Development of Animal Model for Studying Deep Second-Degree Thermal Burns
Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation...
Gespeichert in:
| Veröffentlicht in: | BioMed research international 2012-01, Vol.2012 (2012), p.1-7 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7 |
|---|---|
| container_issue | 2012 |
| container_start_page | 1 |
| container_title | BioMed research international |
| container_volume | 2012 |
| creator | dos Santos Tavares Pereira, Danielle Madruga Lima-Ribeiro, Maria Helena de Pontes-Filho, Nicodemos Teles dos Anjos Carneiro-Leão, Ana Maria dos Santos Correia, Maria Tereza |
| description | Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation of a thick and dry crust from the 3rd day. The percentage of tissue shrinkage at 28 days was 66.67 ± 1.66%. There was no sign of infection, bleeding, or secretion. Within 28 days reepithelialization was incomplete, with fibroblastic proliferation and moderate fibrosis and presence of modeled dense collagen fibers. It is concluded that the model established is applicable in obtaining deep second-degree thermal burns in order to evaluate the healing action of therapeutic agents of topical use. |
| doi_str_mv | 10.1155/2012/460841 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3379528</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1022562954</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-e79f831325825490e237f4ceb270b2d17786b9d7d0939fe728a2e12c9c75a0a3</originalsourceid><addsrcrecordid>eNqF0U1P3DAQBmALUfHVnnouisQFUaV4xnYcXyoBSwGJqgf2bnmTyW5QNt7aGxD_Hq9CV20vnGzJj16N52XsM_BvAEqdIwc8lwUvJeywAwDguUYFu9u7FPvsMMZHzkGXhdlj-4haFEbBAbub0BN1frWkfp35Jrvo26Xrsp--pi5rfMge1kP90vbzbEK0yh6o8n2dT2geiLLpgsJGXw6hjx_Zh8Z1kT69nUds-uN6enWb3_-6ubu6uM8racw6J22aUoBAVaKShhMK3ciKZqj5DGvQacSZqXXNjTANaSwdEmBlKq0cd-KIfR9jV8NsSXWV5g6us6uQ5g4v1rvW_vvStws7909WCG0Uling9C0g-N8DxbVdtrGirnM9-SFaQASQSmn-PuWIqkCjZKIn_9FHn7aSFrFRWuii4JjU11FVwccYqNnODdxuyrSbMu1YZtLHf391a_-0l8DZCBZtX7vn9p20LyOmRKhxWyy1EJKLV2WRrds</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1027376602</pqid></control><display><type>article</type><title>Development of Animal Model for Studying Deep Second-Degree Thermal Burns</title><source>MEDLINE</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>dos Santos Tavares Pereira, Danielle ; Madruga Lima-Ribeiro, Maria Helena ; de Pontes-Filho, Nicodemos Teles ; dos Anjos Carneiro-Leão, Ana Maria ; dos Santos Correia, Maria Tereza</creator><contributor>Fedele, Monica</contributor><creatorcontrib>dos Santos Tavares Pereira, Danielle ; Madruga Lima-Ribeiro, Maria Helena ; de Pontes-Filho, Nicodemos Teles ; dos Anjos Carneiro-Leão, Ana Maria ; dos Santos Correia, Maria Tereza ; Fedele, Monica</creatorcontrib><description>Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation of a thick and dry crust from the 3rd day. The percentage of tissue shrinkage at 28 days was 66.67 ± 1.66%. There was no sign of infection, bleeding, or secretion. Within 28 days reepithelialization was incomplete, with fibroblastic proliferation and moderate fibrosis and presence of modeled dense collagen fibers. It is concluded that the model established is applicable in obtaining deep second-degree thermal burns in order to evaluate the healing action of therapeutic agents of topical use.</description><identifier>ISSN: 1110-7243</identifier><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 1110-7251</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2012/460841</identifier><identifier>PMID: 22736951</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Aluminum ; Animal skins ; Animals ; Burns ; Burns - pathology ; Burns - physiopathology ; Disease Models, Animal ; Edema ; Evolution ; Histocytochemistry ; Injuries ; Ketamine ; Male ; Methodology Report ; Rats ; Rats, Wistar ; Reproducibility of Results ; Skin - pathology ; Skin - physiopathology ; Wound healing ; Wound Healing - physiology</subject><ispartof>BioMed research international, 2012-01, Vol.2012 (2012), p.1-7</ispartof><rights>Copyright © 2012 Danielle dos Santos Tavares Pereira et al.</rights><rights>Copyright © 2012 Danielle dos Santos Tavares Pereira et al. Danielle dos Santos Tavares Pereira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2012 Danielle dos Santos Tavares Pereira et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-e79f831325825490e237f4ceb270b2d17786b9d7d0939fe728a2e12c9c75a0a3</citedby><cites>FETCH-LOGICAL-c499t-e79f831325825490e237f4ceb270b2d17786b9d7d0939fe728a2e12c9c75a0a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3379528/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3379528/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22736951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Fedele, Monica</contributor><creatorcontrib>dos Santos Tavares Pereira, Danielle</creatorcontrib><creatorcontrib>Madruga Lima-Ribeiro, Maria Helena</creatorcontrib><creatorcontrib>de Pontes-Filho, Nicodemos Teles</creatorcontrib><creatorcontrib>dos Anjos Carneiro-Leão, Ana Maria</creatorcontrib><creatorcontrib>dos Santos Correia, Maria Tereza</creatorcontrib><title>Development of Animal Model for Studying Deep Second-Degree Thermal Burns</title><title>BioMed research international</title><addtitle>J Biomed Biotechnol</addtitle><description>Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation of a thick and dry crust from the 3rd day. The percentage of tissue shrinkage at 28 days was 66.67 ± 1.66%. There was no sign of infection, bleeding, or secretion. Within 28 days reepithelialization was incomplete, with fibroblastic proliferation and moderate fibrosis and presence of modeled dense collagen fibers. It is concluded that the model established is applicable in obtaining deep second-degree thermal burns in order to evaluate the healing action of therapeutic agents of topical use.</description><subject>Aluminum</subject><subject>Animal skins</subject><subject>Animals</subject><subject>Burns</subject><subject>Burns - pathology</subject><subject>Burns - physiopathology</subject><subject>Disease Models, Animal</subject><subject>Edema</subject><subject>Evolution</subject><subject>Histocytochemistry</subject><subject>Injuries</subject><subject>Ketamine</subject><subject>Male</subject><subject>Methodology Report</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reproducibility of Results</subject><subject>Skin - pathology</subject><subject>Skin - physiopathology</subject><subject>Wound healing</subject><subject>Wound Healing - physiology</subject><issn>1110-7243</issn><issn>2314-6133</issn><issn>1110-7251</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0U1P3DAQBmALUfHVnnouisQFUaV4xnYcXyoBSwGJqgf2bnmTyW5QNt7aGxD_Hq9CV20vnGzJj16N52XsM_BvAEqdIwc8lwUvJeywAwDguUYFu9u7FPvsMMZHzkGXhdlj-4haFEbBAbub0BN1frWkfp35Jrvo26Xrsp--pi5rfMge1kP90vbzbEK0yh6o8n2dT2geiLLpgsJGXw6hjx_Zh8Z1kT69nUds-uN6enWb3_-6ubu6uM8racw6J22aUoBAVaKShhMK3ciKZqj5DGvQacSZqXXNjTANaSwdEmBlKq0cd-KIfR9jV8NsSXWV5g6us6uQ5g4v1rvW_vvStws7909WCG0Uling9C0g-N8DxbVdtrGirnM9-SFaQASQSmn-PuWIqkCjZKIn_9FHn7aSFrFRWuii4JjU11FVwccYqNnODdxuyrSbMu1YZtLHf391a_-0l8DZCBZtX7vn9p20LyOmRKhxWyy1EJKLV2WRrds</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>dos Santos Tavares Pereira, Danielle</creator><creator>Madruga Lima-Ribeiro, Maria Helena</creator><creator>de Pontes-Filho, Nicodemos Teles</creator><creator>dos Anjos Carneiro-Leão, Ana Maria</creator><creator>dos Santos Correia, Maria Tereza</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>Development of Animal Model for Studying Deep Second-Degree Thermal Burns</title><author>dos Santos Tavares Pereira, Danielle ; Madruga Lima-Ribeiro, Maria Helena ; de Pontes-Filho, Nicodemos Teles ; dos Anjos Carneiro-Leão, Ana Maria ; dos Santos Correia, Maria Tereza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-e79f831325825490e237f4ceb270b2d17786b9d7d0939fe728a2e12c9c75a0a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aluminum</topic><topic>Animal skins</topic><topic>Animals</topic><topic>Burns</topic><topic>Burns - pathology</topic><topic>Burns - physiopathology</topic><topic>Disease Models, Animal</topic><topic>Edema</topic><topic>Evolution</topic><topic>Histocytochemistry</topic><topic>Injuries</topic><topic>Ketamine</topic><topic>Male</topic><topic>Methodology Report</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reproducibility of Results</topic><topic>Skin - pathology</topic><topic>Skin - physiopathology</topic><topic>Wound healing</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>dos Santos Tavares Pereira, Danielle</creatorcontrib><creatorcontrib>Madruga Lima-Ribeiro, Maria Helena</creatorcontrib><creatorcontrib>de Pontes-Filho, Nicodemos Teles</creatorcontrib><creatorcontrib>dos Anjos Carneiro-Leão, Ana Maria</creatorcontrib><creatorcontrib>dos Santos Correia, Maria Tereza</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>dos Santos Tavares Pereira, Danielle</au><au>Madruga Lima-Ribeiro, Maria Helena</au><au>de Pontes-Filho, Nicodemos Teles</au><au>dos Anjos Carneiro-Leão, Ana Maria</au><au>dos Santos Correia, Maria Tereza</au><au>Fedele, Monica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Animal Model for Studying Deep Second-Degree Thermal Burns</atitle><jtitle>BioMed research international</jtitle><addtitle>J Biomed Biotechnol</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>2012</volume><issue>2012</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1110-7243</issn><issn>2314-6133</issn><eissn>1110-7251</eissn><eissn>2314-6141</eissn><abstract>Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation of a thick and dry crust from the 3rd day. The percentage of tissue shrinkage at 28 days was 66.67 ± 1.66%. There was no sign of infection, bleeding, or secretion. Within 28 days reepithelialization was incomplete, with fibroblastic proliferation and moderate fibrosis and presence of modeled dense collagen fibers. It is concluded that the model established is applicable in obtaining deep second-degree thermal burns in order to evaluate the healing action of therapeutic agents of topical use.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>22736951</pmid><doi>10.1155/2012/460841</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1110-7243 |
| ispartof | BioMed research international, 2012-01, Vol.2012 (2012), p.1-7 |
| issn | 1110-7243 2314-6133 1110-7251 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3379528 |
| source | MEDLINE; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Aluminum Animal skins Animals Burns Burns - pathology Burns - physiopathology Disease Models, Animal Edema Evolution Histocytochemistry Injuries Ketamine Male Methodology Report Rats Rats, Wistar Reproducibility of Results Skin - pathology Skin - physiopathology Wound healing Wound Healing - physiology |
| title | Development of Animal Model for Studying Deep Second-Degree Thermal Burns |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T23%3A52%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20Animal%20Model%20for%20Studying%20Deep%20Second-Degree%20Thermal%20Burns&rft.jtitle=BioMed%20research%20international&rft.au=dos%20Santos%20Tavares%20Pereira,%20Danielle&rft.date=2012-01-01&rft.volume=2012&rft.issue=2012&rft.spage=1&rft.epage=7&rft.pages=1-7&rft.issn=1110-7243&rft.eissn=1110-7251&rft_id=info:doi/10.1155/2012/460841&rft_dat=%3Cproquest_pubme%3E1022562954%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1027376602&rft_id=info:pmid/22736951&rfr_iscdi=true |