Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria

Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria

Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal. supernatant of rat liver mitochondria. Both mRRA's contain rather short poly(A) tracts (40–70 mononucleotides) according to a profile of their elution...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nucleic acids research 1977-12, Vol.4 (12), p.4411-4424
Hauptverfasser: Kisselev, O.I., Gaitskhoki, V.S., Neifakh, S.A.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Kinetics
Mitochondria, Liver - analysis
Molecular Weight
Poly A - analysis
Polyribosomes - analysis
Protein Biosynthesis
Rats
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
Templates, Genetic
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 4424
container_issue 12
container_start_page 4411
container_title Nucleic acids research
container_volume 4
creator Kisselev, O.I.
Gaitskhoki, V.S.
Neifakh, S.A.
description Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal. supernatant of rat liver mitochondria. Both mRRA's contain rather short poly(A) tracts (40–70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated. mitochondrial lysate programmed. the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltona which was soluble in the neutral chloroform-methanol mixture.
doi_str_mv 10.1093/nar/4.12.4411
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_343249</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73808714</sourcerecordid><originalsourceid>FETCH-LOGICAL-c449t-f5d7326159b7297cb505aa27ebe614bdc97681fa693dbff0d9982bf86c5ba6f83</originalsourceid><addsrcrecordid>eNqFkUtvEzEUhS3EKxSW7Fh4hcpiUnv8Gi9YhKo0lSoQL6liY93x2Ilhxg62U1F-PalSBVixutI93zm6Vweh55TMKdHsJEI-4XPazjmn9B6aUSbbhmvZ3kczwohoKOHdY_SklG-EUE4Ff4QeSkI6QmdovChphBpSxBAHvIFcA4zYriGDrS6HX3sxebxJ4w0-XrxqbIoVQgxxhdVcfMKTK8XFlcv447sF9jlNOEPFY7jeraZQk12nOOQAT9EDD2Nxz-7mEfry9uzz6bK5fH9-cbq4bCznujZeDIq1kgrdq1Yr2wsiAFrleicp7werleyoB6nZ0HtPBq27tvedtKIH6Tt2hF7vczfbfnKDdbFmGM0mhwnyjUkQzL9KDGuzSteGcdZyvfO_vPPn9GPrSjVTKNaNI0SXtsUo1pFOUf5fkGrGO0bIDmz2oM2plOz84RhKzG2LZtei4Ya25rbFHf_i7w8O9L62P3GhVPfzoEL-bqRiSpjl1VfDP7xZLq-ENOfsN22yqXI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19348300</pqid></control><display><type>article</type><title>Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><source>PubMed Central</source><creator>Kisselev, O.I. ; Gaitskhoki, V.S. ; Neifakh, S.A.</creator><creatorcontrib>Kisselev, O.I. ; Gaitskhoki, V.S. ; Neifakh, S.A.</creatorcontrib><description>Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal. supernatant of rat liver mitochondria. Both mRRA's contain rather short poly(A) tracts (40–70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated. mitochondrial lysate programmed. the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltona which was soluble in the neutral chloroform-methanol mixture.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/4.12.4411</identifier><identifier>PMID: 600801</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Kinetics ; Mitochondria, Liver - analysis ; Molecular Weight ; Poly A - analysis ; Polyribosomes - analysis ; Protein Biosynthesis ; Rats ; RNA, Messenger - isolation &amp; purification ; RNA, Messenger - metabolism ; Templates, Genetic</subject><ispartof>Nucleic acids research, 1977-12, Vol.4 (12), p.4411-4424</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-f5d7326159b7297cb505aa27ebe614bdc97681fa693dbff0d9982bf86c5ba6f83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC343249/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC343249/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/600801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kisselev, O.I.</creatorcontrib><creatorcontrib>Gaitskhoki, V.S.</creatorcontrib><creatorcontrib>Neifakh, S.A.</creatorcontrib><title>Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal. supernatant of rat liver mitochondria. Both mRRA's contain rather short poly(A) tracts (40–70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated. mitochondrial lysate programmed. the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltona which was soluble in the neutral chloroform-methanol mixture.</description><subject>Animals</subject><subject>Kinetics</subject><subject>Mitochondria, Liver - analysis</subject><subject>Molecular Weight</subject><subject>Poly A - analysis</subject><subject>Polyribosomes - analysis</subject><subject>Protein Biosynthesis</subject><subject>Rats</subject><subject>RNA, Messenger - isolation &amp; purification</subject><subject>RNA, Messenger - metabolism</subject><subject>Templates, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhS3EKxSW7Fh4hcpiUnv8Gi9YhKo0lSoQL6liY93x2Ilhxg62U1F-PalSBVixutI93zm6Vweh55TMKdHsJEI-4XPazjmn9B6aUSbbhmvZ3kczwohoKOHdY_SklG-EUE4Ff4QeSkI6QmdovChphBpSxBAHvIFcA4zYriGDrS6HX3sxebxJ4w0-XrxqbIoVQgxxhdVcfMKTK8XFlcv447sF9jlNOEPFY7jeraZQk12nOOQAT9EDD2Nxz-7mEfry9uzz6bK5fH9-cbq4bCznujZeDIq1kgrdq1Yr2wsiAFrleicp7werleyoB6nZ0HtPBq27tvedtKIH6Tt2hF7vczfbfnKDdbFmGM0mhwnyjUkQzL9KDGuzSteGcdZyvfO_vPPn9GPrSjVTKNaNI0SXtsUo1pFOUf5fkGrGO0bIDmz2oM2plOz84RhKzG2LZtei4Ya25rbFHf_i7w8O9L62P3GhVPfzoEL-bqRiSpjl1VfDP7xZLq-ENOfsN22yqXI</recordid><startdate>197712</startdate><enddate>197712</enddate><creator>Kisselev, O.I.</creator><creator>Gaitskhoki, V.S.</creator><creator>Neifakh, S.A.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>197712</creationdate><title>Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria</title><author>Kisselev, O.I. ; Gaitskhoki, V.S. ; Neifakh, S.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-f5d7326159b7297cb505aa27ebe614bdc97681fa693dbff0d9982bf86c5ba6f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Animals</topic><topic>Kinetics</topic><topic>Mitochondria, Liver - analysis</topic><topic>Molecular Weight</topic><topic>Poly A - analysis</topic><topic>Polyribosomes - analysis</topic><topic>Protein Biosynthesis</topic><topic>Rats</topic><topic>RNA, Messenger - isolation &amp; purification</topic><topic>RNA, Messenger - metabolism</topic><topic>Templates, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kisselev, O.I.</creatorcontrib><creatorcontrib>Gaitskhoki, V.S.</creatorcontrib><creatorcontrib>Neifakh, S.A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kisselev, O.I.</au><au>Gaitskhoki, V.S.</au><au>Neifakh, S.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1977-12</date><risdate>1977</risdate><volume>4</volume><issue>12</issue><spage>4411</spage><epage>4424</epage><pages>4411-4424</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal. supernatant of rat liver mitochondria. Both mRRA's contain rather short poly(A) tracts (40–70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated. mitochondrial lysate programmed. the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltona which was soluble in the neutral chloroform-methanol mixture.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>600801</pmid><doi>10.1093/nar/4.12.4411</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0305-1048
ispartof Nucleic acids research, 1977-12, Vol.4 (12), p.4411-4424
issn 0305-1048
1362-4962
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_343249
source MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central
subjects Animals
Kinetics
Mitochondria, Liver - analysis
Molecular Weight
Poly A - analysis
Polyribosomes - analysis
Protein Biosynthesis
Rats
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
Templates, Genetic
title Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T13%3A18%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20partial%20characterization%20of%20poly%20(A)-containing%207.5S%20messenger%20RNA%20from%20rat%20liver%20mitochondria&rft.jtitle=Nucleic%20acids%20research&rft.au=Kisselev,%20O.I.&rft.date=1977-12&rft.volume=4&rft.issue=12&rft.spage=4411&rft.epage=4424&rft.pages=4411-4424&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/4.12.4411&rft_dat=%3Cproquest_pubme%3E73808714%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19348300&rft_id=info:pmid/600801&rfr_iscdi=true