Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene
we have investigated regulatory DNA elements in the expression of the drug metabolizing P-450c gene of rats. Aftar combining the 5′ flanking and upstream untranslated regions of the isolated P-450c gene with structural gene for chloramphenicol acetyltransferase (CAT), the fusion genes were transfect...
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| Veröffentlicht in: | Nucleic acids research 1986-02, Vol.14 (3), p.1465-1477 |
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| container_title | Nucleic acids research |
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| creator | ujisawa-Sehara, Atsuko ogawa, Kazuhiro Nishi, Chikako Fujii-Kuriyama, Yoshiaki |
| description | we have investigated regulatory DNA elements in the expression of the drug metabolizing P-450c gene of rats. Aftar combining the 5′ flanking and upstream untranslated regions of the isolated P-450c gene with structural gene for chloramphenicol acetyltransferase (CAT), the fusion genes were transfected into cultured cells (Kepa-1 and L cells) for the assay of transient expression of CAT activity. CAT activity was expressed inducibly in response to 3-methylcholanthrene only in Kepa-1 cells. At least three regions containing regulatory DNA elements were indentified; one, which is present in the sequence from −44b to −0.2kb immediately upstream of the TATA box, functions in the basal level of transcription, and the other two which were located in the sequence from −0.8kb to −1.0kb and from −1.0kb to −6.3kb, enhance in combination, transcription in response to inducers in a manner independent of their orientation. |
| doi_str_mv | 10.1093/nar/14.3.1465 |
| format | Article |
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Aftar combining the 5′ flanking and upstream untranslated regions of the isolated P-450c gene with structural gene for chloramphenicol acetyltransferase (CAT), the fusion genes were transfected into cultured cells (Kepa-1 and L cells) for the assay of transient expression of CAT activity. CAT activity was expressed inducibly in response to 3-methylcholanthrene only in Kepa-1 cells. At least three regions containing regulatory DNA elements were indentified; one, which is present in the sequence from −44b to −0.2kb immediately upstream of the TATA box, functions in the basal level of transcription, and the other two which were located in the sequence from −0.8kb to −1.0kb and from −1.0kb to −6.3kb, enhance in combination, transcription in response to inducers in a manner independent of their orientation.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/14.3.1465</identifier><identifier>PMID: 3456557</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Acetyltransferases - genetics ; Animals ; Biological and medical sciences ; Cell Line ; Chloramphenicol O-Acetyltransferase ; Chromosome Deletion ; Chromosome Mapping ; Cytochrome P-450 Enzyme System - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Genes, Regulator ; Genes. Genome ; Genetic Engineering ; Genetic Linkage ; L Cells (Cell Line) ; Liver Neoplasms, Experimental ; Methylcholanthrene - pharmacology ; Mice ; Molecular and cellular biology ; Molecular genetics ; Rats ; Transfection</subject><ispartof>Nucleic acids research, 1986-02, Vol.14 (3), p.1465-1477</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-2b8185d7e1fe0df2409ffa8d5d22333504e08a32e816a733a47704e0f254f8ef3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC339517/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC339517/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7966799$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3456557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ujisawa-Sehara, Atsuko</creatorcontrib><creatorcontrib>ogawa, Kazuhiro</creatorcontrib><creatorcontrib>Nishi, Chikako</creatorcontrib><creatorcontrib>Fujii-Kuriyama, Yoshiaki</creatorcontrib><title>Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>we have investigated regulatory DNA elements in the expression of the drug metabolizing P-450c gene of rats. Aftar combining the 5′ flanking and upstream untranslated regions of the isolated P-450c gene with structural gene for chloramphenicol acetyltransferase (CAT), the fusion genes were transfected into cultured cells (Kepa-1 and L cells) for the assay of transient expression of CAT activity. CAT activity was expressed inducibly in response to 3-methylcholanthrene only in Kepa-1 cells. At least three regions containing regulatory DNA elements were indentified; one, which is present in the sequence from −44b to −0.2kb immediately upstream of the TATA box, functions in the basal level of transcription, and the other two which were located in the sequence from −0.8kb to −1.0kb and from −1.0kb to −6.3kb, enhance in combination, transcription in response to inducers in a manner independent of their orientation.</description><subject>Acetyltransferases - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chloramphenicol O-Acetyltransferase</subject><subject>Chromosome Deletion</subject><subject>Chromosome Mapping</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes, Regulator</subject><subject>Genes. Genome</subject><subject>Genetic Engineering</subject><subject>Genetic Linkage</subject><subject>L Cells (Cell Line)</subject><subject>Liver Neoplasms, Experimental</subject><subject>Methylcholanthrene - pharmacology</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Rats</subject><subject>Transfection</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKkNhyRLJC8QuUz_jZMGiaoEiladAjNhYHuc6E3DiwXYQw6_Ho44GWHV1JZ_vPo4PQo8pWVLS8rPJxDMqlnxJRS3voAXlNatEW7O7aEE4kRUlormPHqT0jRAqqBQn6IQLWUupFgg-Qj97k0Pc4cu35xg8jDDlhH2wxg-_ocMRxpDB7_C8TTmCGbGLYcR5A7iLc1-NkM06FHaYemx3OdhN0QG_r4QkFvcwwUN0zxmf4NGhnqLPL198uriqrt-9en1xfl1ZSUmu2LqhjewUUAekc0yQ1jnTdLJjjHMuiQDSGM6gobVRnBuh1P7NMSlcA46fouc3c7fzeoTOFifReL2Nw2jiTgcz6P-VadjoPvzUnLeSqtL_7NAfw48ZUtbjkCx4byYIc9KqLluJIreCtPw_4XV7OygaSjgTBaxuQBtDShHc8WpK9D5oXYIuuOZ6H3Thn_xr9Ugfki3604NuUknSRTPZIR0x1da1atu_a4eU4ddRNvG7Ll6V1Ferr_rLB0pXK6n0G_4HhwPBQA</recordid><startdate>19860211</startdate><enddate>19860211</enddate><creator>ujisawa-Sehara, Atsuko</creator><creator>ogawa, Kazuhiro</creator><creator>Nishi, Chikako</creator><creator>Fujii-Kuriyama, Yoshiaki</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19860211</creationdate><title>Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene</title><author>ujisawa-Sehara, Atsuko ; ogawa, Kazuhiro ; Nishi, Chikako ; Fujii-Kuriyama, Yoshiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-2b8185d7e1fe0df2409ffa8d5d22333504e08a32e816a733a47704e0f254f8ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Acetyltransferases - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Chloramphenicol O-Acetyltransferase</topic><topic>Chromosome Deletion</topic><topic>Chromosome Mapping</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genes, Regulator</topic><topic>Genes. Genome</topic><topic>Genetic Engineering</topic><topic>Genetic Linkage</topic><topic>L Cells (Cell Line)</topic><topic>Liver Neoplasms, Experimental</topic><topic>Methylcholanthrene - pharmacology</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Rats</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ujisawa-Sehara, Atsuko</creatorcontrib><creatorcontrib>ogawa, Kazuhiro</creatorcontrib><creatorcontrib>Nishi, Chikako</creatorcontrib><creatorcontrib>Fujii-Kuriyama, Yoshiaki</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ujisawa-Sehara, Atsuko</au><au>ogawa, Kazuhiro</au><au>Nishi, Chikako</au><au>Fujii-Kuriyama, Yoshiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1986-02-11</date><risdate>1986</risdate><volume>14</volume><issue>3</issue><spage>1465</spage><epage>1477</epage><pages>1465-1477</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>we have investigated regulatory DNA elements in the expression of the drug metabolizing P-450c gene of rats. Aftar combining the 5′ flanking and upstream untranslated regions of the isolated P-450c gene with structural gene for chloramphenicol acetyltransferase (CAT), the fusion genes were transfected into cultured cells (Kepa-1 and L cells) for the assay of transient expression of CAT activity. CAT activity was expressed inducibly in response to 3-methylcholanthrene only in Kepa-1 cells. At least three regions containing regulatory DNA elements were indentified; one, which is present in the sequence from −44b to −0.2kb immediately upstream of the TATA box, functions in the basal level of transcription, and the other two which were located in the sequence from −0.8kb to −1.0kb and from −1.0kb to −6.3kb, enhance in combination, transcription in response to inducers in a manner independent of their orientation.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3456557</pmid><doi>10.1093/nar/14.3.1465</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1986-02, Vol.14 (3), p.1465-1477 |
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| subjects | Acetyltransferases - genetics Animals Biological and medical sciences Cell Line Chloramphenicol O-Acetyltransferase Chromosome Deletion Chromosome Mapping Cytochrome P-450 Enzyme System - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Genes, Regulator Genes. Genome Genetic Engineering Genetic Linkage L Cells (Cell Line) Liver Neoplasms, Experimental Methylcholanthrene - pharmacology Mice Molecular and cellular biology Molecular genetics Rats Transfection |
| title | Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene |
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