Probing the 3-D Structure, Dynamics, and Stability of Bacterial Collagenase Collagen Binding Domain (apo- versus holo-) by Limited Proteolysis MALDI-TOF MS

Pairing limited proteolysis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to probe clostridial collagenase collagen binding domain (CBD) reveals the solution dynamics and stability of the protein, as these factors are crucial to CBD effectiveness as...

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Veröffentlicht in:	J. Am. Soc. Mass. Spectrom 2012-03, Vol.23 (3), p.505-519
Hauptverfasser:	Sides, Cynthia R., Liyanage, Rohana, Lay, Jackson O., Philominathan, Sagaya Theresa Leena, Matsushita, Osamu, Sakon, Joshua
Format:	Artikel
Sprache:	eng
Schlagworte:	Accessibility Analytical Chemistry Apoenzymes - chemistry Apoenzymes - metabolism Bacteria Binding Binding Sites Bioinformatics Biotechnology Calcium Calcium - chemistry Chemistry Chemistry and Materials Science Cleavage Clostridium histolyticum - enzymology Cobalt Cobalt - chemistry Crystal structure Crystallography Crystallography, X-Ray Desorption Drug delivery systems Dynamic stability Enzyme Stability Ionization Ions Lasers Mass spectrometry Microbial Collagenase - chemistry Microbial Collagenase - metabolism Models, Molecular Organic Chemistry Peptide Fragments - chemistry Peptide Fragments - metabolism Phase stability Protein Conformation Proteins Proteomics Research Article Serine Endopeptidases - chemistry Serine Endopeptidases - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Stability analysis Structural stability Trypsin - chemistry Trypsin - metabolism X-ray scattering
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