Comparison of Next-Generation Sequencing Systems
With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented...
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| Veröffentlicht in: | BioMed research international 2012-01, Vol.2012 (2012), p.1-11 |
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| creator | Lu, Lihua Law, Maggie Liu, Lin Lin, Danni Pong, Ray He, Yimin Li, Yinhu Li, Siliang Hu, Ni |
| description | With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world’s biggest sequencing capacity, has multiple NGS systems including 137 HiSeq 2000, 27 SOLiD, one Ion Torrent PGM, one MiSeq, and one 454 sequencer. We have accumulated extensive experience in sample handling, sequencing, and bioinformatics analysis. In this paper, technologies of these systems are reviewed, and first-hand data from extensive experience is summarized and analyzed to discuss the advantages and specifics associated with each sequencing system. At last, applications of NGS are summarized. |
| doi_str_mv | 10.1155/2012/251364 |
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J.</contributor><creatorcontrib>Lu, Lihua ; Law, Maggie ; Liu, Lin ; Lin, Danni ; Pong, Ray ; He, Yimin ; Li, Yinhu ; Li, Siliang ; Hu, Ni ; Oefner, P. J.</creatorcontrib><description>With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world’s biggest sequencing capacity, has multiple NGS systems including 137 HiSeq 2000, 27 SOLiD, one Ion Torrent PGM, one MiSeq, and one 454 sequencer. We have accumulated extensive experience in sample handling, sequencing, and bioinformatics analysis. In this paper, technologies of these systems are reviewed, and first-hand data from extensive experience is summarized and analyzed to discuss the advantages and specifics associated with each sequencing system. At last, applications of NGS are summarized.</description><identifier>ISSN: 1110-7243</identifier><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 1110-7251</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2012/251364</identifier><identifier>PMID: 22829749</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Accuracy ; Base Sequence ; Biology ; Deoxyribonucleic acid ; DNA ; Genomes ; Genomics ; Humans ; Review ; Sequence Analysis, DNA - economics ; Sequence Analysis, DNA - instrumentation ; Sequence Analysis, DNA - methods ; Software ; Technological change</subject><ispartof>BioMed research international, 2012-01, Vol.2012 (2012), p.1-11</ispartof><rights>Copyright © 2012 Lin Liu et al.</rights><rights>Copyright © 2012 Lin Liu et al. Lin Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2012 Lin Liu et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-54eadb44c8bbbad22787f8524566035dc80f9f8633df495b76d19f6fb66923e33</citedby><cites>FETCH-LOGICAL-c499t-54eadb44c8bbbad22787f8524566035dc80f9f8633df495b76d19f6fb66923e33</cites><orcidid>0000-0003-4293-5499 ; 0000-0002-5648-322X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398667/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398667/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22829749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Oefner, P. J.</contributor><creatorcontrib>Lu, Lihua</creatorcontrib><creatorcontrib>Law, Maggie</creatorcontrib><creatorcontrib>Liu, Lin</creatorcontrib><creatorcontrib>Lin, Danni</creatorcontrib><creatorcontrib>Pong, Ray</creatorcontrib><creatorcontrib>He, Yimin</creatorcontrib><creatorcontrib>Li, Yinhu</creatorcontrib><creatorcontrib>Li, Siliang</creatorcontrib><creatorcontrib>Hu, Ni</creatorcontrib><title>Comparison of Next-Generation Sequencing Systems</title><title>BioMed research international</title><addtitle>J Biomed Biotechnol</addtitle><description>With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world’s biggest sequencing capacity, has multiple NGS systems including 137 HiSeq 2000, 27 SOLiD, one Ion Torrent PGM, one MiSeq, and one 454 sequencer. We have accumulated extensive experience in sample handling, sequencing, and bioinformatics analysis. In this paper, technologies of these systems are reviewed, and first-hand data from extensive experience is summarized and analyzed to discuss the advantages and specifics associated with each sequencing system. 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J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Next-Generation Sequencing Systems</atitle><jtitle>BioMed research international</jtitle><addtitle>J Biomed Biotechnol</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>2012</volume><issue>2012</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>1110-7243</issn><issn>2314-6133</issn><eissn>1110-7251</eissn><eissn>2314-6141</eissn><abstract>With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. 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| source | MEDLINE; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Accuracy Base Sequence Biology Deoxyribonucleic acid DNA Genomes Genomics Humans Review Sequence Analysis, DNA - economics Sequence Analysis, DNA - instrumentation Sequence Analysis, DNA - methods Software Technological change |
| title | Comparison of Next-Generation Sequencing Systems |
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