Tracking genome engineering outcome at individual DNA breakpoints

A fluorescent reporter, named traffic light, reads out whether repair of a DNA break occurs by nonhomologous end-joining or by homologous recombination. It should enable the identification of factors that affect repair pathway choice and thus improved approaches for genome engineering. Site-specific...

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Veröffentlicht in:	Nature methods 2011-08, Vol.8 (8), p.671-676
Hauptverfasser:	Certo, Michael T, Ryu, Byoung Y, Annis, James E, Garibov, Mikhail, Jarjour, Jordan, Rawlings, David J, Scharenberg, Andrew M
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1513/1967 631/1647/338 631/1647/338/552 631/337/1427 Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biotechnology Cellular biology Deoxyribonucleic acid DNA DNA damage DNA Damage - genetics DNA repair DNA Repair - genetics Genes, Reporter - genetics Genetic engineering Genetic Engineering - methods Genome - genetics Genomics Life Sciences Proteomics Rapid flow
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