Neurosteroid Analog Photolabeling of a Site in the Third Transmembrane Domain of the β3 Subunit of the GABAA Receptor

Accumulated evidence suggests that neurosteroids modulate GABAA receptors through binding interactions with transmembrane domains. To identify these neurosteroid binding sites directly, a neurosteroid-analog photolabeling reagent, (3α,5β)-6-azi-pregnanolone (6-AziP), was used to photolabel membranes...

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Veröffentlicht in:	Molecular pharmacology 2012-09, Vol.82 (3), p.408-419
Hauptverfasser:	Chen, Zi-Wei, Manion, Brad, Townsend, R. Reid, Reichert, David E., Covey, Douglas F., Steinbach, Joe Henry, Sieghart, Werner, Fuchs, Karoline, Evers, Alex S.
Format:	Artikel
Sprache:	eng
Schlagworte:	6-azi-pregnanolone 6-AziP acetonitrile ACN directed product-ion spectral acquisition DMSA E-64 extracted ion chromatogram formic acid Glu-Cl glutamate-gated chloride channel linear quadrupole ion trap liquid chromatography LTQ mass spectrometry MS1 MS2 N-[N-(l-3-trans-carboxyirane-2-carbonyl)-l-leucyl]-agmatine PAGE PDB PGC polyacrylamide gel electrophoresis porous graphitic carbon precursor-ion mass spectrometry product-ion mass spectrometry Protein Data Bank solid-phase extraction SPE t-butyl-bicyclophosphorothionate TBPS TMD transformed human embryonic kidney transmembrane domain transmembrane region TSA VDAC voltage-dependent anion channel XIC
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