Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals
Abstract Even though intracytoplasmic sperm injection (ICSI) has been widely used for the production of offspring in human infertility clinics and in reproductive research laboratories using mice, many researchers engaged in animal transgenesis still consider it somewhat cumbersome. The greatest adv...
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| Veröffentlicht in: | Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2009-03, Vol.32 (2), p.47-60 |
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| container_title | Comparative immunology, microbiology and infectious diseases |
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| creator | Moisyadi, Stefan Kaminski, Joseph M Yanagimachi, Ryuzo |
| description | Abstract Even though intracytoplasmic sperm injection (ICSI) has been widely used for the production of offspring in human infertility clinics and in reproductive research laboratories using mice, many researchers engaged in animal transgenesis still consider it somewhat cumbersome. The greatest advantage of ICSI-mediated transgenesis is that it allows introduction of very large DNA transgenes (e.g., yeast artificial chromosomes), with relatively high efficiency into the genomes of hosts, as compared to pronuclear injection. Recently, we have developed an active form of intracytoplasmic sperm injection-mediated transgenesis (ICSI-Tr) with fresh sperm utilizing transposons. The transgenic efficiencies rival all transgenic techniques except that of lentiviral methods. |
| doi_str_mv | 10.1016/j.cimid.2008.05.003 |
| format | Article |
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The greatest advantage of ICSI-mediated transgenesis is that it allows introduction of very large DNA transgenes (e.g., yeast artificial chromosomes), with relatively high efficiency into the genomes of hosts, as compared to pronuclear injection. Recently, we have developed an active form of intracytoplasmic sperm injection-mediated transgenesis (ICSI-Tr) with fresh sperm utilizing transposons. The transgenic efficiencies rival all transgenic techniques except that of lentiviral methods.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2008.05.003</identifier><identifier>PMID: 18691759</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Allergy and Immunology ; Animals ; Animals, Genetically Modified - genetics ; DNA Transposable Elements - genetics ; Female ; Fertilization in Vitro - methods ; Gene Transfer Techniques ; Genetic Vectors ; Humans ; Infectious Disease ; Male ; Mice ; Oocytes - physiology ; piggyBac ; Recombinant Proteins - genetics ; Sperm Injections, Intracytoplasmic - methods ; Spermatozoa - physiology ; Transgenes - genetics ; Transgenesis ; Transgénèse ; Transposase ; Transposases - genetics ; Transposon</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2009-03, Vol.32 (2), p.47-60</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><rights>2008 Elsevier Ltd. All rights reserved. 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c609t-284449ff7fedd2c608714d518fbe61495725dbb4eb6e232c47f544039459d3533</citedby><cites>FETCH-LOGICAL-c609t-284449ff7fedd2c608714d518fbe61495725dbb4eb6e232c47f544039459d3533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cimid.2008.05.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18691759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moisyadi, Stefan</creatorcontrib><creatorcontrib>Kaminski, Joseph M</creatorcontrib><creatorcontrib>Yanagimachi, Ryuzo</creatorcontrib><title>Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description>Abstract Even though intracytoplasmic sperm injection (ICSI) has been widely used for the production of offspring in human infertility clinics and in reproductive research laboratories using mice, many researchers engaged in animal transgenesis still consider it somewhat cumbersome. The greatest advantage of ICSI-mediated transgenesis is that it allows introduction of very large DNA transgenes (e.g., yeast artificial chromosomes), with relatively high efficiency into the genomes of hosts, as compared to pronuclear injection. Recently, we have developed an active form of intracytoplasmic sperm injection-mediated transgenesis (ICSI-Tr) with fresh sperm utilizing transposons. The transgenic efficiencies rival all transgenic techniques except that of lentiviral methods.</description><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Animals, Genetically Modified - genetics</subject><subject>DNA Transposable Elements - genetics</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Vectors</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Male</subject><subject>Mice</subject><subject>Oocytes - physiology</subject><subject>piggyBac</subject><subject>Recombinant Proteins - genetics</subject><subject>Sperm Injections, Intracytoplasmic - methods</subject><subject>Spermatozoa - physiology</subject><subject>Transgenes - genetics</subject><subject>Transgenesis</subject><subject>Transgénèse</subject><subject>Transposase</subject><subject>Transposases - genetics</subject><subject>Transposon</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhi1ERZfCL0BCOSF6SPBXbOdAJbQqsFIRh1KJm5U4k-KQxFs7W2n_PRN2xUcvPY08ft_xjJ8h5BWjBaNMvesL50ffFpxSU9CyoFQ8IStmtMmZUvopWVEmdV6Vmp2S5yn1lNKKSfaMnDKjKqbLakW-3CTIQpf5aY61289hO9Rp9C5LW4gjpntwsw9T9nazvt6cZ3PIbmGCWM-QoWNKeEJ1PfmxHtILctJhgJfHeEZuPl5-W3_Or75-2qw_XOVO0WrOuZFSVl2nO2hbjjmjmWxLZroGFJPYMS_bppHQKOCCO6m7UkoqKllWrSiFOCMXh7rbXTNC62DpfrDbiF3EvQ21t__fTP6HvQ33VkhuOGdY4M2xQAx3O0izHX1yMAz1BGGXrFLGCM34o0JOBQ5TaRSKg9DFkFKE7k83jNqFl-3tb1524WVpaZEXul7_O8hfzxEQCt4fBIDfee8h2uQ8TA5aH5GMbYN_5IGLB343eCRWDz9hD6kPuzghKcts4pba62Vllo2hBreFq-_iF82qvNQ</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Moisyadi, Stefan</creator><creator>Kaminski, Joseph M</creator><creator>Yanagimachi, Ryuzo</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090301</creationdate><title>Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals</title><author>Moisyadi, Stefan ; Kaminski, Joseph M ; Yanagimachi, Ryuzo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c609t-284449ff7fedd2c608714d518fbe61495725dbb4eb6e232c47f544039459d3533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Animals, Genetically Modified - genetics</topic><topic>DNA Transposable Elements - genetics</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Vectors</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Male</topic><topic>Mice</topic><topic>Oocytes - physiology</topic><topic>piggyBac</topic><topic>Recombinant Proteins - genetics</topic><topic>Sperm Injections, Intracytoplasmic - methods</topic><topic>Spermatozoa - physiology</topic><topic>Transgenes - genetics</topic><topic>Transgenesis</topic><topic>Transgénèse</topic><topic>Transposase</topic><topic>Transposases - genetics</topic><topic>Transposon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moisyadi, Stefan</creatorcontrib><creatorcontrib>Kaminski, Joseph M</creatorcontrib><creatorcontrib>Yanagimachi, Ryuzo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moisyadi, Stefan</au><au>Kaminski, Joseph M</au><au>Yanagimachi, Ryuzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>32</volume><issue>2</issue><spage>47</spage><epage>60</epage><pages>47-60</pages><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>Abstract Even though intracytoplasmic sperm injection (ICSI) has been widely used for the production of offspring in human infertility clinics and in reproductive research laboratories using mice, many researchers engaged in animal transgenesis still consider it somewhat cumbersome. 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| identifier | ISSN: 0147-9571 |
| ispartof | Comparative immunology, microbiology and infectious diseases, 2009-03, Vol.32 (2), p.47-60 |
| issn | 0147-9571 1878-1667 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Allergy and Immunology Animals Animals, Genetically Modified - genetics DNA Transposable Elements - genetics Female Fertilization in Vitro - methods Gene Transfer Techniques Genetic Vectors Humans Infectious Disease Male Mice Oocytes - physiology piggyBac Recombinant Proteins - genetics Sperm Injections, Intracytoplasmic - methods Spermatozoa - physiology Transgenes - genetics Transgenesis Transgénèse Transposase Transposases - genetics Transposon |
| title | Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals |
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