An in-house multiplex PCR test for the detection of Mycobacterium tuberculosis, its validation & comparison with a single target TB-PCR kit

The conventional techniques used in TB diagnosis like AFB (acid fast bacilli) smear microscopy lack sensitivity and the gold standard, culture test takes time. A test based on multiplex polymerase chain reaction (PCR) targeting the 38 kDa gene and IS6110 insertion sequence, specific to Mycobacterium...

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Veröffentlicht in:	Indian journal of medical research (New Delhi, India : 1994) India : 1994), 2012-05, Vol.135 (5), p.788-794
Hauptverfasser:	Kulkarni, Savita, Singh, P, Memon, Aafreen, Nataraj, Gita, Kanade, Swapna, Kelkar, Rohini, Rajan, M G R
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Bacterial - genetics Antigens, Bacterial - isolation & purification Bronchoalveolar Lavage Fluid - microbiology DNA, Bacterial - analysis Health aspects Humans Identification and classification Lipoproteins - genetics Lipoproteins - isolation & purification Multiplex Polymerase Chain Reaction - methods Mycobacterium tuberculosis Mycobacterium tuberculosis - isolation & purification Original Physiological aspects Polymerase chain reaction Sensitivity and Specificity Sputum - microbiology Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - microbiology
Online-Zugang:	Volltext
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