Junctions between i-motif tetramers in supramolecular structures
The symmetry of i-motif tetramers gives to cytidine-rich oligonucleotides the capacity to associate into supramolecular structures (sms). In order to determine how the tetramers are linked together in such structures, we have measured by gel filtration chromatography and NMR the formation and dissoc...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 2012-06, Vol.40 (11), p.5162-5170 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5170 |
|---|---|
| container_issue | 11 |
| container_start_page | 5162 |
| container_title | Nucleic acids research |
| container_volume | 40 |
| creator | Guittet, Eric Renciuk, Daniel Leroy, Jean-Louis |
| description | The symmetry of i-motif tetramers gives to cytidine-rich oligonucleotides the capacity to associate into supramolecular structures (sms). In order to determine how the tetramers are linked together in such structures, we have measured by gel filtration chromatography and NMR the formation and dissociation kinetics of sms built by oligonucleotides containing two short C stretches separated by a non-cytidine-base. We show that a stretch of only two cytidines either at the 3'- or 5'-end is long enough to link the tetramers into sms. The analysis of the properties of sms formed by oligonucleotides differing by the length of the oligo-C stretches, the sequence orientation and the nature of the non-C base provides a model of the junction connecting the tetramers in sms. |
| doi_str_mv | 10.1093/nar/gks161 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3367196</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1019100235</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-90e6f2fb506d7627f4f587f0b6d098293bf228d2feebee4f8f7ba65459463a0c3</originalsourceid><addsrcrecordid>eNqNkU1PHDEMhqMKVBbaS38AmiMgDdj5mskFgRAtoJV6ac9RZtaBofOxJBkq_j1BS1HbEyfL9uPXtl7GviAcIxhxMrpwcvsrosYPbIFC81IazbfYAgSoEkHWO2w3xnsAlKjkR7bDeaYqYRbs7GYe29RNYywaSr-JxqIrhyl1vkiUghsoxKIbizivczL11M69C0VMYW7THCh-Ytve9ZE-v8Y99vPr5Y-Lq3L5_dv1xfmybBViKg2Q9tw3CvSqyru99KquPDR6BabmRjSe83rFPVFDJH3tq8ZpJZWRWjhoxR473eiu52agVUtjvq6369ANLjzZyXX2387Y3dnb6dEKoSs0OgscbgTu_hu7Ol_alxpAxTko-YiZPXhdFqaHmWKyQxdb6ns30jRHi8Br4FKgfgeKBgG4UBk92qBtmGIM5N_OQLAvTtrspN04meH9v_99Q_9YJ54BGtObAw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1019100235</pqid></control><display><type>article</type><title>Junctions between i-motif tetramers in supramolecular structures</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Oxford Journals Open Access Collection</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Guittet, Eric ; Renciuk, Daniel ; Leroy, Jean-Louis</creator><creatorcontrib>Guittet, Eric ; Renciuk, Daniel ; Leroy, Jean-Louis</creatorcontrib><description>The symmetry of i-motif tetramers gives to cytidine-rich oligonucleotides the capacity to associate into supramolecular structures (sms). In order to determine how the tetramers are linked together in such structures, we have measured by gel filtration chromatography and NMR the formation and dissociation kinetics of sms built by oligonucleotides containing two short C stretches separated by a non-cytidine-base. We show that a stretch of only two cytidines either at the 3'- or 5'-end is long enough to link the tetramers into sms. The analysis of the properties of sms formed by oligonucleotides differing by the length of the oligo-C stretches, the sequence orientation and the nature of the non-C base provides a model of the junction connecting the tetramers in sms.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/gks161</identifier><identifier>PMID: 22362739</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Chemical Sciences ; Chromatography ; Cytidine - chemistry ; Dimerization ; Filtration ; Kinetics ; Models, Molecular ; N.M.R ; Nucleotide Motifs ; Oligonucleotides ; Oligonucleotides - chemistry ; Organic chemistry ; Structural Biology</subject><ispartof>Nucleic acids research, 2012-06, Vol.40 (11), p.5162-5170</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>The Author(s) 2012. Published by Oxford University Press. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-90e6f2fb506d7627f4f587f0b6d098293bf228d2feebee4f8f7ba65459463a0c3</citedby><cites>FETCH-LOGICAL-c511t-90e6f2fb506d7627f4f587f0b6d098293bf228d2feebee4f8f7ba65459463a0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367196/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367196/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22362739$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00722054$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Guittet, Eric</creatorcontrib><creatorcontrib>Renciuk, Daniel</creatorcontrib><creatorcontrib>Leroy, Jean-Louis</creatorcontrib><title>Junctions between i-motif tetramers in supramolecular structures</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The symmetry of i-motif tetramers gives to cytidine-rich oligonucleotides the capacity to associate into supramolecular structures (sms). In order to determine how the tetramers are linked together in such structures, we have measured by gel filtration chromatography and NMR the formation and dissociation kinetics of sms built by oligonucleotides containing two short C stretches separated by a non-cytidine-base. We show that a stretch of only two cytidines either at the 3'- or 5'-end is long enough to link the tetramers into sms. The analysis of the properties of sms formed by oligonucleotides differing by the length of the oligo-C stretches, the sequence orientation and the nature of the non-C base provides a model of the junction connecting the tetramers in sms.</description><subject>Chemical Sciences</subject><subject>Chromatography</subject><subject>Cytidine - chemistry</subject><subject>Dimerization</subject><subject>Filtration</subject><subject>Kinetics</subject><subject>Models, Molecular</subject><subject>N.M.R</subject><subject>Nucleotide Motifs</subject><subject>Oligonucleotides</subject><subject>Oligonucleotides - chemistry</subject><subject>Organic chemistry</subject><subject>Structural Biology</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1PHDEMhqMKVBbaS38AmiMgDdj5mskFgRAtoJV6ac9RZtaBofOxJBkq_j1BS1HbEyfL9uPXtl7GviAcIxhxMrpwcvsrosYPbIFC81IazbfYAgSoEkHWO2w3xnsAlKjkR7bDeaYqYRbs7GYe29RNYywaSr-JxqIrhyl1vkiUghsoxKIbizivczL11M69C0VMYW7THCh-Ytve9ZE-v8Y99vPr5Y-Lq3L5_dv1xfmybBViKg2Q9tw3CvSqyru99KquPDR6BabmRjSe83rFPVFDJH3tq8ZpJZWRWjhoxR473eiu52agVUtjvq6369ANLjzZyXX2387Y3dnb6dEKoSs0OgscbgTu_hu7Ol_alxpAxTko-YiZPXhdFqaHmWKyQxdb6ns30jRHi8Br4FKgfgeKBgG4UBk92qBtmGIM5N_OQLAvTtrspN04meH9v_99Q_9YJ54BGtObAw</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Guittet, Eric</creator><creator>Renciuk, Daniel</creator><creator>Leroy, Jean-Louis</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>20120601</creationdate><title>Junctions between i-motif tetramers in supramolecular structures</title><author>Guittet, Eric ; Renciuk, Daniel ; Leroy, Jean-Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-90e6f2fb506d7627f4f587f0b6d098293bf228d2feebee4f8f7ba65459463a0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Chemical Sciences</topic><topic>Chromatography</topic><topic>Cytidine - chemistry</topic><topic>Dimerization</topic><topic>Filtration</topic><topic>Kinetics</topic><topic>Models, Molecular</topic><topic>N.M.R</topic><topic>Nucleotide Motifs</topic><topic>Oligonucleotides</topic><topic>Oligonucleotides - chemistry</topic><topic>Organic chemistry</topic><topic>Structural Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guittet, Eric</creatorcontrib><creatorcontrib>Renciuk, Daniel</creatorcontrib><creatorcontrib>Leroy, Jean-Louis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guittet, Eric</au><au>Renciuk, Daniel</au><au>Leroy, Jean-Louis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Junctions between i-motif tetramers in supramolecular structures</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>40</volume><issue>11</issue><spage>5162</spage><epage>5170</epage><pages>5162-5170</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>The symmetry of i-motif tetramers gives to cytidine-rich oligonucleotides the capacity to associate into supramolecular structures (sms). In order to determine how the tetramers are linked together in such structures, we have measured by gel filtration chromatography and NMR the formation and dissociation kinetics of sms built by oligonucleotides containing two short C stretches separated by a non-cytidine-base. We show that a stretch of only two cytidines either at the 3'- or 5'-end is long enough to link the tetramers into sms. The analysis of the properties of sms formed by oligonucleotides differing by the length of the oligo-C stretches, the sequence orientation and the nature of the non-C base provides a model of the junction connecting the tetramers in sms.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>22362739</pmid><doi>10.1093/nar/gks161</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 2012-06, Vol.40 (11), p.5162-5170 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3367196 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Oxford Journals Open Access Collection; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Chemical Sciences Chromatography Cytidine - chemistry Dimerization Filtration Kinetics Models, Molecular N.M.R Nucleotide Motifs Oligonucleotides Oligonucleotides - chemistry Organic chemistry Structural Biology |
| title | Junctions between i-motif tetramers in supramolecular structures |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T04%3A00%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Junctions%20between%20i-motif%20tetramers%20in%20supramolecular%20structures&rft.jtitle=Nucleic%20acids%20research&rft.au=Guittet,%20Eric&rft.date=2012-06-01&rft.volume=40&rft.issue=11&rft.spage=5162&rft.epage=5170&rft.pages=5162-5170&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/gks161&rft_dat=%3Cproquest_pubme%3E1019100235%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1019100235&rft_id=info:pmid/22362739&rfr_iscdi=true |