Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities

The culture medium of Pseudomonas BAL 31 contains endonuclease activities which are highly specific for single-stranded DNA and for the single-stranded or weakly hydrogen-bonded regions in supercoiled closed circular DNA. Exposure of nicked DNA to the culture medium results in cleavage of the strand...

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Veröffentlicht in:	Nucleic acids research 1975-09, Vol.2 (9), p.1459-1492
Hauptverfasser:	Gray, Horace B., Ostrander, Daryl A., Hodnett, James L., Legerski, Randy J., Robberson, Donald L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Calcium - pharmacology Centrifugation, Density Gradient Deoxyribonucleases - isolation & purification Deoxyribonucleases - metabolism DNA, Single-Stranded DNA, Viral Endonucleases - isolation & purification Endonucleases - metabolism Exonucleases - metabolism Magnesium - pharmacology Microscopy, Electron Osmolar Concentration Pseudomonas Pseudomonas - enzymology Sodium Dodecyl Sulfate - pharmacology
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