Validation of a Microscale Extraction and High Throughput UHPLC-QTOF-MS Analysis Method for Huperzine A in Huperzia: Microscale UHPLC-MS Analysis of Huperzine A

Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase-inhibiting alkaloid, from as little as 3 mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95 % recovery. We also validated a novel UHPLC-QTOF-MS...

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Veröffentlicht in:Biomedical chromatography 2012-01, Vol.26 (10), p.1191-1195
Hauptverfasser: Cuthbertson, Daniel, Piljac-Žegarac, Jasenka, Lange, Bernd Markus
Format: Artikel
Sprache:eng
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