Biocompatibility and biofilm inhibition of N , N -hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials
Abstract The biocompatibility and antibacterial properties of N , N -hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibi...
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| Veröffentlicht in: | Biomaterials 2011-12, Vol.32 (34), p.8783-8796 |
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| creator | Behlau, Irmgard Mukherjee, Koushik Todani, Amit Tisdale, Ann S Cade, Fabiano Wang, Liqiang Leonard, Elizabeth M Zakka, Fouad R Gilmore, Michael S Jakobiec, Frederick A Dohlman, Claes H Klibanov, Alexander M |
| description | Abstract The biocompatibility and antibacterial properties of N , N -hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibitory effect on biofilm formation by Staphylococcus aureus clinical isolates, as compared to the parent poly(methyl methacrylate) (PMMA) and titanium. There was no additional corneal epithelial cell cytotoxicity of HMPEI-coated PMMA compared to that of control PMMA in tissue cultures in vitro . Likewise, no toxicity or adverse reactivity was detected with HMPEI-derivatized PMMA or titanium compared to those of the control materials after intrastromal or anterior chamber implantation in rabbits in vivo. |
| doi_str_mv | 10.1016/j.biomaterials.2011.08.010 |
| format | Article |
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By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibitory effect on biofilm formation by Staphylococcus aureus clinical isolates, as compared to the parent poly(methyl methacrylate) (PMMA) and titanium. There was no additional corneal epithelial cell cytotoxicity of HMPEI-coated PMMA compared to that of control PMMA in tissue cultures in vitro . Likewise, no toxicity or adverse reactivity was detected with HMPEI-derivatized PMMA or titanium compared to those of the control materials after intrastromal or anterior chamber implantation in rabbits in vivo.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2011.08.010</identifier><identifier>PMID: 21903257</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Antibacterial ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Biofilms - drug effects ; Cornea - cytology ; Cornea - drug effects ; Corneal cytotoxicity ; Dentistry ; Keratoprosthesis ; PMMA ; Polyethyleneimine - chemistry ; Polyethyleneimine - pharmacology ; Polyethylenimine (PEI) ; Polymethyl Methacrylate - chemistry ; Polymethyl Methacrylate - pharmacology ; Prostheses and Implants ; Rabbits ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - physiology ; Titanium ; Titanium - chemistry ; Titanium - pharmacology</subject><ispartof>Biomaterials, 2011-12, Vol.32 (34), p.8783-8796</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><rights>2011 Elsevier Ltd. All rights reserved. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-9d19d7e51ac0fd6300b6c5c28e516e5635bf7fa8acd2c12f90ae20c6ac6655ea3</citedby><cites>FETCH-LOGICAL-c573t-9d19d7e51ac0fd6300b6c5c28e516e5635bf7fa8acd2c12f90ae20c6ac6655ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2011.08.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21903257$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Behlau, Irmgard</creatorcontrib><creatorcontrib>Mukherjee, Koushik</creatorcontrib><creatorcontrib>Todani, Amit</creatorcontrib><creatorcontrib>Tisdale, Ann S</creatorcontrib><creatorcontrib>Cade, Fabiano</creatorcontrib><creatorcontrib>Wang, Liqiang</creatorcontrib><creatorcontrib>Leonard, Elizabeth M</creatorcontrib><creatorcontrib>Zakka, Fouad R</creatorcontrib><creatorcontrib>Gilmore, Michael S</creatorcontrib><creatorcontrib>Jakobiec, Frederick A</creatorcontrib><creatorcontrib>Dohlman, Claes H</creatorcontrib><creatorcontrib>Klibanov, Alexander M</creatorcontrib><title>Biocompatibility and biofilm inhibition of N , N -hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract The biocompatibility and antibacterial properties of N , N -hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibitory effect on biofilm formation by Staphylococcus aureus clinical isolates, as compared to the parent poly(methyl methacrylate) (PMMA) and titanium. There was no additional corneal epithelial cell cytotoxicity of HMPEI-coated PMMA compared to that of control PMMA in tissue cultures in vitro . Likewise, no toxicity or adverse reactivity was detected with HMPEI-derivatized PMMA or titanium compared to those of the control materials after intrastromal or anterior chamber implantation in rabbits in vivo.</description><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biofilms - drug effects</subject><subject>Cornea - cytology</subject><subject>Cornea - drug effects</subject><subject>Corneal cytotoxicity</subject><subject>Dentistry</subject><subject>Keratoprosthesis</subject><subject>PMMA</subject><subject>Polyethyleneimine - chemistry</subject><subject>Polyethyleneimine - pharmacology</subject><subject>Polyethylenimine (PEI)</subject><subject>Polymethyl Methacrylate - chemistry</subject><subject>Polymethyl Methacrylate - pharmacology</subject><subject>Prostheses and Implants</subject><subject>Rabbits</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - physiology</subject><subject>Titanium</subject><subject>Titanium - chemistry</subject><subject>Titanium - pharmacology</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUk1v1DAQjRCIlsJfQBEXLs0y46zzwaESLZ-iggNwthxnws6S2FvbWxHx5_GyZVW4wMEaz_i95_E8Z9kThAUCVs_Wi47dpCN51mNYCEBcQLMAhDvZMTZ1U8gW5N3sGHApirZCcZQ9CGENKYeluJ8dCWyhFLI-zn6cszNu2ujIHY8c51zbPk_6A49TznaVypGdzd2Qf8hP0ypW9H0eTyeKq3ksNm6cf-3I8sSW8s7Znvo8uvzchZiI78nr6DY-ZSsKHPJD5w-ze0MK9OgmnmRfXr_6fPG2uPz45t3Fi8vCyLqMRdtj29ckURsY-qoE6CojjWhSqSJZlbIb6kE32vTCoBha0CTAVNpUlZSky5PsbK-72XYT9YZs9HpUG8-T9rNymtWfJ5ZX6qu7VuUSoS0xCTy9EfDuakshqomDoXHUltw2qBZE2WCN_0Y2rWykqOoyIZ_vkSZNJngaDv0gqJ3Naq1u26x2NitoVLI5kR_fftGB-tvXBHi5B1Ca6zWTV8EwWUM9ezJR9Y7_756zv2TMyJaNHr_RTGHttt7uOKiCUKA-7T7c7r8hArS4bMqfp4PZLQ</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Behlau, Irmgard</creator><creator>Mukherjee, Koushik</creator><creator>Todani, Amit</creator><creator>Tisdale, Ann S</creator><creator>Cade, Fabiano</creator><creator>Wang, Liqiang</creator><creator>Leonard, Elizabeth M</creator><creator>Zakka, Fouad R</creator><creator>Gilmore, Michael S</creator><creator>Jakobiec, Frederick A</creator><creator>Dohlman, Claes H</creator><creator>Klibanov, Alexander M</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20111201</creationdate><title>Biocompatibility and biofilm inhibition of N , N -hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials</title><author>Behlau, Irmgard ; Mukherjee, Koushik ; Todani, Amit ; Tisdale, Ann S ; Cade, Fabiano ; Wang, Liqiang ; Leonard, Elizabeth M ; Zakka, Fouad R ; Gilmore, Michael S ; Jakobiec, Frederick A ; Dohlman, Claes H ; Klibanov, Alexander M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-9d19d7e51ac0fd6300b6c5c28e516e5635bf7fa8acd2c12f90ae20c6ac6655ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biofilms - drug effects</topic><topic>Cornea - cytology</topic><topic>Cornea - drug effects</topic><topic>Corneal cytotoxicity</topic><topic>Dentistry</topic><topic>Keratoprosthesis</topic><topic>PMMA</topic><topic>Polyethyleneimine - chemistry</topic><topic>Polyethyleneimine - pharmacology</topic><topic>Polyethylenimine (PEI)</topic><topic>Polymethyl Methacrylate - chemistry</topic><topic>Polymethyl Methacrylate - pharmacology</topic><topic>Prostheses and Implants</topic><topic>Rabbits</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - physiology</topic><topic>Titanium</topic><topic>Titanium - chemistry</topic><topic>Titanium - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Behlau, Irmgard</creatorcontrib><creatorcontrib>Mukherjee, Koushik</creatorcontrib><creatorcontrib>Todani, Amit</creatorcontrib><creatorcontrib>Tisdale, Ann S</creatorcontrib><creatorcontrib>Cade, Fabiano</creatorcontrib><creatorcontrib>Wang, Liqiang</creatorcontrib><creatorcontrib>Leonard, Elizabeth M</creatorcontrib><creatorcontrib>Zakka, Fouad R</creatorcontrib><creatorcontrib>Gilmore, Michael S</creatorcontrib><creatorcontrib>Jakobiec, Frederick A</creatorcontrib><creatorcontrib>Dohlman, Claes H</creatorcontrib><creatorcontrib>Klibanov, Alexander M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Behlau, Irmgard</au><au>Mukherjee, Koushik</au><au>Todani, Amit</au><au>Tisdale, Ann S</au><au>Cade, Fabiano</au><au>Wang, Liqiang</au><au>Leonard, Elizabeth M</au><au>Zakka, Fouad R</au><au>Gilmore, Michael S</au><au>Jakobiec, Frederick A</au><au>Dohlman, Claes H</au><au>Klibanov, Alexander M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biocompatibility and biofilm inhibition of N , N -hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>32</volume><issue>34</issue><spage>8783</spage><epage>8796</epage><pages>8783-8796</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract The biocompatibility and antibacterial properties of N , N -hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. 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| subjects | Advanced Basic Science Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibacterial Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Biofilms - drug effects Cornea - cytology Cornea - drug effects Corneal cytotoxicity Dentistry Keratoprosthesis PMMA Polyethyleneimine - chemistry Polyethyleneimine - pharmacology Polyethylenimine (PEI) Polymethyl Methacrylate - chemistry Polymethyl Methacrylate - pharmacology Prostheses and Implants Rabbits Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Titanium Titanium - chemistry Titanium - pharmacology |
| title | Biocompatibility and biofilm inhibition of N , N -hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials |
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