A mini-screen technique for analyzing nuclear DNA from a single Dictyostelium colony
The cellular Dictyostelium slime molds have been found to carry a number of different multi-copy, circular, nuclear plasmids. In order to facilitate the screening of wild isolates for endogenous plasmids, and of transformants for the presence of extrachromosomal or integrated vector sequences the au...
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| Veröffentlicht in: | Nucleic acids research 1988-03, Vol.16 (5), p.2338-2338 |
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| container_title | Nucleic acids research |
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| creator | HUGHES, J. E WELKER, D. L |
| description | The cellular Dictyostelium slime molds have been found to carry a number of different multi-copy, circular, nuclear plasmids. In order to facilitate the screening of wild isolates for endogenous plasmids, and of transformants for the presence of extrachromosomal or integrated vector sequences the authors have developed a rapid and reliable mini-screening technique which allows sufficient plasmid or total nuclear DNA to be obtained from a single 2 cm diameter colony of Dictyostelium , and eliminates many of the extraneous bands seen with lysis-in-the-gel procedures. |
| doi_str_mv | 10.1093/nar/16.5.2338 |
| format | Article |
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In order to facilitate the screening of wild isolates for endogenous plasmids, and of transformants for the presence of extrachromosomal or integrated vector sequences the authors have developed a rapid and reliable mini-screening technique which allows sufficient plasmid or total nuclear DNA to be obtained from a single 2 cm diameter colony of Dictyostelium , and eliminates many of the extraneous bands seen with lysis-in-the-gel procedures.</description><subject>Biological and medical sciences</subject><subject>Cell Nucleus - analysis</subject><subject>Dictyostelium</subject><subject>Dictyostelium - genetics</subject><subject>Dictyostelium discoideum</subject><subject>DNA - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. 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In order to facilitate the screening of wild isolates for endogenous plasmids, and of transformants for the presence of extrachromosomal or integrated vector sequences the authors have developed a rapid and reliable mini-screening technique which allows sufficient plasmid or total nuclear DNA to be obtained from a single 2 cm diameter colony of Dictyostelium , and eliminates many of the extraneous bands seen with lysis-in-the-gel procedures.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3357783</pmid><doi>10.1093/nar/16.5.2338</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1988-03, Vol.16 (5), p.2338-2338 |
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| language | eng |
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| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Biological and medical sciences Cell Nucleus - analysis Dictyostelium Dictyostelium - genetics Dictyostelium discoideum DNA - analysis Fundamental and applied biological sciences. Psychology Genes. Genome Microchemistry Molecular and cellular biology Molecular genetics |
| title | A mini-screen technique for analyzing nuclear DNA from a single Dictyostelium colony |
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