Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans
A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino aci...
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| Veröffentlicht in: | Nucleic acids research 1988-05, Vol.16 (10), p.4447-4463 |
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| container_title | Nucleic acids research |
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| creator | YASUI, A TAKAO, M OIKAWA, A KIENER, A WALSH, C. T EKER, A. P. M |
| description | A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure. |
| doi_str_mv | 10.1093/nar/16.10.4447 |
| format | Article |
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A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/16.10.4447</identifier><identifier>PMID: 2837735</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; Anacystis nidulans ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; Cyanobacteria ; Cyanobacteria - enzymology ; Cyanobacteria - genetics ; Cyanophyta ; Deoxyribodipyrimidine Photo-Lyase - genetics ; DNA Restriction Enzymes ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic engineering ; Genetic technics ; Lyases - genetics ; Methods. Procedures. 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T</creatorcontrib><creatorcontrib>EKER, A. P. M</creatorcontrib><title>Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure.</description><subject>Amino Acid Sequence</subject><subject>Anacystis nidulans</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - enzymology</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanophyta</subject><subject>Deoxyribodipyrimidine Photo-Lyase - genetics</subject><subject>DNA Restriction Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Lyases - genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuLFDEQxoMo67h69SbkIN56Nu90Dh6WwRcseNGbEGrymIl0J2PSLYx_vT3MMOhpT1XF9_uKKj6EXlOypsTwuwz1jqqlXwsh9BO0olyxThjFnqIV4UR2lIj-OXrR2k9CqKBS3KAb1nOtuVyhH5uh5JR3GLLHbg8V3BRq-gNTKhmXiAEf9mUqwxFawLuQA461jHjaB-yOkMv2bJhHfJ_BHduUGs7JzwPk9hI9izC08OpSb9H3jx--bT53D18_fdncP3ROcDl10htPWODGRCkkU0JH57RivI9MK7OonHvXUwDpt55IGaR3lEcTufCeKH6L3p_3HubtGLwLeaow2ENNI9SjLZDs_0pOe7srvy3nSgm6-N9d_LX8mkOb7JiaC8PyQyhzs7pnihnVPwoyQrWRRj8KUsk0MfoErs-gq6W1GuL1akrsKWC7BGypOo2ngBfDm39_veKXRBf97UWH5mCIFbJL7YpppaToNf8LkDewiA</recordid><startdate>19880525</startdate><enddate>19880525</enddate><creator>YASUI, A</creator><creator>TAKAO, M</creator><creator>OIKAWA, A</creator><creator>KIENER, A</creator><creator>WALSH, C. 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T</au><au>EKER, A. P. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1988-05-25</date><risdate>1988</risdate><volume>16</volume><issue>10</issue><spage>4447</spage><epage>4463</epage><pages>4447-4463</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. 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| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1988-05, Vol.16 (10), p.4447-4463 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_336641 |
| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Amino Acid Sequence Anacystis nidulans Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular Cyanobacteria Cyanobacteria - enzymology Cyanobacteria - genetics Cyanophyta Deoxyribodipyrimidine Photo-Lyase - genetics DNA Restriction Enzymes Fundamental and applied biological sciences. Psychology Genes Genetic engineering Genetic technics Lyases - genetics Methods. Procedures. Technologies Molecular cloning Molecular Sequence Data Nucleic Acid Hybridization Sequence Homology, Nucleic Acid |
| title | Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans |
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