Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materi...
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| description | Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers of cumulus cells and cytoplasm or cytoplasm uniformity and colors. This requires considerable experience to better identify oocyte quality because of the intense subjectivity involved (Gordon (2003), Madison et al. (1992) and De Loos et al. (1992)). BCB staining is a function of glucose-6-phosphate dehydrogenase (G6PD) activity, an enzyme synthesized in developing oocytes, which decreases in activity with maturation. Therefore, unstained oocytes (BCB−) are high in G6PD activity, while the less mature oocytes stains are deep blue (BCB+) due to insuffcient G6PD activity to decolorize the BCB dye. |
| doi_str_mv | 10.1155/2012/161372 |
| format | Article |
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Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers of cumulus cells and cytoplasm or cytoplasm uniformity and colors. This requires considerable experience to better identify oocyte quality because of the intense subjectivity involved (Gordon (2003), Madison et al. (1992) and De Loos et al. (1992)). BCB staining is a function of glucose-6-phosphate dehydrogenase (G6PD) activity, an enzyme synthesized in developing oocytes, which decreases in activity with maturation. Therefore, unstained oocytes (BCB−) are high in G6PD activity, while the less mature oocytes stains are deep blue (BCB+) due to insuffcient G6PD activity to decolorize the BCB dye.</description><identifier>ISSN: 1110-7243</identifier><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 1110-7251</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2012/161372</identifier><identifier>PMID: 22675245</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Analysis of Variance ; Animals ; Biotechnology ; Cell culture ; Cell Culture Techniques ; Cell Size - drug effects ; Cells, Cultured ; Coloring Agents - chemistry ; Cytoplasm ; Egg Proteins - genetics ; Egg Proteins - metabolism ; Embryos ; Female ; Glucosephosphate Dehydrogenase - genetics ; Glucosephosphate Dehydrogenase - metabolism ; Glutathione - metabolism ; Mineral oils ; Oocytes - chemistry ; Oocytes - cytology ; Oocytes - metabolism ; Ovary - cytology ; Ovary - metabolism ; Oxazines - chemistry ; Polymerase Chain Reaction ; Polyvinyl alcohol ; Proteins ; Sheep ; Staining and Labeling - methods</subject><ispartof>BioMed research international, 2012-01, Vol.2012 (2012), p.1-7</ispartof><rights>Copyright © 2012 Liqin Wang et al.</rights><rights>Copyright © 2012 Liqin Wang et al. Liqin Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2012 Liqin Wang et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-609cea98da72177889882ad00548682fc8a80fa0b855aa33dd75ce4b683c2b113</citedby><cites>FETCH-LOGICAL-c565t-609cea98da72177889882ad00548682fc8a80fa0b855aa33dd75ce4b683c2b113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366259/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366259/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22675245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Upham, Brad</contributor><creatorcontrib>Huang, Juncheng</creatorcontrib><creatorcontrib>Wang, Liqin</creatorcontrib><creatorcontrib>Chen, Tong</creatorcontrib><creatorcontrib>Zhao, Yuncheng</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Lin, Jiapeng</creatorcontrib><title>Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining</title><title>BioMed research international</title><addtitle>J Biomed Biotechnol</addtitle><description>Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers of cumulus cells and cytoplasm or cytoplasm uniformity and colors. This requires considerable experience to better identify oocyte quality because of the intense subjectivity involved (Gordon (2003), Madison et al. (1992) and De Loos et al. (1992)). BCB staining is a function of glucose-6-phosphate dehydrogenase (G6PD) activity, an enzyme synthesized in developing oocytes, which decreases in activity with maturation. Therefore, unstained oocytes (BCB−) are high in G6PD activity, while the less mature oocytes stains are deep blue (BCB+) due to insuffcient G6PD activity to decolorize the BCB dye.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell Size - drug effects</subject><subject>Cells, Cultured</subject><subject>Coloring Agents - chemistry</subject><subject>Cytoplasm</subject><subject>Egg Proteins - genetics</subject><subject>Egg Proteins - metabolism</subject><subject>Embryos</subject><subject>Female</subject><subject>Glucosephosphate Dehydrogenase - genetics</subject><subject>Glucosephosphate Dehydrogenase - metabolism</subject><subject>Glutathione - metabolism</subject><subject>Mineral oils</subject><subject>Oocytes - chemistry</subject><subject>Oocytes - cytology</subject><subject>Oocytes - metabolism</subject><subject>Ovary - cytology</subject><subject>Ovary - 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| subjects | Analysis of Variance Animals Biotechnology Cell culture Cell Culture Techniques Cell Size - drug effects Cells, Cultured Coloring Agents - chemistry Cytoplasm Egg Proteins - genetics Egg Proteins - metabolism Embryos Female Glucosephosphate Dehydrogenase - genetics Glucosephosphate Dehydrogenase - metabolism Glutathione - metabolism Mineral oils Oocytes - chemistry Oocytes - cytology Oocytes - metabolism Ovary - cytology Ovary - metabolism Oxazines - chemistry Polymerase Chain Reaction Polyvinyl alcohol Proteins Sheep Staining and Labeling - methods |
| title | Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining |
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