Preferential binding of S phase proteins to temporally characteristic units of replication in Physarum polycephalum
Synchronous plasmodia of Physarum polycephalum were pulse-labeled with 3H-thymidine in early or late portions of the S-phase, and the binding capacity of the replicated DNA for isochronous S-phase plasmodial proteins assessed by nitrocellulose filter binding assay. Replication units replicating duri...
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| Veröffentlicht in: | Nucleic acids research 1977-09, Vol.4 (9), p.3143-3154 |
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| creator | Wille, J.J. Jr |
| description | Synchronous plasmodia of Physarum polycephalum were pulse-labeled with 3H-thymidine in early or late portions of the S-phase, and the binding capacity of the replicated DNA for isochronous S-phase plasmodial proteins assessed by nitrocellulose filter binding assay. Replication units replicating during the first one-third of the S-phase preferentially bind cytosol proteins present in plasmodia engaged in early S DNA replication, while late S replicating DNA exhibits a corresponding preferential binding of plasmodial proteins present only in late S plasmodia. Temporally-characteristic nascent replication units were isolated by Hydroxylapatite column chromatography and were found to contain binding sites for isochronous proteins. |
| doi_str_mv | 10.1093/nar/4.9.3143 |
| format | Article |
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Temporally-characteristic nascent replication units were isolated by Hydroxylapatite column chromatography and were found to contain binding sites for isochronous proteins.</description><subject>Cytosol - metabolism</subject><subject>DNA - biosynthesis</subject><subject>DNA - isolation & purification</subject><subject>DNA Replication</subject><subject>Fungal Proteins - biosynthesis</subject><subject>fungi</subject><subject>Histones</subject><subject>Kinetics</subject><subject>Mitosis</subject><subject>Molecular Weight</subject><subject>Myxomycetes - metabolism</subject><subject>Physarum - metabolism</subject><subject>Physarum polycephalum</subject><subject>Protein Binding</subject><subject>Time Factors</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiPE11K4cUTCJ05ka3scJz70gCpKEZVYqVup4mI5jrNrSOxgO4j993iVagUnTpb1PjOamacoXhO8JljAuVPhnK3FGgiDR8WKAKclE5w-LlYYcFUSzJrnxYsYv2NMGKnYs-JpxYmoyKqIm2B6E4xLVg2ota6zbod8j27RtFfRoCn4ZKyLKHmUzDj5oIbhgPReBaWTCTYmq9HsbIrHsmCmwWqVrHfIOrTZH6IK84gmPxy0yS2HeXxZPOnVEM2rh_esuLv6uL28Lm--fvp8-eGm1IzWqSSgGwwceE-hU01raNu3HeaEdVTzvhNdDV3-16InAFxjijm0ShCsDIHWwFlxsfSd5nY0nc5L5uHlFOyowkF6ZeW_ibN7ufO_JDDKGc717x7qg_85m5jkaKM2w6Cc8XOUNXBe0Vr8FySCiaaueAbfL6AOPsZ8-dMwBMujS5ldSiaFPLrM-Ju_FzjBi7wcl0ucHZjfp1SFH5LXUFfy-v6b3Fzharut7-WXzL9d-F55qXZZnby7pZgApqIhjAv4A487tsE</recordid><startdate>197709</startdate><enddate>197709</enddate><creator>Wille, J.J. 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Replication units replicating during the first one-third of the S-phase preferentially bind cytosol proteins present in plasmodia engaged in early S DNA replication, while late S replicating DNA exhibits a corresponding preferential binding of plasmodial proteins present only in late S plasmodia. Temporally-characteristic nascent replication units were isolated by Hydroxylapatite column chromatography and were found to contain binding sites for isochronous proteins.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>561951</pmid><doi>10.1093/nar/4.9.3143</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1977-09, Vol.4 (9), p.3143-3154 |
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| language | eng |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central |
| subjects | Cytosol - metabolism DNA - biosynthesis DNA - isolation & purification DNA Replication Fungal Proteins - biosynthesis fungi Histones Kinetics Mitosis Molecular Weight Myxomycetes - metabolism Physarum - metabolism Physarum polycephalum Protein Binding Time Factors |
| title | Preferential binding of S phase proteins to temporally characteristic units of replication in Physarum polycephalum |
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