Poly( β-amino ester)–DNA complexes: Time-resolved fluorescence and cellular transfection studies
A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly( β-am...
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| Veröffentlicht in: | Journal of controlled release 2011-09, Vol.154 (2), p.171-176 |
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| creator | Vuorimaa, Elina Ketola, Tiia-Maaria Green, Jordan J. Hanzlíková, Martina Lemmetyinen, Helge Langer, Robert Anderson, Daniel G. Urtti, Arto Yliperttula, Marjo |
| description | A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(
β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(
β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(
β-amino esters) than for 25
kDa polyethylenimines, yet poly(
β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.
[Display omitted] |
| doi_str_mv | 10.1016/j.jconrel.2011.06.016 |
| format | Article |
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β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(
β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(
β-amino esters) than for 25
kDa polyethylenimines, yet poly(
β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.
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β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(
β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(
β-amino esters) than for 25
kDa polyethylenimines, yet poly(
β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.
[Display omitted]</description><subject>binding properties</subject><subject>biodegradability</subject><subject>Biological and medical sciences</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>DNA binding</subject><subject>DNA complexation</subject><subject>esters</subject><subject>fluorescence</subject><subject>Gene delivery</subject><subject>General pharmacology</subject><subject>genes</subject><subject>Medical sciences</subject><subject>Nanoparticles</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Poly( β-amino esters)</subject><subject>polymers</subject><subject>Polymers - metabolism</subject><subject>Protein Binding - physiology</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Time Factors</subject><subject>Time-resolved fluorescence spectroscopy</subject><subject>transfection</subject><subject>Transfection - methods</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9u1TAQxi0Eoo_CEYBsEGWRYCdOYrMAVeWvVAES7dpynHHxkxM_7OSJ7rgDN-EgHIKTMNF7FNjAypqZ34y_mY-Qu4wWjLLm8bpYmzBG8EVJGStoU2D2Glkx0VY5l7K-TlaYEXnV1PKA3EppTSmtK97eJAcla6SUpVgR8z74y6Ps-7dcD24MGaQJ4qMfX74-f3ucmTBsPHyG9CQ7cwPkEVLwW-gz6-eAgYHRQKbHPjPg_ex1zKaox2TBTC6MWZrm3kG6TW5Y7RPc2b-H5Pzli7OT1_npu1dvTo5Pc1OLeso7w7ueNZ0WxjDWdr3obMc4K21XLTVdNRagNp1grTalBskFhtYaW_IOdHVInu7mbuZugB7VoRqvNtENOl6qoJ36uzK6j-oibFVVNWXZSBzwcD8ghk8znkINLi2r6RHCnJSkLROMc4Hk0T9JvC_nkkvKEa13qIkhpQj2ShCjarFSrdXeSrVYqWijMIt99_7c5qrrl3cIPNgDOhntLV7euPSbQwFVIxYB93ec1UHpi4jM-Qf8qaYUtxF8IZ7tCEB3tg6iSsYt3vYuopWqD-4_Yn8Ch93ORA</recordid><startdate>20110905</startdate><enddate>20110905</enddate><creator>Vuorimaa, Elina</creator><creator>Ketola, Tiia-Maaria</creator><creator>Green, Jordan J.</creator><creator>Hanzlíková, Martina</creator><creator>Lemmetyinen, Helge</creator><creator>Langer, Robert</creator><creator>Anderson, Daniel G.</creator><creator>Urtti, Arto</creator><creator>Yliperttula, Marjo</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20110905</creationdate><title>Poly( β-amino ester)–DNA complexes: Time-resolved fluorescence and cellular transfection studies</title><author>Vuorimaa, Elina ; Ketola, Tiia-Maaria ; Green, Jordan J. ; Hanzlíková, Martina ; Lemmetyinen, Helge ; Langer, Robert ; Anderson, Daniel G. ; Urtti, Arto ; Yliperttula, Marjo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c585t-bc4bd16ba8cc117bd8bfb1412fb3bc4ba36fee5cb817ac2ae948e5cffcf24bea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>binding properties</topic><topic>biodegradability</topic><topic>Biological and medical sciences</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA - metabolism</topic><topic>DNA binding</topic><topic>DNA complexation</topic><topic>esters</topic><topic>fluorescence</topic><topic>Gene delivery</topic><topic>General pharmacology</topic><topic>genes</topic><topic>Medical sciences</topic><topic>Nanoparticles</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Poly( β-amino esters)</topic><topic>polymers</topic><topic>Polymers - metabolism</topic><topic>Protein Binding - physiology</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Time Factors</topic><topic>Time-resolved fluorescence spectroscopy</topic><topic>transfection</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vuorimaa, Elina</creatorcontrib><creatorcontrib>Ketola, Tiia-Maaria</creatorcontrib><creatorcontrib>Green, Jordan J.</creatorcontrib><creatorcontrib>Hanzlíková, Martina</creatorcontrib><creatorcontrib>Lemmetyinen, Helge</creatorcontrib><creatorcontrib>Langer, Robert</creatorcontrib><creatorcontrib>Anderson, Daniel G.</creatorcontrib><creatorcontrib>Urtti, Arto</creatorcontrib><creatorcontrib>Yliperttula, Marjo</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vuorimaa, Elina</au><au>Ketola, Tiia-Maaria</au><au>Green, Jordan J.</au><au>Hanzlíková, Martina</au><au>Lemmetyinen, Helge</au><au>Langer, Robert</au><au>Anderson, Daniel G.</au><au>Urtti, Arto</au><au>Yliperttula, Marjo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Poly( β-amino ester)–DNA complexes: Time-resolved fluorescence and cellular transfection studies</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>2011-09-05</date><risdate>2011</risdate><volume>154</volume><issue>2</issue><spage>171</spage><epage>176</epage><pages>171-176</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><coden>JCREEC</coden><abstract>A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(
β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(
β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(
β-amino esters) than for 25
kDa polyethylenimines, yet poly(
β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.
[Display omitted]</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>21699928</pmid><doi>10.1016/j.jconrel.2011.06.016</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of controlled release, 2011-09, Vol.154 (2), p.171-176 |
| issn | 0168-3659 1873-4995 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3362269 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | binding properties biodegradability Biological and medical sciences DNA DNA - genetics DNA - metabolism DNA binding DNA complexation esters fluorescence Gene delivery General pharmacology genes Medical sciences Nanoparticles Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Poly( β-amino esters) polymers Polymers - metabolism Protein Binding - physiology Spectrometry, Fluorescence - methods Time Factors Time-resolved fluorescence spectroscopy transfection Transfection - methods |
| title | Poly( β-amino ester)–DNA complexes: Time-resolved fluorescence and cellular transfection studies |
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