Facing Current Quantification Challenges in Protein Microarrays
The proteome is highly variable and differs from cell to cell. The reasons are posttranslational modifications, splice variants, and polymorphisms. Techniques like next-generation sequencing can only give an inadequate picture of the protein status of a cell. Protein microarrays are able to track th...
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| Veröffentlicht in: | BioMed research international 2012-01, Vol.2012 (2012), p.1-8 |
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| creator | Wellhausen, Robert Seitz, Harald |
| description | The proteome is highly variable and differs from cell to cell. The reasons are posttranslational modifications, splice variants, and polymorphisms. Techniques like next-generation sequencing can only give an inadequate picture of the protein status of a cell. Protein microarrays are able to track these changes on the level they occur: the proteomic level. Therefore, protein microarrays are powerful tools for relative protein quantification, to unveil new interaction partners and to track posttranslational modifications. This papers gives an overview on current protein microarray techniques and discusses recent advances in relative protein quantification. |
| doi_str_mv | 10.1155/2012/831347 |
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The reasons are posttranslational modifications, splice variants, and polymorphisms. Techniques like next-generation sequencing can only give an inadequate picture of the protein status of a cell. Protein microarrays are able to track these changes on the level they occur: the proteomic level. Therefore, protein microarrays are powerful tools for relative protein quantification, to unveil new interaction partners and to track posttranslational modifications. This papers gives an overview on current protein microarray techniques and discusses recent advances in relative protein quantification.</description><identifier>ISSN: 1110-7243</identifier><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 1110-7251</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2012/831347</identifier><identifier>PMID: 22619499</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Alternative splicing ; Biomedical research ; Humans ; Protein Array Analysis - methods ; Proteins - analysis ; Proteomics - methods ; Review ; Sensitivity and Specificity</subject><ispartof>BioMed research international, 2012-01, Vol.2012 (2012), p.1-8</ispartof><rights>Copyright © 2012 Robert Wellhausen and Harald Seitz.</rights><rights>Copyright © 2012 Robert Wellhausen and Harald Seitz. Robert Wellhausen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2012 R. Wellhausen and H. Seitz. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-6ed512497f559aaadec93f393d42ce3f37bd8e7e539b222f8de39c3e79f959a83</citedby><cites>FETCH-LOGICAL-c499t-6ed512497f559aaadec93f393d42ce3f37bd8e7e539b222f8de39c3e79f959a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348655/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348655/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53768,53770</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22619499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sun, Zhirong</contributor><creatorcontrib>Wellhausen, Robert</creatorcontrib><creatorcontrib>Seitz, Harald</creatorcontrib><title>Facing Current Quantification Challenges in Protein Microarrays</title><title>BioMed research international</title><addtitle>J Biomed Biotechnol</addtitle><description>The proteome is highly variable and differs from cell to cell. 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| subjects | Alternative splicing Biomedical research Humans Protein Array Analysis - methods Proteins - analysis Proteomics - methods Review Sensitivity and Specificity |
| title | Facing Current Quantification Challenges in Protein Microarrays |
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