Dissection of promoter sequences involved in transcriptional activation of the Escherichia coli replication origin
The replication frequency of oriC plasmids in vivo is positively affected by specific transcripts running into oriC. These transcripts that activate oriC are initiated at a promoter of a gene coding for a 16kD protein. Genetic evidence is presented for binding of the initiation factor dnaA to a spec...
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| Veröffentlicht in: | Nucleic acids research 1986-03, Vol.14 (5), p.2333-2344 |
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| container_title | Nucleic acids research |
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| creator | Stuitje, A.R. Wind, N.de Spek, J.C.van der Pors, T.H. Meijer, M. |
| description | The replication frequency of oriC plasmids in vivo is positively affected by specific transcripts running into oriC. These transcripts that activate oriC are initiated at a promoter of a gene coding for a 16kD protein. Genetic evidence is presented for binding of the initiation factor dnaA to a specific sequence (dnaA box) upstream of this promoter. Binding of the dnaA protein to this dnaA box regulates transcription initiation negatively. It was also demonstrated that binding of dnaA protein to the 16kD promoter region is essential to accomplish the actual activation event within the origin. Replication and incompatibility experiments suggest that dnaA protein is present within the activating transcription complex. The function of dnaA in this replication control mechanism is discussed. |
| doi_str_mv | 10.1093/nar/14.5.2333 |
| format | Article |
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These transcripts that activate oriC are initiated at a promoter of a gene coding for a 16kD protein. Genetic evidence is presented for binding of the initiation factor dnaA to a specific sequence (dnaA box) upstream of this promoter. Binding of the dnaA protein to this dnaA box regulates transcription initiation negatively. It was also demonstrated that binding of dnaA protein to the 16kD promoter region is essential to accomplish the actual activation event within the origin. Replication and incompatibility experiments suggest that dnaA protein is present within the activating transcription complex. The function of dnaA in this replication control mechanism is discussed.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/14.5.2333</identifier><identifier>PMID: 2421247</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Bacterial Proteins - genetics ; Biological and medical sciences ; Chromosome Deletion ; DNA Replication ; DNA, Bacterial - genetics ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. 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These transcripts that activate oriC are initiated at a promoter of a gene coding for a 16kD protein. Genetic evidence is presented for binding of the initiation factor dnaA to a specific sequence (dnaA box) upstream of this promoter. Binding of the dnaA protein to this dnaA box regulates transcription initiation negatively. It was also demonstrated that binding of dnaA protein to the 16kD promoter region is essential to accomplish the actual activation event within the origin. Replication and incompatibility experiments suggest that dnaA protein is present within the activating transcription complex. The function of dnaA in this replication control mechanism is discussed.</description><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Chromosome Deletion</subject><subject>DNA Replication</subject><subject>DNA, Bacterial - genetics</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Genes, Bacterial</subject><subject>Genetic Engineering</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>Replication</subject><subject>Repressor Proteins - genetics</subject><subject>RNA, Bacterial - genetics</subject><subject>Transcription, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS0EKmngyBHJB8RtU39_HDigUigQCSEVqeJiOc5sY9ist_Ymov89jroEOPXkkd7vjWfmIfSCkgUllp_1Pp9RsZALxjl_hGaUK9YIq9hjNCOcyIYSYZ6i01J-EEIFleIEnTDBKBN6hvK7WAqEMaYepxYPOW3TCBkXuN1BH6Dg2O9Tt4d1LfCYfV9CjsOB9x321bj3f8zjBvBFCRvIMWyixyF1EWcYuhgmJseb2D9DT1rfFXg-vXP07f3F1flls_zy4eP522UThKZjA8rYWoWVCZa1liqtVpZQ4NZQZVgb7BrWRNZtueRt0ARaYkFL77kxujV8jt7c9x12qy2sA_R1_M4NOW59vnPJR_e_0seNu0l7x7lVilb_68mfUz1GGd02lgBd53tIu-K00loaIR8EqeWKCq0fBoU0hlZ8jpp7MORUSob2ODUl7pC6q6lX3El3SL3yL_9d9UhPMVf91aT7EnzX1hhDLEdMW22V5n-_jWWEX0fZ55-uylq6y-vv7qv49Hl5dc2c4L8B2LDHpQ</recordid><startdate>19860311</startdate><enddate>19860311</enddate><creator>Stuitje, A.R.</creator><creator>Wind, N.de</creator><creator>Spek, J.C.van der</creator><creator>Pors, T.H.</creator><creator>Meijer, M.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19860311</creationdate><title>Dissection of promoter sequences involved in transcriptional activation of the Escherichia coli replication origin</title><author>Stuitje, A.R. ; Wind, N.de ; Spek, J.C.van der ; Pors, T.H. ; Meijer, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-e689c47cb8c92f91676b901e3981682fc9ded05362353fc70ef09e75aa3887f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Chromosome Deletion</topic><topic>DNA Replication</topic><topic>DNA, Bacterial - genetics</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Genes, Bacterial</topic><topic>Genetic Engineering</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>Replication</topic><topic>Repressor Proteins - genetics</topic><topic>RNA, Bacterial - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stuitje, A.R.</creatorcontrib><creatorcontrib>Wind, N.de</creatorcontrib><creatorcontrib>Spek, J.C.van der</creatorcontrib><creatorcontrib>Pors, T.H.</creatorcontrib><creatorcontrib>Meijer, M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stuitje, A.R.</au><au>Wind, N.de</au><au>Spek, J.C.van der</au><au>Pors, T.H.</au><au>Meijer, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dissection of promoter sequences involved in transcriptional activation of the Escherichia coli replication origin</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1986-03-11</date><risdate>1986</risdate><volume>14</volume><issue>5</issue><spage>2333</spage><epage>2344</epage><pages>2333-2344</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>The replication frequency of oriC plasmids in vivo is positively affected by specific transcripts running into oriC. These transcripts that activate oriC are initiated at a promoter of a gene coding for a 16kD protein. Genetic evidence is presented for binding of the initiation factor dnaA to a specific sequence (dnaA box) upstream of this promoter. Binding of the dnaA protein to this dnaA box regulates transcription initiation negatively. It was also demonstrated that binding of dnaA protein to the 16kD promoter region is essential to accomplish the actual activation event within the origin. Replication and incompatibility experiments suggest that dnaA protein is present within the activating transcription complex. The function of dnaA in this replication control mechanism is discussed.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2421247</pmid><doi>10.1093/nar/14.5.2333</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1986-03, Vol.14 (5), p.2333-2344 |
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| language | eng |
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| source | MEDLINE; NCBI_PubMed Central（免费）; Oxford Journals Archive |
| subjects | Bacterial Proteins - genetics Biological and medical sciences Chromosome Deletion DNA Replication DNA, Bacterial - genetics Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes, Bacterial Genetic Engineering Molecular and cellular biology Molecular genetics Mutation Promoter Regions, Genetic Replication Repressor Proteins - genetics RNA, Bacterial - genetics Transcription, Genetic |
| title | Dissection of promoter sequences involved in transcriptional activation of the Escherichia coli replication origin |
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