Edaravone inhibits apoptosis caused by ischemia/ reperfusion injury in a porcine hepatectomy model
AIM： TO investigate the effect of E3-methyl-l-phe- nyl-2-pyrazolin-5-one （Edr） on hepatic ischemia-reper- fusion （I/R） injury and liver regeneration in a porcine hepatectomy model. METHODS： One hour ischemia was induced by occlud- ing the vessels and the bile duct of the right and median lobes. A 40...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2012-07, Vol.18 (27), p.3520-3526 |
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| creator | Shimoda, Mitsugi Iwasaki, Yoshimi Okada, Toshie Kubota, Keiichi |
| description | AIM： TO investigate the effect of E3-methyl-l-phe- nyl-2-pyrazolin-5-one （Edr） on hepatic ischemia-reper- fusion （I/R） injury and liver regeneration in a porcine hepatectomy model. METHODS： One hour ischemia was induced by occlud- ing the vessels and the bile duct of the right and median lobes. A 40% left hepatectomy was performed after re- perfusion. Six animals received Edr （3 mg/kg per hour） intravenously and six control animals received saline just before reperfusion. Remnant liver volume, hemody- namics, aspartate aminotransferase （AST）, alanine ami- notransferase, lactate dehydrogenase and lactic acid, were compared between the groups. The expression of transforming growth factor-β （TGF-β1） and toll-like receptor （TRL） mRNA in hepatic tissues was examined using reverse transcription polymerase chain reaction. Apoptosis was demonstrated by terminal deoxynucleo- tidyl transferase dUTP nick end labeling （TUNEL） stain- ing, respectively. RESULTS： Serum AS-I- （P = 0.029）, and toll like recep- tor 4 level （P = 0.043） were significantly lower after 3 hin animals receiving Edr. In addition, TUNEL staining in Edr-treated pigs showed significantly fewer hepatocytes undergoing apoptosis compared with control pigs. After 1 mo, all factors were non-significantly different between the two groups. CONCLUSION： Edr is considered to reduce hepatic injury in the early stage of I/R injury in a porcine model. |
| doi_str_mv | 10.3748/wjg.v18.i27.3520 |
| format | Article |
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METHODS： One hour ischemia was induced by occlud- ing the vessels and the bile duct of the right and median lobes. A 40% left hepatectomy was performed after re- perfusion. Six animals received Edr （3 mg/kg per hour） intravenously and six control animals received saline just before reperfusion. Remnant liver volume, hemody- namics, aspartate aminotransferase （AST）, alanine ami- notransferase, lactate dehydrogenase and lactic acid, were compared between the groups. The expression of transforming growth factor-β （TGF-β1） and toll-like receptor （TRL） mRNA in hepatic tissues was examined using reverse transcription polymerase chain reaction. Apoptosis was demonstrated by terminal deoxynucleo- tidyl transferase dUTP nick end labeling （TUNEL） stain- ing, respectively. RESULTS： Serum AS-I- （P = 0.029）, and toll like recep- tor 4 level （P = 0.043） were significantly lower after 3 hin animals receiving Edr. In addition, TUNEL staining in Edr-treated pigs showed significantly fewer hepatocytes undergoing apoptosis compared with control pigs. After 1 mo, all factors were non-significantly different between the two groups. CONCLUSION： Edr is considered to reduce hepatic injury in the early stage of I/R injury in a porcine model.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v18.i27.3520</identifier><identifier>PMID: 22826616</identifier><language>eng</language><publisher>United States: Baishideng Publishing Group Co., Limited</publisher><subject>Animals ; Antipyrine - analogs & derivatives ; Antipyrine - pharmacology ; Apoptosis - drug effects ; Biomarkers - blood ; Cytoprotection ; Disease Models, Animal ; Free Radical Scavengers - pharmacology ; Gene Expression Regulation ; Hepatectomy - adverse effects ; In Situ Nick-End Labeling ; Liver - drug effects ; Liver - metabolism ; Liver - pathology ; Liver Regeneration - drug effects ; Male ; Original ; Real-Time Polymerase Chain Reaction ; Reperfusion Injury - etiology ; Reperfusion Injury - genetics ; Reperfusion Injury - metabolism ; Reperfusion Injury - pathology ; Reperfusion Injury - prevention & control ; Reverse Transcriptase Polymerase Chain Reaction ; Swine ; Time Factors ; Toll样受体 ; 依达拉奉 ; 切除术 ; 模型 ; 细胞凋亡 ; 缺血/再灌注损伤 ; 肝再生 ; 逆转录聚合酶链反应</subject><ispartof>World journal of gastroenterology : WJG, 2012-07, Vol.18 (27), p.3520-3526</ispartof><rights>2012 Baishideng Publishing Group Co., Limited. All rights reserved. 2012</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-e1315b7ed37c3133d2cf64cb5cf4bce3653182ed39f4e94c262ca00f4751015e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400853/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400853/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22826616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimoda, Mitsugi</creatorcontrib><creatorcontrib>Iwasaki, Yoshimi</creatorcontrib><creatorcontrib>Okada, Toshie</creatorcontrib><creatorcontrib>Kubota, Keiichi</creatorcontrib><title>Edaravone inhibits apoptosis caused by ischemia/ reperfusion injury in a porcine hepatectomy model</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM： TO investigate the effect of E3-methyl-l-phe- nyl-2-pyrazolin-5-one （Edr） on hepatic ischemia-reper- fusion （I/R） injury and liver regeneration in a porcine hepatectomy model. METHODS： One hour ischemia was induced by occlud- ing the vessels and the bile duct of the right and median lobes. A 40% left hepatectomy was performed after re- perfusion. Six animals received Edr （3 mg/kg per hour） intravenously and six control animals received saline just before reperfusion. Remnant liver volume, hemody- namics, aspartate aminotransferase （AST）, alanine ami- notransferase, lactate dehydrogenase and lactic acid, were compared between the groups. The expression of transforming growth factor-β （TGF-β1） and toll-like receptor （TRL） mRNA in hepatic tissues was examined using reverse transcription polymerase chain reaction. Apoptosis was demonstrated by terminal deoxynucleo- tidyl transferase dUTP nick end labeling （TUNEL） stain- ing, respectively. RESULTS： Serum AS-I- （P = 0.029）, and toll like recep- tor 4 level （P = 0.043） were significantly lower after 3 hin animals receiving Edr. In addition, TUNEL staining in Edr-treated pigs showed significantly fewer hepatocytes undergoing apoptosis compared with control pigs. After 1 mo, all factors were non-significantly different between the two groups. CONCLUSION： Edr is considered to reduce hepatic injury in the early stage of I/R injury in a porcine model.</description><subject>Animals</subject><subject>Antipyrine - analogs & derivatives</subject><subject>Antipyrine - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Biomarkers - blood</subject><subject>Cytoprotection</subject><subject>Disease Models, Animal</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Gene Expression Regulation</subject><subject>Hepatectomy - adverse effects</subject><subject>In Situ Nick-End Labeling</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Liver Regeneration - drug effects</subject><subject>Male</subject><subject>Original</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reperfusion Injury - etiology</subject><subject>Reperfusion Injury - genetics</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - pathology</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Swine</subject><subject>Time Factors</subject><subject>Toll样受体</subject><subject>依达拉奉</subject><subject>切除术</subject><subject>模型</subject><subject>细胞凋亡</subject><subject>缺血/再灌注损伤</subject><subject>肝再生</subject><subject>逆转录聚合酶链反应</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1v3CAURVHVqJkm3XdVubtuPIEHNnhTqYrSDylSN8kaYfw8ZmSDA_ZE8-9LlOmoXbG49x5Ah5CPjG65FOrmeb_bHpjaOpBbXgF9QzYArClBCfqWbBilsmw4yEvyPqU9pcBz6x25BFBQ16zekPauM9EcgsfC-cG1bkmFmcO8hORSYc2asCvaY-GSHXBy5qaIOGPs1-SCz5P9GnPoC1PMIVqXMQPOZkG7hOlYTKHD8Zpc9GZM-OF0XpHH73cPtz_L-98_ft1-uy-tAFhKZJxVrcSOS8sZ5x3Yvha2rWwvWou8rjhTkOOmF9gICzVYQ2kvZMUoq5Bfka-v3HltJ-ws-iWaUc_RTSYedTBO_594N-hdOGguKFUVz4AvJ0AMTyumRU_52ziOxmNYk2YUpOKibmSu0teqjSGliP35Gkb1ixqd1eisRmc1-kVNnnz693nnwV8XufD5xByC3z05vzt3BCjGJFP8D6SkmVE</recordid><startdate>20120721</startdate><enddate>20120721</enddate><creator>Shimoda, Mitsugi</creator><creator>Iwasaki, Yoshimi</creator><creator>Okada, Toshie</creator><creator>Kubota, Keiichi</creator><general>Baishideng Publishing Group Co., Limited</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120721</creationdate><title>Edaravone inhibits apoptosis caused by ischemia/ reperfusion injury in a porcine hepatectomy model</title><author>Shimoda, Mitsugi ; Iwasaki, Yoshimi ; Okada, Toshie ; Kubota, Keiichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-e1315b7ed37c3133d2cf64cb5cf4bce3653182ed39f4e94c262ca00f4751015e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antipyrine - analogs & derivatives</topic><topic>Antipyrine - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Biomarkers - blood</topic><topic>Cytoprotection</topic><topic>Disease Models, Animal</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Gene Expression Regulation</topic><topic>Hepatectomy - adverse effects</topic><topic>In Situ Nick-End Labeling</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Liver Regeneration - drug effects</topic><topic>Male</topic><topic>Original</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reperfusion Injury - etiology</topic><topic>Reperfusion Injury - genetics</topic><topic>Reperfusion Injury - metabolism</topic><topic>Reperfusion Injury - pathology</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Swine</topic><topic>Time Factors</topic><topic>Toll样受体</topic><topic>依达拉奉</topic><topic>切除术</topic><topic>模型</topic><topic>细胞凋亡</topic><topic>缺血/再灌注损伤</topic><topic>肝再生</topic><topic>逆转录聚合酶链反应</topic><toplevel>online_resources</toplevel><creatorcontrib>Shimoda, Mitsugi</creatorcontrib><creatorcontrib>Iwasaki, Yoshimi</creatorcontrib><creatorcontrib>Okada, Toshie</creatorcontrib><creatorcontrib>Kubota, Keiichi</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimoda, Mitsugi</au><au>Iwasaki, Yoshimi</au><au>Okada, Toshie</au><au>Kubota, Keiichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Edaravone inhibits apoptosis caused by ischemia/ reperfusion injury in a porcine hepatectomy model</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2012-07-21</date><risdate>2012</risdate><volume>18</volume><issue>27</issue><spage>3520</spage><epage>3526</epage><pages>3520-3526</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM： TO investigate the effect of E3-methyl-l-phe- nyl-2-pyrazolin-5-one （Edr） on hepatic ischemia-reper- fusion （I/R） injury and liver regeneration in a porcine hepatectomy model. METHODS： One hour ischemia was induced by occlud- ing the vessels and the bile duct of the right and median lobes. A 40% left hepatectomy was performed after re- perfusion. Six animals received Edr （3 mg/kg per hour） intravenously and six control animals received saline just before reperfusion. Remnant liver volume, hemody- namics, aspartate aminotransferase （AST）, alanine ami- notransferase, lactate dehydrogenase and lactic acid, were compared between the groups. The expression of transforming growth factor-β （TGF-β1） and toll-like receptor （TRL） mRNA in hepatic tissues was examined using reverse transcription polymerase chain reaction. Apoptosis was demonstrated by terminal deoxynucleo- tidyl transferase dUTP nick end labeling （TUNEL） stain- ing, respectively. RESULTS： Serum AS-I- （P = 0.029）, and toll like recep- tor 4 level （P = 0.043） were significantly lower after 3 hin animals receiving Edr. In addition, TUNEL staining in Edr-treated pigs showed significantly fewer hepatocytes undergoing apoptosis compared with control pigs. After 1 mo, all factors were non-significantly different between the two groups. CONCLUSION： Edr is considered to reduce hepatic injury in the early stage of I/R injury in a porcine model.</abstract><cop>United States</cop><pub>Baishideng Publishing Group Co., Limited</pub><pmid>22826616</pmid><doi>10.3748/wjg.v18.i27.3520</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Baishideng "World Journal of" online journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Antipyrine - analogs & derivatives Antipyrine - pharmacology Apoptosis - drug effects Biomarkers - blood Cytoprotection Disease Models, Animal Free Radical Scavengers - pharmacology Gene Expression Regulation Hepatectomy - adverse effects In Situ Nick-End Labeling Liver - drug effects Liver - metabolism Liver - pathology Liver Regeneration - drug effects Male Original Real-Time Polymerase Chain Reaction Reperfusion Injury - etiology Reperfusion Injury - genetics Reperfusion Injury - metabolism Reperfusion Injury - pathology Reperfusion Injury - prevention & control Reverse Transcriptase Polymerase Chain Reaction Swine Time Factors Toll样受体 依达拉奉 切除术 模型 细胞凋亡 缺血/再灌注损伤 肝再生 逆转录聚合酶链反应 |
| title | Edaravone inhibits apoptosis caused by ischemia/ reperfusion injury in a porcine hepatectomy model |
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