RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits: determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S5, S7, S8, S9, S11, S13, S19 and S21 by treatment with methyl p-azidophenyl acetimidate

RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by treatment with methyl p-azidophenyl acetimidate. After partial nuclease digestion of the RNA moiety, a number of cross-linked RNA-protein complexes were isolated by a new three-step procedure. Protein and RNA analysis of...

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Veröffentlicht in:	Nucleic acids research 1987-04, Vol.15 (8), p.3221-3240
Hauptverfasser:	OSSWALD, M, GREUER, B, BRIMACOMBE, R, STÖFFLER, G, BÄUMERT, H, FASOLD, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - analysis Bacteriology Base Sequence Biogenesis of cell structures, supramolecular organization Biological and medical sciences Cross-Linking Reagents Escherichia coli Escherichia coli - analysis Fundamental and applied biological sciences. Psychology Imidoesters Microbiology Nucleic Acid Conformation Ribosomal Proteins - analysis RNA, Bacterial - analysis RNA, Ribosomal - analysis
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