Structure and Mechanism of the CMR Complex for CRISPR-Mediated Antiviral Immunity

The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subs...

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Veröffentlicht in:Molecular cell 2012-02, Vol.45 (3), p.303-313
Hauptverfasser: Zhang, Jing, Rouillon, Christophe, Kerou, Melina, Reeks, Judith, Brugger, Kim, Graham, Shirley, Reimann, Julia, Cannone, Giuseppe, Liu, Huanting, Albers, Sonja-Verena, Naismith, James H., Spagnolo, Laura, White, Malcolm F.
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container_end_page 313
container_issue 3
container_start_page 303
container_title Molecular cell
container_volume 45
creator Zhang, Jing
Rouillon, Christophe
Kerou, Melina
Reeks, Judith
Brugger, Kim
Graham, Shirley
Reimann, Julia
Cannone, Giuseppe
Liu, Huanting
Albers, Sonja-Verena
Naismith, James H.
Spagnolo, Laura
White, Malcolm F.
description The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse “payload” of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endonucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5′ sequence in the crRNA, but not on the presence of a protospacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets. [Display omitted] ► EM structure of the CMR complex for viral RNA degradation has been determined ► The crRNA content of CMR has been analyzed by deep sequencing ► Target RNA cleavage by CMR is sequence dependent
doi_str_mv 10.1016/j.molcel.2011.12.013
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RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse “payload” of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endonucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5′ sequence in the crRNA, but not on the presence of a protospacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets. 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RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse “payload” of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endonucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5′ sequence in the crRNA, but not on the presence of a protospacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets. 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RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse “payload” of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endonucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5′ sequence in the crRNA, but not on the presence of a protospacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets. 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subjects Archaeal Proteins - chemistry
Archaeal Proteins - isolation & purification
Archaeal Viruses - immunology
Base Sequence
Cellular apoptosis susceptibility protein
Crystal structure
Crystallography, X-Ray
DNA
Immunity
Infection
Inverted Repeat Sequences
Macromolecular Substances - chemistry
Macromolecular Substances - isolation & purification
Microscopy, Electron
Models, Molecular
Molecular Sequence Data
Nucleic Acid Conformation
Protein Structure, Quaternary
Protein Structure, Tertiary
Protein Subunits - chemistry
Protein Subunits - isolation & purification
Ribonucleoproteins
RNA
RNA Cleavage
RNA, Archaeal - chemistry
RNA, Archaeal - genetics
RNA, Archaeal - isolation & purification
Sulfolobus solfataricus
Sulfolobus solfataricus - genetics
Sulfolobus solfataricus - immunology
Sulfolobus solfataricus - metabolism
Sulfolobus solfataricus - virology
title Structure and Mechanism of the CMR Complex for CRISPR-Mediated Antiviral Immunity
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