Point mutation in codon 61 of N-RAS genes in human myeloma cell lines
Two myeloma cell lines were established from a patient. One that originated from the bone marrow (ILXOB) requires interleukin 6 (IL-6) for its growth and the other that originated from the pleural effusion (KO44) proliferates in the absence of IL-6 in the culture medium. To examine ras oncogene acti...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 1989-11, Vol.17 (21), p.8867-8867 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8867 |
|---|---|
| container_issue | 21 |
| container_start_page | 8867 |
| container_title | Nucleic acids research |
| container_volume | 17 |
| creator | SAWADA, M SHIMIZU, S ARAI, T KONDA, S ENOMOTO, N DATE, T |
| description | Two myeloma cell lines were established from a patient. One that originated from the bone marrow (ILXOB) requires interleukin 6 (IL-6) for its growth and the other that originated from the pleural effusion (KO44) proliferates in the absence of IL-6 in the culture medium. To examine ras oncogene activation in both cell lines, the partial nucleotide sequence of N-ras genes was determined by a direct sequencing method using polymerase chain reaction products. Analysis in exon 1 of N-ras genes from both cell lines revealed that neither had any mutations around codon 12 or 13 region. In exon 2, however, two double ladders, G and T, were found at the first nucleotide of codon 61 in both cell lines. |
| doi_str_mv | 10.1093/nar/17.21.8867 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_335060</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79345817</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-a6a265227a53664e74bfa915b89369e32521f71c022817cb59e7a54e6267f073</originalsourceid><addsrcrecordid>eNqNkctLAzEQxoMoWh9Xb0IO4m1rJs_dgwcp9QGiot5DmmZrZDfRza7gf29KS9GTnjLw_b7JzHwIHQMZA6nYeTDdOagxhXFZSrWFRsAkLXgl6TYaEUZEAYSXe2g_pTdCgIPgu2iXylIoASM0fYw-9LgdetP7GLAP2MZ5LiTgWOP74unyGS9ccGkpvQ6tCbj9ck1sDbauaXDjs3aIdmrTJHe0fg_Qy9X0ZXJT3D1c304u7wrLlegLIw2VglJlBJOSO8VntalAzMqKycoxKijUCiyhtARlZ6JyGeVOUqlqotgBuli1fR9mrZtbF_rONPq9863pvnQ0Xv9Wgn_Vi_ipGRNEkuw_W_u7-DG41OvWp-UWJrg4JK0qxkX--U8Q8nkpqH-AgpVlPnUGxyvQdjGlztWbqYHoZZA6B6lBaQp6GWQ2nPzcdYOvk8v66Vo3yZqm7kywPm0wKWWGBPsGtxSkHg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15388857</pqid></control><display><type>article</type><title>Point mutation in codon 61 of N-RAS genes in human myeloma cell lines</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><source>PubMed Central</source><creator>SAWADA, M ; SHIMIZU, S ; ARAI, T ; KONDA, S ; ENOMOTO, N ; DATE, T</creator><creatorcontrib>SAWADA, M ; SHIMIZU, S ; ARAI, T ; KONDA, S ; ENOMOTO, N ; DATE, T</creatorcontrib><description>Two myeloma cell lines were established from a patient. One that originated from the bone marrow (ILXOB) requires interleukin 6 (IL-6) for its growth and the other that originated from the pleural effusion (KO44) proliferates in the absence of IL-6 in the culture medium. To examine ras oncogene activation in both cell lines, the partial nucleotide sequence of N-ras genes was determined by a direct sequencing method using polymerase chain reaction products. Analysis in exon 1 of N-ras genes from both cell lines revealed that neither had any mutations around codon 12 or 13 region. In exon 2, however, two double ladders, G and T, were found at the first nucleotide of codon 61 in both cell lines.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/17.21.8867</identifier><identifier>PMID: 2685751</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; Autoradiography ; Base Sequence ; Biological and medical sciences ; Codon ; Fundamental and applied biological sciences. Psychology ; Genes, ras ; Humans ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; multiple myeloma ; Multiple Myeloma - genetics ; Mutagenesis. Repair ; Mutation ; point mutation ; RNA, Messenger ; Tumor Cells, Cultured</subject><ispartof>Nucleic acids research, 1989-11, Vol.17 (21), p.8867-8867</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-a6a265227a53664e74bfa915b89369e32521f71c022817cb59e7a54e6267f073</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC335060/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC335060/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27911,27912,53778,53780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6665135$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2685751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SAWADA, M</creatorcontrib><creatorcontrib>SHIMIZU, S</creatorcontrib><creatorcontrib>ARAI, T</creatorcontrib><creatorcontrib>KONDA, S</creatorcontrib><creatorcontrib>ENOMOTO, N</creatorcontrib><creatorcontrib>DATE, T</creatorcontrib><title>Point mutation in codon 61 of N-RAS genes in human myeloma cell lines</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Two myeloma cell lines were established from a patient. One that originated from the bone marrow (ILXOB) requires interleukin 6 (IL-6) for its growth and the other that originated from the pleural effusion (KO44) proliferates in the absence of IL-6 in the culture medium. To examine ras oncogene activation in both cell lines, the partial nucleotide sequence of N-ras genes was determined by a direct sequencing method using polymerase chain reaction products. Analysis in exon 1 of N-ras genes from both cell lines revealed that neither had any mutations around codon 12 or 13 region. In exon 2, however, two double ladders, G and T, were found at the first nucleotide of codon 61 in both cell lines.</description><subject>Amino Acid Sequence</subject><subject>Autoradiography</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Codon</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, ras</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>multiple myeloma</subject><subject>Multiple Myeloma - genetics</subject><subject>Mutagenesis. Repair</subject><subject>Mutation</subject><subject>point mutation</subject><subject>RNA, Messenger</subject><subject>Tumor Cells, Cultured</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctLAzEQxoMoWh9Xb0IO4m1rJs_dgwcp9QGiot5DmmZrZDfRza7gf29KS9GTnjLw_b7JzHwIHQMZA6nYeTDdOagxhXFZSrWFRsAkLXgl6TYaEUZEAYSXe2g_pTdCgIPgu2iXylIoASM0fYw-9LgdetP7GLAP2MZ5LiTgWOP74unyGS9ccGkpvQ6tCbj9ck1sDbauaXDjs3aIdmrTJHe0fg_Qy9X0ZXJT3D1c304u7wrLlegLIw2VglJlBJOSO8VntalAzMqKycoxKijUCiyhtARlZ6JyGeVOUqlqotgBuli1fR9mrZtbF_rONPq9863pvnQ0Xv9Wgn_Vi_ipGRNEkuw_W_u7-DG41OvWp-UWJrg4JK0qxkX--U8Q8nkpqH-AgpVlPnUGxyvQdjGlztWbqYHoZZA6B6lBaQp6GWQ2nPzcdYOvk8v66Vo3yZqm7kywPm0wKWWGBPsGtxSkHg</recordid><startdate>19891111</startdate><enddate>19891111</enddate><creator>SAWADA, M</creator><creator>SHIMIZU, S</creator><creator>ARAI, T</creator><creator>KONDA, S</creator><creator>ENOMOTO, N</creator><creator>DATE, T</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7TM</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19891111</creationdate><title>Point mutation in codon 61 of N-RAS genes in human myeloma cell lines</title><author>SAWADA, M ; SHIMIZU, S ; ARAI, T ; KONDA, S ; ENOMOTO, N ; DATE, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-a6a265227a53664e74bfa915b89369e32521f71c022817cb59e7a54e6267f073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Autoradiography</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Codon</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, ras</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>multiple myeloma</topic><topic>Multiple Myeloma - genetics</topic><topic>Mutagenesis. Repair</topic><topic>Mutation</topic><topic>point mutation</topic><topic>RNA, Messenger</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SAWADA, M</creatorcontrib><creatorcontrib>SHIMIZU, S</creatorcontrib><creatorcontrib>ARAI, T</creatorcontrib><creatorcontrib>KONDA, S</creatorcontrib><creatorcontrib>ENOMOTO, N</creatorcontrib><creatorcontrib>DATE, T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SAWADA, M</au><au>SHIMIZU, S</au><au>ARAI, T</au><au>KONDA, S</au><au>ENOMOTO, N</au><au>DATE, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Point mutation in codon 61 of N-RAS genes in human myeloma cell lines</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1989-11-11</date><risdate>1989</risdate><volume>17</volume><issue>21</issue><spage>8867</spage><epage>8867</epage><pages>8867-8867</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Two myeloma cell lines were established from a patient. One that originated from the bone marrow (ILXOB) requires interleukin 6 (IL-6) for its growth and the other that originated from the pleural effusion (KO44) proliferates in the absence of IL-6 in the culture medium. To examine ras oncogene activation in both cell lines, the partial nucleotide sequence of N-ras genes was determined by a direct sequencing method using polymerase chain reaction products. Analysis in exon 1 of N-ras genes from both cell lines revealed that neither had any mutations around codon 12 or 13 region. In exon 2, however, two double ladders, G and T, were found at the first nucleotide of codon 61 in both cell lines.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2685751</pmid><doi>10.1093/nar/17.21.8867</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1989-11, Vol.17 (21), p.8867-8867 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_335060 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Amino Acid Sequence Autoradiography Base Sequence Biological and medical sciences Codon Fundamental and applied biological sciences. Psychology Genes, ras Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data multiple myeloma Multiple Myeloma - genetics Mutagenesis. Repair Mutation point mutation RNA, Messenger Tumor Cells, Cultured |
| title | Point mutation in codon 61 of N-RAS genes in human myeloma cell lines |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T11%3A34%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Point%20mutation%20in%20codon%2061%20of%20N-RAS%20genes%20in%20human%20myeloma%20cell%20lines&rft.jtitle=Nucleic%20acids%20research&rft.au=SAWADA,%20M&rft.date=1989-11-11&rft.volume=17&rft.issue=21&rft.spage=8867&rft.epage=8867&rft.pages=8867-8867&rft.issn=0305-1048&rft.eissn=1362-4962&rft.coden=NARHAD&rft_id=info:doi/10.1093/nar/17.21.8867&rft_dat=%3Cproquest_pubme%3E79345817%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15388857&rft_id=info:pmid/2685751&rfr_iscdi=true |