Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis
We have determined the entire sequence of the plasmid K2 from Kluyveromyces lactis which is involved in the maintenance of both killer plasmids in the cell. K2 shares many of the characteristics of the smaller killer plasmid K1: high A+T content (74.7%) and very compact genomic organization. K2 cont...
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| Veröffentlicht in: | Nucleic acids research 1988-07, Vol.16 (13), p.5863-5878 |
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| creator | TOMMASINO, M RICCI, S GALEOTTI, C. L |
| description | We have determined the entire sequence of the plasmid K2 from Kluyveromyces lactis which is involved in the maintenance of both killer plasmids in the cell. K2 shares many of the characteristics of the smaller killer plasmid K1: high A+T content (74.7%) and very compact genomic organization. K2 contains ten open reading frames. Some of them overlap on different strands and some on the same strand. Northern blotting of K2 transcripts shows that at least eight ORFs are transcribed. Analysis of the predicted aminoacid sequence of ORF2 from K2 reveals homology with the aminoacid sequence of ORF 1 from K1 and with several viral DNA polymerases. The sequence of K2 from Saccaromyces cerevisiae F102-2 was also determined. Only one nucleotide difference was found between the K2 sequence from the two yeasts. This mutation does not change the genome organization of the plasmid and has only minimal effect on the structure of the encoded proteins. |
| doi_str_mv | 10.1093/nar/16.13.5863 |
| format | Article |
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This mutation does not change the genome organization of the plasmid and has only minimal effect on the structure of the encoded proteins.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/16.13.5863</identifier><identifier>PMID: 3041369</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; DNA, Fungal - analysis ; Fundamental and applied biological sciences. Psychology ; Genes, Lethal ; Genes. 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L</creatorcontrib><title>Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>We have determined the entire sequence of the plasmid K2 from Kluyveromyces lactis which is involved in the maintenance of both killer plasmids in the cell. K2 shares many of the characteristics of the smaller killer plasmid K1: high A+T content (74.7%) and very compact genomic organization. K2 contains ten open reading frames. Some of them overlap on different strands and some on the same strand. Northern blotting of K2 transcripts shows that at least eight ORFs are transcribed. Analysis of the predicted aminoacid sequence of ORF2 from K2 reveals homology with the aminoacid sequence of ORF 1 from K1 and with several viral DNA polymerases. The sequence of K2 from Saccaromyces cerevisiae F102-2 was also determined. Only one nucleotide difference was found between the K2 sequence from the two yeasts. This mutation does not change the genome organization of the plasmid and has only minimal effect on the structure of the encoded proteins.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA, Fungal - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Lethal</subject><subject>Genes. Genome</subject><subject>Kluyveromyces lactis</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomycetales - genetics</subject><subject>Transcription, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9LHDEUx0NR7Gp77a2Qg3ibNW_yY2YOPRTRVRS82HPIJm80bWayTWaF9a83i8uiJwMhge_n-95LvoT8ADYH1vHz0aRzUHPgc9kq_oXMgKu6Ep2qD8iMcSYrYKL9So5z_ssYCJDiiBxxJgrXzcjNAsc4II3p0Yz-xUw-jjT2dHpC-s-HgImugsmDd3S1uIWa9ikO9DasN89YbhuLmQZjJ5-_kcPehIzfd-cJ-XN1-XBxXd3dL24uft9VVgqYKmmYqxshy-oUOsd6NMvGlSGxbpSQCI5Z5zhvrVK168tWbIkcuqWDHiU_Ib_e6q7WywGdxXFKJuhV8oNJGx2N1x-V0T_px_isOVctF8V_tvOn-H-NedKDzxZDMCPGddZNW3rXUn4KQvljLjn7HJQgWAvb1vM30KaYc8J-PzUwvU1TlzQ1KA1cb9Mshp_v37rHd_EV_XSnm2xN6JMZrc97rGkaKUHyV8SDqLs</recordid><startdate>19880711</startdate><enddate>19880711</enddate><creator>TOMMASINO, M</creator><creator>RICCI, S</creator><creator>GALEOTTI, C. L</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19880711</creationdate><title>Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis</title><author>TOMMASINO, M ; RICCI, S ; GALEOTTI, C. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-5a0d274555596edd0feab7d104e27645e1d0cdd338c662df62d60be319bd1fe53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA, Fungal - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Lethal</topic><topic>Genes. Genome</topic><topic>Kluyveromyces lactis</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomycetales - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TOMMASINO, M</creatorcontrib><creatorcontrib>RICCI, S</creatorcontrib><creatorcontrib>GALEOTTI, C. 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L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1988-07-11</date><risdate>1988</risdate><volume>16</volume><issue>13</issue><spage>5863</spage><epage>5878</epage><pages>5863-5878</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>We have determined the entire sequence of the plasmid K2 from Kluyveromyces lactis which is involved in the maintenance of both killer plasmids in the cell. K2 shares many of the characteristics of the smaller killer plasmid K1: high A+T content (74.7%) and very compact genomic organization. K2 contains ten open reading frames. Some of them overlap on different strands and some on the same strand. Northern blotting of K2 transcripts shows that at least eight ORFs are transcribed. Analysis of the predicted aminoacid sequence of ORF2 from K2 reveals homology with the aminoacid sequence of ORF 1 from K1 and with several viral DNA polymerases. The sequence of K2 from Saccaromyces cerevisiae F102-2 was also determined. Only one nucleotide difference was found between the K2 sequence from the two yeasts. This mutation does not change the genome organization of the plasmid and has only minimal effect on the structure of the encoded proteins.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3041369</pmid><doi>10.1093/nar/16.13.5863</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1988-07, Vol.16 (13), p.5863-5878 |
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| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences DNA, Fungal - analysis Fundamental and applied biological sciences. Psychology Genes, Lethal Genes. Genome Kluyveromyces lactis Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids Saccharomyces cerevisiae - genetics Saccharomycetales - genetics Transcription, Genetic |
| title | Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis |
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