Changes in chromatin structure induced by EDTA treatment and partial removal of histone H1

Changes in chromatin structure induced by EDTA treatment and partial removal of histone H1

Electron microscopy shows that EDTA treatment or partial removal of histone HI converts 200–250 Å chromatin fibres characteristic for native chromatin, isolated in low ionic strength conditions into fibres consisting of nucleosomes connected by segments of DNA. This structural transition is accompan...

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Veröffentlicht in:Nucleic acids research 1977-09, Vol.4 (9), p.3017-3028
Hauptverfasser: Vengerov, Yu. Yu, Popenko, V. I.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
Chromatin - drug effects
Chromatin - ultrastructure
Circular Dichroism
DNA
Edetic Acid
Histones
Microscopy, Electron
Osmolar Concentration
Thymus Gland - ultrastructure
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creator Vengerov, Yu. Yu
Popenko, V. I.
description Electron microscopy shows that EDTA treatment or partial removal of histone HI converts 200–250 Å chromatin fibres characteristic for native chromatin, isolated in low ionic strength conditions into fibres consisting of nucleosomes connected by segments of DNA. This structural transition is accompanied by an increase in the amplitude of positive band of CD spectra at 280 nm. Comparison of electron microscopic, thermal denaturation and electrophoretic data suggests that multiphasic character of melting curves, observed for chromatin, lacking histone HI is due to the removal of histone HI and destabilisation of the DNA segments, connecting nucleosomes. It is also shown that bivalent cations play an important part both in the stabilisation of 200 Å globules and of nucleosomes.
doi_str_mv 10.1093/nar/4.9.3017
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subjects Animals
Cattle
Chromatin - drug effects
Chromatin - ultrastructure
Circular Dichroism
DNA
Edetic Acid
Histones
Microscopy, Electron
Osmolar Concentration
Thymus Gland - ultrastructure
title Changes in chromatin structure induced by EDTA treatment and partial removal of histone H1
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