Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells
Mesenchymal-epithelial interactions play an important role in the physiology and pathology of epithelial tissues. Mesenchymal cells either associate with epithelium basement membrane [pericytes and perivascular monocyte-derived cells (MDC)] or reside within epithelium (MDC and T cells). Although int...
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| Veröffentlicht in: | BMC developmental biology 2001-06, Vol.1 (1), p.11-11, Article 11 |
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| creator | Bukovsky, A Caudle, M R Keenan, J A Upadhyaya, N B Van Meter, S E Wimalasena, J Elder, R F |
| description | Mesenchymal-epithelial interactions play an important role in the physiology and pathology of epithelial tissues. Mesenchymal cells either associate with epithelium basement membrane [pericytes and perivascular monocyte-derived cells (MDC)] or reside within epithelium (MDC and T cells). Although intraepithelial mesenchymal cells were suggested to contribute to the epithelium physiology, their association with particular steps in differentiation of epithelial cells, interactions among themselves, and their fate remain unclear. We studied epitopes of mesenchymal cells and their products (immunoglobulins) in stratified epithelium of uterine ectocervix, which is one of the prototypes of complete cellular differentiation from stem into the aged cells.
Perivascular CD14 primitive MDC associated with basal (stem) epithelial cells. Thy-1 pericytes of microvasculature secreted intercellular vesicles, which associated with Ki67 postmitotic epithelial cells expressing MHC class I. Intraepithelial T cells showed an association with veiled type MDC [dendritic cell (DC) precursors] among parabasal cells, and exhibited fragmentation after entering intermediate (mature) epithelial layers. Mature DC secreted CD68 and exhibited fragmentation after reaching mid intermediate layers. Binding of IgM was detected at the top of each layer: in the upper parabasal, upper intermediate, and most surface epithelial cells. IgG was confined to the entire superficial layer.
These data suggest that the phylogenetically and ontogenetically developed hierarchy of mesenchymal cells (MDC, pericytes, T cells) and immunoglobulins (IgM, IgG) accompanies differentiation of epithelial cells from immature into the mature and aged phenotype. Further studies of an involvement of mesenchymal cells in the regulation of tissue homeostasis may bring novel approaches to the prevention and therapy of tissue dysfunctions characterized by permanent tissue immaturity (muscular dystrophy) or accelerated aging (degenerative diseases). |
| doi_str_mv | 10.1186/1471-213X-1-11 |
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Perivascular CD14 primitive MDC associated with basal (stem) epithelial cells. Thy-1 pericytes of microvasculature secreted intercellular vesicles, which associated with Ki67 postmitotic epithelial cells expressing MHC class I. Intraepithelial T cells showed an association with veiled type MDC [dendritic cell (DC) precursors] among parabasal cells, and exhibited fragmentation after entering intermediate (mature) epithelial layers. Mature DC secreted CD68 and exhibited fragmentation after reaching mid intermediate layers. Binding of IgM was detected at the top of each layer: in the upper parabasal, upper intermediate, and most surface epithelial cells. IgG was confined to the entire superficial layer.
These data suggest that the phylogenetically and ontogenetically developed hierarchy of mesenchymal cells (MDC, pericytes, T cells) and immunoglobulins (IgM, IgG) accompanies differentiation of epithelial cells from immature into the mature and aged phenotype. Further studies of an involvement of mesenchymal cells in the regulation of tissue homeostasis may bring novel approaches to the prevention and therapy of tissue dysfunctions characterized by permanent tissue immaturity (muscular dystrophy) or accelerated aging (degenerative diseases).</description><identifier>ISSN: 1471-213X</identifier><identifier>EISSN: 1471-213X</identifier><identifier>DOI: 10.1186/1471-213X-1-11</identifier><identifier>PMID: 11439174</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Antigenic determinants ; Apoptosis ; Apoptosis - physiology ; CD3 Complex - analysis ; CD8 Antigens - analysis ; Cell differentiation ; Cell Differentiation - physiology ; Cervix Uteri - blood supply ; Cervix Uteri - cytology ; Dendritic cells ; Epithelial Cells - cytology ; Epithelial Cells - physiology ; Epithelium - blood supply ; Epithelium - physiology ; Female ; Health aspects ; Humans ; Immunoglobulin G ; Immunoglobulins ; Immunoglobulins - blood ; Immunoglobulins - physiology ; Immunohistochemistry ; Mesoderm - cytology ; Mesoderm - physiology ; Physiological aspects ; Stem cells ; T cells</subject><ispartof>BMC developmental biology, 2001-06, Vol.1 (1), p.11-11, Article 11</ispartof><rights>COPYRIGHT 2001 BioMed Central Ltd.</rights><rights>Copyright © 2001 Bukovsky et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. 2001 Bukovsky et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b5981-b4ce493b76150998037282d5c6c62afbdbd361165b3ba048232e03899da300563</citedby><cites>FETCH-LOGICAL-b5981-b4ce493b76150998037282d5c6c62afbdbd361165b3ba048232e03899da300563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC34117/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC34117/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,24799,27922,27923,53789,53791,75508,75509</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11439174$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bukovsky, A</creatorcontrib><creatorcontrib>Caudle, M R</creatorcontrib><creatorcontrib>Keenan, J A</creatorcontrib><creatorcontrib>Upadhyaya, N B</creatorcontrib><creatorcontrib>Van Meter, S E</creatorcontrib><creatorcontrib>Wimalasena, J</creatorcontrib><creatorcontrib>Elder, R F</creatorcontrib><title>Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells</title><title>BMC developmental biology</title><addtitle>BMC Dev Biol</addtitle><description>Mesenchymal-epithelial interactions play an important role in the physiology and pathology of epithelial tissues. Mesenchymal cells either associate with epithelium basement membrane [pericytes and perivascular monocyte-derived cells (MDC)] or reside within epithelium (MDC and T cells). Although intraepithelial mesenchymal cells were suggested to contribute to the epithelium physiology, their association with particular steps in differentiation of epithelial cells, interactions among themselves, and their fate remain unclear. We studied epitopes of mesenchymal cells and their products (immunoglobulins) in stratified epithelium of uterine ectocervix, which is one of the prototypes of complete cellular differentiation from stem into the aged cells.
Perivascular CD14 primitive MDC associated with basal (stem) epithelial cells. Thy-1 pericytes of microvasculature secreted intercellular vesicles, which associated with Ki67 postmitotic epithelial cells expressing MHC class I. Intraepithelial T cells showed an association with veiled type MDC [dendritic cell (DC) precursors] among parabasal cells, and exhibited fragmentation after entering intermediate (mature) epithelial layers. Mature DC secreted CD68 and exhibited fragmentation after reaching mid intermediate layers. Binding of IgM was detected at the top of each layer: in the upper parabasal, upper intermediate, and most surface epithelial cells. IgG was confined to the entire superficial layer.
These data suggest that the phylogenetically and ontogenetically developed hierarchy of mesenchymal cells (MDC, pericytes, T cells) and immunoglobulins (IgM, IgG) accompanies differentiation of epithelial cells from immature into the mature and aged phenotype. Further studies of an involvement of mesenchymal cells in the regulation of tissue homeostasis may bring novel approaches to the prevention and therapy of tissue dysfunctions characterized by permanent tissue immaturity (muscular dystrophy) or accelerated aging (degenerative diseases).</description><subject>Analysis</subject><subject>Antigenic determinants</subject><subject>Apoptosis</subject><subject>Apoptosis - physiology</subject><subject>CD3 Complex - analysis</subject><subject>CD8 Antigens - analysis</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - physiology</subject><subject>Cervix Uteri - blood supply</subject><subject>Cervix Uteri - cytology</subject><subject>Dendritic cells</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - physiology</subject><subject>Epithelium - blood supply</subject><subject>Epithelium - physiology</subject><subject>Female</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulins</subject><subject>Immunoglobulins - blood</subject><subject>Immunoglobulins - physiology</subject><subject>Immunohistochemistry</subject><subject>Mesoderm - cytology</subject><subject>Mesoderm - physiology</subject><subject>Physiological aspects</subject><subject>Stem cells</subject><subject>T cells</subject><issn>1471-213X</issn><issn>1471-213X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFktuL1TAQxou4uOvqq49SEBQfumaaNGnBl8PiZWFB8ALiS0jSaU-kSY5Nq-5_b8o5eznsquQhYeb3DTNfJsueADkBqPkrYAKKEujXAgqAe9nRVeD-jfdh9jDG74SAqIE_yA4BGG1AsKPs2yrGYKyabPB56HKHEb1ZXzg15AaHIebKt7l1bvahH4KeB-tj_stO67y1XYcj-mlR-z7HTYriYC-Vj7KDTg0RH-_u4-zL2zefT98X5x_enZ2uzgtdNTUUmhlkDdWCQ0WapiZUlHXZVoYbXqpOt7qlHIBXmmpFWF3SEgmtm6ZVlJCK0-Ps9bbuZtYOW5M6GtUgN6N1aryQQVm5n_F2LfvwU1IGIJJ8tZVrG_4i38-Y4OTirFyclSABUo0XuxbG8GPGOEln42KC8hjmKAXjyXwqSCKf_5tMFAMG_wWhYaSpYBn_2Rbs1YDS-i6kLs0Cy1VNSi6qmi5TntxBpdOisyZ47GyK7wle7gkSM-HvqVdzjPLs08c7i5sxxDhid-UeELms6W2_nt78smt8t5f0DwdT4m0</recordid><startdate>20010622</startdate><enddate>20010622</enddate><creator>Bukovsky, A</creator><creator>Caudle, M R</creator><creator>Keenan, J A</creator><creator>Upadhyaya, N B</creator><creator>Van Meter, S E</creator><creator>Wimalasena, J</creator><creator>Elder, R F</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010622</creationdate><title>Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells</title><author>Bukovsky, A ; Caudle, M R ; Keenan, J A ; Upadhyaya, N B ; Van Meter, S E ; Wimalasena, J ; Elder, R F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b5981-b4ce493b76150998037282d5c6c62afbdbd361165b3ba048232e03899da300563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Analysis</topic><topic>Antigenic determinants</topic><topic>Apoptosis</topic><topic>Apoptosis - physiology</topic><topic>CD3 Complex - analysis</topic><topic>CD8 Antigens - analysis</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - physiology</topic><topic>Cervix Uteri - blood supply</topic><topic>Cervix Uteri - cytology</topic><topic>Dendritic cells</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - physiology</topic><topic>Epithelium - blood supply</topic><topic>Epithelium - physiology</topic><topic>Female</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulins</topic><topic>Immunoglobulins - blood</topic><topic>Immunoglobulins - physiology</topic><topic>Immunohistochemistry</topic><topic>Mesoderm - cytology</topic><topic>Mesoderm - physiology</topic><topic>Physiological aspects</topic><topic>Stem cells</topic><topic>T cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bukovsky, A</creatorcontrib><creatorcontrib>Caudle, M R</creatorcontrib><creatorcontrib>Keenan, J A</creatorcontrib><creatorcontrib>Upadhyaya, N B</creatorcontrib><creatorcontrib>Van Meter, S E</creatorcontrib><creatorcontrib>Wimalasena, J</creatorcontrib><creatorcontrib>Elder, R F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bukovsky, A</au><au>Caudle, M R</au><au>Keenan, J A</au><au>Upadhyaya, N B</au><au>Van Meter, S E</au><au>Wimalasena, J</au><au>Elder, R F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells</atitle><jtitle>BMC developmental biology</jtitle><addtitle>BMC Dev Biol</addtitle><date>2001-06-22</date><risdate>2001</risdate><volume>1</volume><issue>1</issue><spage>11</spage><epage>11</epage><pages>11-11</pages><artnum>11</artnum><issn>1471-213X</issn><eissn>1471-213X</eissn><abstract>Mesenchymal-epithelial interactions play an important role in the physiology and pathology of epithelial tissues. Mesenchymal cells either associate with epithelium basement membrane [pericytes and perivascular monocyte-derived cells (MDC)] or reside within epithelium (MDC and T cells). Although intraepithelial mesenchymal cells were suggested to contribute to the epithelium physiology, their association with particular steps in differentiation of epithelial cells, interactions among themselves, and their fate remain unclear. We studied epitopes of mesenchymal cells and their products (immunoglobulins) in stratified epithelium of uterine ectocervix, which is one of the prototypes of complete cellular differentiation from stem into the aged cells.
Perivascular CD14 primitive MDC associated with basal (stem) epithelial cells. Thy-1 pericytes of microvasculature secreted intercellular vesicles, which associated with Ki67 postmitotic epithelial cells expressing MHC class I. Intraepithelial T cells showed an association with veiled type MDC [dendritic cell (DC) precursors] among parabasal cells, and exhibited fragmentation after entering intermediate (mature) epithelial layers. Mature DC secreted CD68 and exhibited fragmentation after reaching mid intermediate layers. Binding of IgM was detected at the top of each layer: in the upper parabasal, upper intermediate, and most surface epithelial cells. IgG was confined to the entire superficial layer.
These data suggest that the phylogenetically and ontogenetically developed hierarchy of mesenchymal cells (MDC, pericytes, T cells) and immunoglobulins (IgM, IgG) accompanies differentiation of epithelial cells from immature into the mature and aged phenotype. Further studies of an involvement of mesenchymal cells in the regulation of tissue homeostasis may bring novel approaches to the prevention and therapy of tissue dysfunctions characterized by permanent tissue immaturity (muscular dystrophy) or accelerated aging (degenerative diseases).</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>11439174</pmid><doi>10.1186/1471-213X-1-11</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Antigenic determinants Apoptosis Apoptosis - physiology CD3 Complex - analysis CD8 Antigens - analysis Cell differentiation Cell Differentiation - physiology Cervix Uteri - blood supply Cervix Uteri - cytology Dendritic cells Epithelial Cells - cytology Epithelial Cells - physiology Epithelium - blood supply Epithelium - physiology Female Health aspects Humans Immunoglobulin G Immunoglobulins Immunoglobulins - blood Immunoglobulins - physiology Immunohistochemistry Mesoderm - cytology Mesoderm - physiology Physiological aspects Stem cells T cells |
| title | Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells |
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