Isolation and characterisation of restriction endonuclease BcuAI from Bacillus cereus A

Restriction endonuclease BcuAI, an isoschizomer of AvaII, has been purified from Bacillus cereus A. The procedure of isolation of BcuAI included the fractionation with ammonium sulfate and column chromatography on DEAE-Sepharose (elution buffer 10 mM K-phosphate, pH 7.4, 0.1 mM EDTA, 1 mM DTT, 0.0-1...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Nucleic acids research 1992-06, Vol.20 (11), p.2898-2898
Hauptverfasser:	ELDAROV, M. A, KARPICHEV, I. V, SAMKO, O. T, ANIKEITCHEVA, N. V, KALUGIN, A. A, KHOROSHOUTINA, E. B, SOKOLOV, N. N
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Bacillus cereus Bacillus cereus - enzymology Biological and medical sciences Deoxyribonucleases, Type II Site-Specific - isolation & purification Deoxyribonucleases, Type II Site-Specific - metabolism Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Substrate Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!