Joining of nonhomologous DNA double strand breaks in vitro

Extracts of Xenopus laevis eggs can efficiently join ends of duplex DNA that differ in structure and sequence. This was analysed by recircularisation of linear plasmid DNA molecules with dissimilar termini, generated by successive cuts with two different restriction enzymes within the pSP65 polylink...

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Veröffentlicht in:	Nucleic acids research 1988-02, Vol.16 (3), p.907-924
Hauptverfasser:	PFEIFFER, P, VIELMETTER, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Base Composition Biological and medical sciences Cell-Free System DNA - metabolism DNA Damage DNA Repair DNA, Circular - metabolism Dna, deoxyribonucleoproteins DNA, Recombinant Fundamental and applied biological sciences. Psychology Models, Genetic Nucleic acids Oocytes - metabolism Plasmids Xenopus laevis
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