Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response

Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response

Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of...

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Veröffentlicht in:Clinical and translational gastroenterology 2012-05, Vol.3 (5), p.e15-e15
Hauptverfasser: Symonds, Erin L, O'Mahony, Caitlin, Lapthorne, Susan, O'Mahony, David, Sharry, John Mac, O'Mahony, Liam, Shanahan, Fergus
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container_title Clinical and translational gastroenterology
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creator Symonds, Erin L
O'Mahony, Caitlin
Lapthorne, Susan
O'Mahony, David
Sharry, John Mac
O'Mahony, Liam
Shanahan, Fergus
description Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage. BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10(2)-10(8) colony-forming unit (CFU)) and durations (10(6) CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10(6) CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase. S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P
doi_str_mv 10.1038/ctg.2012.9
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title Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response
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