Primary structure of the Leishmania donovani small subunit ribosomal RNA coding region
The small subunit ribosomal RNA coding region for the Sudan I strain of Leishmania donovani is contained within three EcoR1/EcoR1 restriction fragments 3.7 kb, 1.0 kb, and 56 bp in size. The 3.7 kb and 1 kb fragments were cloned into PTZ 18U plasmids and a 450 bp HindIII/Pst1 which contains the 56 b...
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| Veröffentlicht in: | Nucleic acids research 1988-07, Vol.16 (14B), p.7198-7198 |
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| container_issue | 14B |
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| container_title | Nucleic acids research |
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| creator | LOOKER, D MILLER, L. A ELWOOD, H. J STICKEL, S SOGIN, M. L |
| description | The small subunit ribosomal RNA coding region for the Sudan I strain of Leishmania donovani is contained within three EcoR1/EcoR1 restriction fragments 3.7 kb, 1.0 kb, and 56 bp in size. The 3.7 kb and 1 kb fragments were cloned into PTZ 18U plasmids and a 450 bp HindIII/Pst1 which contains the 56 bp fragment and overlaps the 3.7 kb and 1.0 kb EcoR1 fragments was directly cloned into M13 phage mp18. Complete sequences for both strands of the coding region were determined. |
| doi_str_mv | 10.1093/nar/16.14.7198 |
| format | Article |
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Complete sequences for both strands of the coding region were determined.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/16.14.7198</identifier><identifier>PMID: 3405771</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; Genes. 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A</creatorcontrib><creatorcontrib>ELWOOD, H. J</creatorcontrib><creatorcontrib>STICKEL, S</creatorcontrib><creatorcontrib>SOGIN, M. L</creatorcontrib><title>Primary structure of the Leishmania donovani small subunit ribosomal RNA coding region</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The small subunit ribosomal RNA coding region for the Sudan I strain of Leishmania donovani is contained within three EcoR1/EcoR1 restriction fragments 3.7 kb, 1.0 kb, and 56 bp in size. The 3.7 kb and 1 kb fragments were cloned into PTZ 18U plasmids and a 450 bp HindIII/Pst1 which contains the 56 bp fragment and overlaps the 3.7 kb and 1.0 kb EcoR1 fragments was directly cloned into M13 phage mp18. Complete sequences for both strands of the coding region were determined.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>RNA, Ribosomal - genetics</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxYMo67h69SbkIN56NtWVdDoHD8viFwwqol5DOl09E-lO1qR7wf_eXmYY9LSnKur9XlHFY-wliC0Ig1fR5StotiC3Gkz7iG0Am7qSpqkfs41AoSoQsn3KnpXySwiQoOQFu0AplNawYT-_5jC5_IeXOS9-XjLxNPD5QHxHoRwmF4PjfYrpbu14mdw48rJ0Swwzz6FLJa0j_u3zNfepD3HPM-1Dis_Zk8GNhV6c6iX78f7d95uP1e7Lh08317vKS1RzNQAQuNppRG8EUgNSNbJ3w6oaaqVsao1ODWIwuvem7Toi0rIT6MjIDvGSvT3uvV26iXpPcc5utLfHp2xywf6vxHCw-3RnEVvUZvW_Oflz-r1Qme0UiqdxdJHSUqxusZFGqQfBWgglWgkPgqAQQdVyBbdH0OdUSqbhfDUIex-tXaO10FiQ9j7a1fDq31_P-CnLVX990l3xbhyyiz6UM6Zr1NIA_gUzFK25</recordid><startdate>19880725</startdate><enddate>19880725</enddate><creator>LOOKER, D</creator><creator>MILLER, L. 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L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-f11e1a2a733c903e614564daf4359e8446273a5f0f97dc98bbeee74b03ae94b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>RNA, Ribosomal - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LOOKER, D</creatorcontrib><creatorcontrib>MILLER, L. A</creatorcontrib><creatorcontrib>ELWOOD, H. 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L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Primary structure of the Leishmania donovani small subunit ribosomal RNA coding region</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1988-07-25</date><risdate>1988</risdate><volume>16</volume><issue>14B</issue><spage>7198</spage><epage>7198</epage><pages>7198-7198</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>The small subunit ribosomal RNA coding region for the Sudan I strain of Leishmania donovani is contained within three EcoR1/EcoR1 restriction fragments 3.7 kb, 1.0 kb, and 56 bp in size. The 3.7 kb and 1 kb fragments were cloned into PTZ 18U plasmids and a 450 bp HindIII/Pst1 which contains the 56 bp fragment and overlaps the 3.7 kb and 1.0 kb EcoR1 fragments was directly cloned into M13 phage mp18. Complete sequences for both strands of the coding region were determined.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3405771</pmid><doi>10.1093/nar/16.14.7198</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1988-07, Vol.16 (14B), p.7198-7198 |
| issn | 0305-1048 1362-4962 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Animals Base Sequence Biological and medical sciences Cloning, Molecular DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Genes. Genome Leishmania donovani Leishmania donovani - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data RNA, Ribosomal - genetics |
| title | Primary structure of the Leishmania donovani small subunit ribosomal RNA coding region |
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