Substrate recognition mechanism of a glycosyltrehalose trehalohydrolase from Sulfolobus solfataricus KM1

Glycosyltrehalose trehalohydrolase (GTHase) is an α‐amylase that cleaves the α‐1,4 bond adjacent to the α‐1,1 bond of maltooligosyltrehalose to release trehalose. To investigate the catalytic and substrate recognition mechanisms of GTHase, two residues, Asp252 (nucleophile) and Glu283 (general acid/...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Protein science 2012-04, Vol.21 (4), p.539-552
Hauptverfasser:	Okazaki, Nobuo, Tamada, Taro, Feese, Michael D., Kato, Masaru, Miura, Yutaka, Komeda, Toshihiro, Kobayashi, Kazuo, Kondo, Keiji, Blaber, Michael, Kuroki, Ryota
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - chemistry Amino Acid Substitution Amino Acids - chemistry Bacterial Proteins - chemistry Catalytic Domain Crystallography, X-Ray Enzyme Activation Enzyme Assays Hydrogen Bonding maltotriosyltrehalose Models, Molecular Multiprotein Complexes - chemistry Protein Binding Protein Interaction Mapping Structure-Activity Relationship substrate recognition Substrate Specificity Sulfolobus solfataricus - chemistry Sulfolobus solfataricus - enzymology Sulfolobus solfataricus - genetics trehalohydrolase trehalose production α‐amylase
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!