51 (8), p.1457-1466</ispartof><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><rights>2008 Elsevier Ltd. All rights reserved. 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-30c0a5c8fc9558a90a35482420fef87bb455dc7f37f1e3915050092c1b20e6b73</citedby><cites>FETCH-LOGICAL-c526t-30c0a5c8fc9558a90a35482420fef87bb455dc7f37f1e3915050092c1b20e6b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.toxicon.2008.03.028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20470536$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18502463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jia, Ying</creatorcontrib><creatorcontrib>Cantu, Bruno A.</creatorcontrib><creatorcontrib>Sánchez, Elda E.</creatorcontrib><creatorcontrib>Pérez, John C.</creatorcontrib><title>Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma</title><title>Toxicon (Oxford)</title><addtitle>Toxicon</addtitle><description>To advance our knowledge on the snake venom composition and transcripts expressed in venom gland at the molecular level, we constructed a cDNA library from the venom gland of
Agkistrodon piscivorus leucostoma for the generation of expressed sequence tags (ESTs) database. From the randomly sequenced 2112 independent clones, we have obtained ESTs for 1309 (62%) cDNAs, which showed significant deduced amino acid sequence similarity (scores >80) to previously characterized proteins in National Center for Biotechnology Information (NCBI) database. Ribosomal proteins make up 47 clones (2%) and the remaining 756 (36%) cDNAs represent either unknown identity or show BLASTX sequence identity scores of <80 with known GenBank accessions. The most highly expressed gene encoding phospholipase A
2 (PLA
2) accounting for 35% of
A. p. leucostoma venom gland cDNAs was identified and further confirmed by crude venom applied to sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis and protein sequencing. A total of 180 representative genes were obtained from the sequence assemblies and deposited to EST database. Clones showing sequence identity to
disintegrins,
thrombin-like enzymes,
hemorrhagic toxins, fibrinogen clotting inhibitors and
plasminogen activators were also identified in our EST database. These data can be used to develop a research program that will help us identify genes encoding proteins that are of medical importance or proteins involved in the mechanisms of the toxin venom.</description><subject>Agkistrodon - genetics</subject><subject>Agkistrodon - metabolism</subject><subject>Agkistrodon piscivorus leucostoma</subject><subject>Amino Acid Sequence</subject><subject>Animal poisons toxicology. Antivenoms</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cDNA library</subject><subject>Cloning, Molecular</subject><subject>Crotalid Venoms - chemistry</subject><subject>DNA, Complementary - chemistry</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>ESTs</subject><subject>Expressed Sequence Tags</subject><subject>Gene Library</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Phospholipase A 2</subject><subject>Phospholipases A - analysis</subject><subject>Phospholipases A - genetics</subject><subject>RNA, Messenger - chemistry</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Toxicology</subject><subject>Venoms</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO0zAQhi0EYrsLjwDyBW4JYztOnAuoKiwgrRYJwdlyHbvrksTFTip4e6ZqtMBpT3OYb0bzz0fICwYlA1a_2ZdT_BVsHEsOoEoQJXD1iKyYatpCMAmPyQqgYgUgfkEuc94DgFBt_ZRcMCWBV7VYkXkTh0PvBjdOJv2m72_XNLufsxttGHfUjB0NHfaCD9ZMIY40ejp8vV1n6lMc6HTn6NGNcYhzprv-xCOw3v0IeUqxQ_4Qsg3HmLDfu9nGPMXBPCNPvOmze77UK_L9-sO3zafi5svHz5v1TWElr6dCgAUjrfK2lVKZFoyQleIVB--8arbbSsrONl40njnRYmgJ0HLLthxcvW3EFXl73nuYt4PrLCZJpteHFAZMq6MJ-v_OGO70Lh61qETTcoELXi8LUsSv5EkPmMf1mNRhZN2wWla1Ug-CrK1bkEwiKM-gTTHn5Pz9NQz0yaze68WsPpnVIDSaxbmX_0b5O7WoRODVAphsTe-TQYf5nuNQNSBFjdy7M-fw8cfgkkZB6Nt1ITk76S6GB075A1Icx1s</recordid><startdate>20080615</startdate><enddate>20080615</enddate><creator>Jia, Ying</creator><creator>Cantu, Bruno A.</creator><creator>Sánchez, Elda E.</creator><creator>Pérez, John C.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080615</creationdate><title>Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma</title><author>Jia, Ying ; Cantu, Bruno A. ; Sánchez, Elda E. ; Pérez, John C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-30c0a5c8fc9558a90a35482420fef87bb455dc7f37f1e3915050092c1b20e6b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agkistrodon - genetics</topic><topic>Agkistrodon - metabolism</topic><topic>Agkistrodon piscivorus leucostoma</topic><topic>Amino Acid Sequence</topic><topic>Animal poisons toxicology. Antivenoms</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cDNA library</topic><topic>Cloning, Molecular</topic><topic>Crotalid Venoms - chemistry</topic><topic>DNA, Complementary - chemistry</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>ESTs</topic><topic>Expressed Sequence Tags</topic><topic>Gene Library</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Phospholipase A 2</topic><topic>Phospholipases A - analysis</topic><topic>Phospholipases A - genetics</topic><topic>RNA, Messenger - chemistry</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Toxicology</topic><topic>Venoms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jia, Ying</creatorcontrib><creatorcontrib>Cantu, Bruno A.</creatorcontrib><creatorcontrib>Sánchez, Elda E.</creatorcontrib><creatorcontrib>Pérez, John C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jia, Ying</au><au>Cantu, Bruno A.</au><au>Sánchez, Elda E.</au><au>Pérez, John C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>2008-06-15</date><risdate>2008</risdate><volume>51</volume><issue>8</issue><spage>1457</spage><epage>1466</epage><pages>1457-1466</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><coden>TOXIA6</coden><abstract>To advance our knowledge on the snake venom composition and transcripts expressed in venom gland at the molecular level, we constructed a cDNA library from the venom gland of
Agkistrodon piscivorus leucostoma for the generation of expressed sequence tags (ESTs) database. From the randomly sequenced 2112 independent clones, we have obtained ESTs for 1309 (62%) cDNAs, which showed significant deduced amino acid sequence similarity (scores >80) to previously characterized proteins in National Center for Biotechnology Information (NCBI) database. Ribosomal proteins make up 47 clones (2%) and the remaining 756 (36%) cDNAs represent either unknown identity or show BLASTX sequence identity scores of <80 with known GenBank accessions. The most highly expressed gene encoding phospholipase A
2 (PLA
2) accounting for 35% of
A. p. leucostoma venom gland cDNAs was identified and further confirmed by crude venom applied to sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis and protein sequencing. A total of 180 representative genes were obtained from the sequence assemblies and deposited to EST database. Clones showing sequence identity to
disintegrins,
thrombin-like enzymes,
hemorrhagic toxins, fibrinogen clotting inhibitors and
plasminogen activators were also identified in our EST database. These data can be used to develop a research program that will help us identify genes encoding proteins that are of medical importance or proteins involved in the mechanisms of the toxin venom.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18502463</pmid><doi>10.1016/j.toxicon.2008.03.028</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Toxicon (Oxford), 2008-06, Vol.51 (8), p.1457-1466 |
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| subjects | Agkistrodon - genetics Agkistrodon - metabolism Agkistrodon piscivorus leucostoma Amino Acid Sequence Animal poisons toxicology. Antivenoms Animals Base Sequence Biological and medical sciences cDNA library Cloning, Molecular Crotalid Venoms - chemistry DNA, Complementary - chemistry Electrophoresis, Polyacrylamide Gel ESTs Expressed Sequence Tags Gene Library Medical sciences Molecular Sequence Data Phospholipase A 2 Phospholipases A - analysis Phospholipases A - genetics RNA, Messenger - chemistry Sequence Alignment Sequence Analysis, DNA Toxicology Venoms |
| title | Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma |
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