ADP-ribosylation is involved in the integration of foreign DNA into the mammalian cell genome
The most commonly used DNA transfection method, which employs the calcium phosphate co-precipitation of the donor DNA, involves several discrete steps (1,2). These include the uptake of the donor DNA by the recipient cells, the transport of the DNA to the nucleus, transient expression prior to integ...
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| Veröffentlicht in: | Nucleic acids research 1988-12, Vol.16 (23), p.11319-11326 |
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| container_title | Nucleic acids research |
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| creator | FARZIN FARZANEH PANAYOTOU, G. N BOWLER, L. D HARDAS, B. D BROOM, T WALTHER, C SHALL, S |
| description | The most commonly used DNA transfection method, which employs the calcium phosphate co-precipitation of the donor DNA, involves several discrete steps (1,2). These include the uptake of the donor DNA by the recipient cells, the transport of the DNA to the nucleus, transient expression prior to integration into the host cell genome, concatenation and integration of the transfected DNA into the host cell genome and finally the stable expression of the integrated genes (2,3). Both the concatenation and the integration of the donor DNA into the host genome involve the formation and ligation of DNA strand-breaks. In the present study we demonstrate that the nuclear enzyme, adenosine diphosphoribosyl transferase (ADPRT, E.C. 2.4.2.30), which is dependent on the presence of DNA strand breaks for its activity (4,5) and necessary for the efficient ligation of DNA strand-breaks in eukaryotic cells (4,6), is required for the integration of donor DNA into the host genome. However, ADPRT activity does not influence the uptake of DNA into the cell, its episomal maintenance or replication, nor its expression either before or after integration into the host genome. These observations strongly suggest the involvement of ADPRT activity in eukaryotic DNA recombination events. |
| doi_str_mv | 10.1093/nar/16.23.11319 |
| format | Article |
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N ; BOWLER, L. D ; HARDAS, B. D ; BROOM, T ; WALTHER, C ; SHALL, S</creator><creatorcontrib>FARZIN FARZANEH ; PANAYOTOU, G. N ; BOWLER, L. D ; HARDAS, B. D ; BROOM, T ; WALTHER, C ; SHALL, S</creatorcontrib><description>The most commonly used DNA transfection method, which employs the calcium phosphate co-precipitation of the donor DNA, involves several discrete steps (1,2). These include the uptake of the donor DNA by the recipient cells, the transport of the DNA to the nucleus, transient expression prior to integration into the host cell genome, concatenation and integration of the transfected DNA into the host cell genome and finally the stable expression of the integrated genes (2,3). Both the concatenation and the integration of the donor DNA into the host genome involve the formation and ligation of DNA strand-breaks. In the present study we demonstrate that the nuclear enzyme, adenosine diphosphoribosyl transferase (ADPRT, E.C. 2.4.2.30), which is dependent on the presence of DNA strand breaks for its activity (4,5) and necessary for the efficient ligation of DNA strand-breaks in eukaryotic cells (4,6), is required for the integration of donor DNA into the host genome. However, ADPRT activity does not influence the uptake of DNA into the cell, its episomal maintenance or replication, nor its expression either before or after integration into the host genome. These observations strongly suggest the involvement of ADPRT activity in eukaryotic DNA recombination events.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/16.23.11319</identifier><identifier>PMID: 3144706</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Benzamides ; Biological and medical sciences ; Cell Line ; DNA - metabolism ; DNA Replication - drug effects ; Fibroblasts - enzymology ; Fibroblasts - metabolism ; Fundamental and applied biological sciences. Psychology ; Genic rearrangement. Recombination. Transposable element ; Humans ; Mice ; Molecular and cellular biology ; Molecular genetics ; Poly(ADP-ribose) Polymerase Inhibitors ; Poly(ADP-ribose) Polymerases - physiology ; Transfection - drug effects ; Transformation, Genetic - drug effects</subject><ispartof>Nucleic acids research, 1988-12, Vol.16 (23), p.11319-11326</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-efafbe1e68dfe8edb30b9cc2016268eb2687057978ec53b07ec373a509e27f8a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC339012/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC339012/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6751877$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3144706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FARZIN FARZANEH</creatorcontrib><creatorcontrib>PANAYOTOU, G. N</creatorcontrib><creatorcontrib>BOWLER, L. D</creatorcontrib><creatorcontrib>HARDAS, B. D</creatorcontrib><creatorcontrib>BROOM, T</creatorcontrib><creatorcontrib>WALTHER, C</creatorcontrib><creatorcontrib>SHALL, S</creatorcontrib><title>ADP-ribosylation is involved in the integration of foreign DNA into the mammalian cell genome</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The most commonly used DNA transfection method, which employs the calcium phosphate co-precipitation of the donor DNA, involves several discrete steps (1,2). These include the uptake of the donor DNA by the recipient cells, the transport of the DNA to the nucleus, transient expression prior to integration into the host cell genome, concatenation and integration of the transfected DNA into the host cell genome and finally the stable expression of the integrated genes (2,3). Both the concatenation and the integration of the donor DNA into the host genome involve the formation and ligation of DNA strand-breaks. In the present study we demonstrate that the nuclear enzyme, adenosine diphosphoribosyl transferase (ADPRT, E.C. 2.4.2.30), which is dependent on the presence of DNA strand breaks for its activity (4,5) and necessary for the efficient ligation of DNA strand-breaks in eukaryotic cells (4,6), is required for the integration of donor DNA into the host genome. However, ADPRT activity does not influence the uptake of DNA into the cell, its episomal maintenance or replication, nor its expression either before or after integration into the host genome. These observations strongly suggest the involvement of ADPRT activity in eukaryotic DNA recombination events.</description><subject>Animals</subject><subject>Benzamides</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>DNA - metabolism</subject><subject>DNA Replication - drug effects</subject><subject>Fibroblasts - enzymology</subject><subject>Fibroblasts - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>Humans</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Poly(ADP-ribose) Polymerase Inhibitors</subject><subject>Poly(ADP-ribose) Polymerases - physiology</subject><subject>Transfection - drug effects</subject><subject>Transformation, Genetic - drug effects</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS0EKqFw5oS0B8RtE8_a648Dh6jlS6qAAxyR5XXGqdGuXexNpP73OE0UwakXj6X3e6OZeYS8BroEqtkq2rwCsezYEoCBfkIWwETXci26p2RBGe1boFw9Jy9K-U0pcOj5BblgwLmkYkF-ra-_tzkMqdyPdg4pNqE0Ie7TuMdN_TTzLdYy4zYf5eQbnzKGbWyuv64PUnpgJjtNdgw2Ng7HsdliTBO-JM-8HQu-OtVL8vPjhx9Xn9ubb5--XK1vWselmlv01g8IKNTGo8LNwOignesoiE4oHOojaS-1VOh6NlCJjklme6qxk15ZdkneH_ve7YYJNw7jnO1o7nKYbL43yQbzvxLDrdmmvWFMU-iq_93Jn9OfHZbZTKEc9rAR064YqXqthOKPglDvzpkWj4M9SKoEVHB1BF1OpWT056mBmkPEpkZsQJiOmYeIq-PNv8ue-VOmVX970m1xdvTZRhfKGROyByUl-wsSCrB3</recordid><startdate>19881209</startdate><enddate>19881209</enddate><creator>FARZIN FARZANEH</creator><creator>PANAYOTOU, G. N</creator><creator>BOWLER, L. D</creator><creator>HARDAS, B. D</creator><creator>BROOM, T</creator><creator>WALTHER, C</creator><creator>SHALL, S</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19881209</creationdate><title>ADP-ribosylation is involved in the integration of foreign DNA into the mammalian cell genome</title><author>FARZIN FARZANEH ; PANAYOTOU, G. N ; BOWLER, L. D ; HARDAS, B. D ; BROOM, T ; WALTHER, C ; SHALL, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-efafbe1e68dfe8edb30b9cc2016268eb2687057978ec53b07ec373a509e27f8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Benzamides</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>DNA - metabolism</topic><topic>DNA Replication - drug effects</topic><topic>Fibroblasts - enzymology</topic><topic>Fibroblasts - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>Humans</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Poly(ADP-ribose) Polymerase Inhibitors</topic><topic>Poly(ADP-ribose) Polymerases - physiology</topic><topic>Transfection - drug effects</topic><topic>Transformation, Genetic - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FARZIN FARZANEH</creatorcontrib><creatorcontrib>PANAYOTOU, G. N</creatorcontrib><creatorcontrib>BOWLER, L. D</creatorcontrib><creatorcontrib>HARDAS, B. D</creatorcontrib><creatorcontrib>BROOM, T</creatorcontrib><creatorcontrib>WALTHER, C</creatorcontrib><creatorcontrib>SHALL, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FARZIN FARZANEH</au><au>PANAYOTOU, G. 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These include the uptake of the donor DNA by the recipient cells, the transport of the DNA to the nucleus, transient expression prior to integration into the host cell genome, concatenation and integration of the transfected DNA into the host cell genome and finally the stable expression of the integrated genes (2,3). Both the concatenation and the integration of the donor DNA into the host genome involve the formation and ligation of DNA strand-breaks. In the present study we demonstrate that the nuclear enzyme, adenosine diphosphoribosyl transferase (ADPRT, E.C. 2.4.2.30), which is dependent on the presence of DNA strand breaks for its activity (4,5) and necessary for the efficient ligation of DNA strand-breaks in eukaryotic cells (4,6), is required for the integration of donor DNA into the host genome. 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| ispartof | Nucleic acids research, 1988-12, Vol.16 (23), p.11319-11326 |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Animals Benzamides Biological and medical sciences Cell Line DNA - metabolism DNA Replication - drug effects Fibroblasts - enzymology Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Genic rearrangement. Recombination. Transposable element Humans Mice Molecular and cellular biology Molecular genetics Poly(ADP-ribose) Polymerase Inhibitors Poly(ADP-ribose) Polymerases - physiology Transfection - drug effects Transformation, Genetic - drug effects |
| title | ADP-ribosylation is involved in the integration of foreign DNA into the mammalian cell genome |
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