The low ionic strength form of the sodium salt of poly(dm5C-dG) is a B DNA

The low ionic strength form of the sodium salt of poly(dm5C-dG) is a B DNA

The low-salt Z form of poly(dm5C-dG) reported in the literature was shown to be due to minute amounts of bivalent cations. If the polymer was purified by dialysis against 1 mM EDTA, poly(dm5C-dG) showed the normal B form. No change in this B form was observed up to 0.8 M NaCl. Very small amounts of...

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Veröffentlicht in:Nucleic acids research 1986-04, Vol.14 (8), p.3515-3519
Hauptverfasser: Woisard, Anne, Guschlbauer, Wilhelm, Fazakerley, G. Victor
Format: Artikel
Sprache:eng
Schlagworte:
Circular Dichroism
DNA
Kinetics
Nucleic Acid Conformation
Osmolar Concentration
Polydeoxyribonucleotides
Sodium
Spectrophotometry, Ultraviolet
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creator Woisard, Anne
Guschlbauer, Wilhelm
Fazakerley, G. Victor
description The low-salt Z form of poly(dm5C-dG) reported in the literature was shown to be due to minute amounts of bivalent cations. If the polymer was purified by dialysis against 1 mM EDTA, poly(dm5C-dG) showed the normal B form. No change in this B form was observed up to 0.8 M NaCl. Very small amounts of Mg2+ or Ca2+ (10−6 to 10−5M) induced the characteristic Z form CD spectrum, which could easily and rapidly be reverted to B-DNA by addition of EDTA.
doi_str_mv 10.1093/nar/14.8.3515
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source MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central
subjects Circular Dichroism
DNA
Kinetics
Nucleic Acid Conformation
Osmolar Concentration
Polydeoxyribonucleotides
Sodium
Spectrophotometry, Ultraviolet
title The low ionic strength form of the sodium salt of poly(dm5C-dG) is a B DNA
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