Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift
Shifting a yeast culture from an ethanol-based medium to a glucose-based medium causes a coordinate increase of the cellular levels of ribosomal protein mRNAs by about a factor 4 within 30 min. Making use of hybrid genes encompassing different portions of the 5'-flanking region of the L25-gene,...
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| Veröffentlicht in: | Nucleic acids research 1987-12, Vol.15 (24), p.10133-10144 |
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| container_issue | 24 |
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| container_title | Nucleic acids research |
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| creator | HERRUER, M. H MAGER, W. H WOUDT, L. P NIEUWINT, R. T. M WASSENAAR, G. M GROENEVELD, P PLANTA, R. J |
| description | Shifting a yeast culture from an ethanol-based medium to a glucose-based medium causes a coordinate increase of the cellular levels of ribosomal protein mRNAs by about a factor 4 within 30 min. Making use of hybrid genes encompassing different portions of the 5'-flanking region of the L25-gene, we could show that the increase in mRNAs is a transcriptional event, mediated through DNA sequences upstream of the ribosomal protein (rp) genes. Further analysis revealed that sequence elements are involved that many rp-genes have in common and that previously were identified as transcription activation sites (RPG-boxes or UASrpg). Using appropriate deletion mutants of the fusion genes we could demonstrate that a single RPG-box is sufficient for the transcriptional upshift. In addition, both copy genes encoding rp28 which differ considerably in their extent of transcriptional activity, show the upshift effect in a proportional manner. Definite proof for the role of the UASrpg in nutritional regulation was obtained by examining the effect of a synthetic RPG-box on transcription. |
| doi_str_mv | 10.1093/nar/15.24.10133 |
| format | Article |
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Using appropriate deletion mutants of the fusion genes we could demonstrate that a single RPG-box is sufficient for the transcriptional upshift. In addition, both copy genes encoding rp28 which differ considerably in their extent of transcriptional activity, show the upshift effect in a proportional manner. Definite proof for the role of the UASrpg in nutritional regulation was obtained by examining the effect of a synthetic RPG-box on transcription.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/15.24.10133</identifier><identifier>PMID: 3320961</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Ethanol - metabolism ; Fundamental and applied biological sciences. 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Further analysis revealed that sequence elements are involved that many rp-genes have in common and that previously were identified as transcription activation sites (RPG-boxes or UASrpg). Using appropriate deletion mutants of the fusion genes we could demonstrate that a single RPG-box is sufficient for the transcriptional upshift. In addition, both copy genes encoding rp28 which differ considerably in their extent of transcriptional activity, show the upshift effect in a proportional manner. Definite proof for the role of the UASrpg in nutritional regulation was obtained by examining the effect of a synthetic RPG-box on transcription.</description><subject>Biological and medical sciences</subject><subject>Ethanol - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - biosynthesis</subject><subject>Gene Expression Regulation</subject><subject>Glucose - metabolism</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Microbiology</subject><subject>Multigene Family</subject><subject>Mycology</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Ribosomal Proteins - biosynthesis</subject><subject>RNA, Fungal - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Transcription, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb9v1DAUxy1EVY7CzISUAXVLzy_PTpyBAVUUkCp1KQOT5Th2zyhnB78E6f57XHo6wdTJtr7fr9-PD2PvgF8B73EbTd6CvGpEeQLiC7YBbJta9G3zkm04clkDF-oVe030k3MQIMU5O0dseN_Chv24zyaSzWFeQopmqmyKS05TlXx1cIaWKochUdoXac5pcSFWdIjLzlGgalxziA-VNXlIsaa0ZuuqdaZd8MsbdubNRO7t8bxg328-319_rW_vvny7_nRbWynEUlvReal4M4JS0oMY0XthrUcnXbkNne2hGXpsnR2MU8rgYEawI7bQqs4ovGAfn_6d12HvRutK_2bScw57kw86maD_V2LY6Yf0WyP2PcqSvzzmc_q1Olr0PpB102SiSyvprlM98gafNUJZqOoKlGeNQpWJ4bH09slocyLKzp-6Bq4f8eqCV4PUjdB_8ZbE-3-HPfmPPIv-4agbsmbyBa4NdLJ1kred7PEPE8-xbw</recordid><startdate>19871223</startdate><enddate>19871223</enddate><creator>HERRUER, M. 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Molecular biology</topic><topic>Microbiology</topic><topic>Multigene Family</topic><topic>Mycology</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Ribosomal Proteins - biosynthesis</topic><topic>RNA, Fungal - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HERRUER, M. H</creatorcontrib><creatorcontrib>MAGER, W. H</creatorcontrib><creatorcontrib>WOUDT, L. P</creatorcontrib><creatorcontrib>NIEUWINT, R. T. M</creatorcontrib><creatorcontrib>WASSENAAR, G. M</creatorcontrib><creatorcontrib>GROENEVELD, P</creatorcontrib><creatorcontrib>PLANTA, R. 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| ispartof | Nucleic acids research, 1987-12, Vol.15 (24), p.10133-10144 |
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| language | eng |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central |
| subjects | Biological and medical sciences Ethanol - metabolism Fundamental and applied biological sciences. Psychology Fungal Proteins - biosynthesis Gene Expression Regulation Glucose - metabolism Growth, nutrition, metabolism, transports, enzymes. Molecular biology Microbiology Multigene Family Mycology Recombinant Fusion Proteins - genetics Regulatory Sequences, Nucleic Acid Ribosomal Proteins - biosynthesis RNA, Fungal - genetics RNA, Messenger - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Transcription, Genetic |
| title | Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift |
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