Identification of Methylated Proteins in the Yeast Small Ribosomal Subunit: A Role for SPOUT Methyltransferases in Protein Arginine Methylation

We have characterized the posttranslational methylation of Rps2, Rps3, and Rps27a, three small ribosomal subunit proteins in the yeast Saccharomyces cerevisiae, using mass spectrometry and amino acid analysis. We found that Rps2 is substoichiometrically modified at arginine-10 by the Rmt1 methyltran...

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Veröffentlicht in:	Biochemistry (Easton) 2012-06, Vol.51 (25), p.5091-5104
Hauptverfasser:	Young, Brian D, Weiss, David I, Zurita-Lopez, Cecilia I, Webb, Kristofor J, Clarke, Steven G, McBride, Anne E
Format:	Artikel
Sprache:	eng
Schlagworte:	alanine amino acid composition arginine Arginine - genetics Arginine - metabolism biogenesis cell growth cysteine Cysteine - metabolism genes mass spectrometry Methylation methyltransferases Multigene Family - physiology Mutagenesis, Site-Directed mutagenicity mutants Protein Processing, Post-Translational - genetics protein subunits Protein-Arginine N-Methyltransferases - genetics Protein-Arginine N-Methyltransferases - metabolism ribosomal proteins Ribosomal Proteins - genetics Ribosomal Proteins - metabolism ribosomal RNA Ribosome Subunits, Small - genetics Ribosome Subunits, Small - metabolism ribosomes S-adenosylmethionine Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism screening yeasts Zinc - metabolism
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