Expression and Characterization of Recombinant Ecarin

The snake venom protease ecarin from Echis carinatus was expressed in stable transfected CHO-S cells grown in animal component free cell culture medium. Recombinant ecarin (r-ecarin) was secreted from the suspension adapted Chinese Hamster Ovary (CHO-S) host cells as a pro-protein and activation to...

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Veröffentlicht in:	Protein Journal 2012-06, Vol.31 (5), p.353-358
Hauptverfasser:	Jonebring, Anna, Lange, Ute, Bucha, Elke, Deinum, Johanna, Elg, Margareta, Lövgren, Ann
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Anatomy Animals Biochemistry Bioorganic Chemistry Cell culture Chemistry Chemistry and Materials Science CHO Cells Cloning, Molecular Cricetinae Echis carinatus Endopeptidases - genetics Endopeptidases - metabolism Enzyme Activation Fibrinolytic Agents - metabolism Gene Expression Histology Humans Kinetics Morphology Organic Chemistry Proteases Proteinase Proteins Prothrombin Prothrombin - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Snakes Thrombin Venom Viper Venoms - genetics Viper Venoms - metabolism Viperidae - genetics Viperidae - metabolism
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